Difference between revisions of "Team:NYMU-Taipei/Model"
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| − | We used 3 models in our project, namely FRET model, mCherry expression model and promoter selection model. | + | <h3>We used 3 models in our project, namely FRET model, mCherry expression model and promoter selection model.</h3> |
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Revision as of 03:27, 26 September 2018
HAIR TO STAY
We used 3 models in our project, namely FRET model, mCherry expression model and promoter selection model.
FRET Model
Objective
This model aims to find out how much FRET protein should be added into our screening system.
Method
Chemical equilibrium is used to determine the florescence level and the minimal FRET protein activity required to produce the florescence that can be detected. We assume the portion of active protein in all protein is constant.
Result
The optimal ratio of the amount of one FRET protein to that of the other is the following:
The figures in the table indicates the optimal ratio of the protein on the top over the protein on the left.
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