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Revision as of 03:31, 26 September 2018




FRET Model

Objective

This model aims to find out how much FRET protein should be added into our screening system.

Method

Chemical equilibrium is used to determine the florescence level and the minimal FRET protein activity required to produce the florescence that can be detected. We assume the portion of active protein in all protein is constant.

Result

The optimal ratio of the amount of one FRET protein to that of the other is the following:

The figures in the table indicates the optimal ratio of the protein on the top over the protein on the left.

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