Difference between revisions of "Team:NYMU-Taipei/Model"
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<h3>Result</h3> | <h3>Result</h3> | ||
<p >The optimal ratio of the amount of one FRET protein to that of the other is the following:</p> | <p >The optimal ratio of the amount of one FRET protein to that of the other is the following:</p> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/e/e2/T--NYMU-Taipei--model-2.png"> |
<p >The figures in the table indicates the optimal ratio of the protein on the top over the protein on the left.</p> | <p >The figures in the table indicates the optimal ratio of the protein on the top over the protein on the left.</p> | ||
<p class="detailtrigger" id="1t" onclick="detail(event)">Click Here For More Info</p> | <p class="detailtrigger" id="1t" onclick="detail(event)">Click Here For More Info</p> | ||
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<div class="story section"> | <div class="story section"> | ||
<h2 id="2" class="story-title subtitle">mCherry Expression Model</h2> | <h2 id="2" class="story-title subtitle">mCherry Expression Model</h2> | ||
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<p>[3]</p> | <p>[3]</p> | ||
<p></p> | <p></p> | ||
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<div class="paragraphs"> | <div class="paragraphs"> | ||
<h3>Objective</h3> | <h3>Objective</h3> | ||
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<h2 id="3" class="story-title subtitle">Promoter Selection Model</h2> | <h2 id="3" class="story-title subtitle">Promoter Selection Model</h2> | ||
<div class="paragraphs"> | <div class="paragraphs"> | ||
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<p>[2]</p> | <p>[2]</p> | ||
<h3>Objective</h3> | <h3>Objective</h3> | ||
Revision as of 04:08, 26 September 2018
FRET Model
Objective
This model aims to find out how much FRET protein should be added into our screening system.
Method
Chemical equilibrium is used to determine the florescence level and the minimal FRET protein activity required to produce the florescence that can be detected. We assume the portion of active protein in all protein is constant.
Result
The optimal ratio of the amount of one FRET protein to that of the other is the following:
The figures in the table indicates the optimal ratio of the protein on the top over the protein on the left.
Click Here For More Info
mCherry Expression Model
[3]
Objective
We made a gene that connects DKK1 promoter to mCherry. The gene expression rate of DKK1 promoter is affected by testosterone activity and affects the expression level of mCherry. The expression level of mCherry should be greater than a threshold so that its florescence can be detected by devices. In order to achieve this threshold, sufficient amount of testosterone should be in our screening system; this is the sensitivity of our screening system. This model aims to find out the sensitivity of our screening system.
Method
We simulate the kinetics of transcription signal and then simulate the expression of DKK1. Testosterone efficiency is used in this model instead of testosterone activity. Florescence decay is not considered in this model because ……..
Result
[DKK1] = 5 + 0.006 [DHT]^2
where [DKK1] indicate activity in ng/ml and [DHT] indicates activity in nM.
[mCherry] = c_1 + c_2 [Testosterone]^2
where square brackets indicate activity in M, and c_1 and c_2 are constants to be determined.
Note: This model is accurate only when [DHT] is less than 50 nM.
Click Here For More Info
Promoter Selection Model
Click Here For More Info
References
- Shicheng Liu, Hitoshi Yamauchi. (2008). "Different patterns of 5-alpha-reductase expression, cellular distribution, and testosterone metamolism in human follicular dermal papilla cells." Biochemical and Biophysical Research Communications 368 (2008) 858–864
- Jane Yuxia Qin (2010). "Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter." PLOS ONE. May 12, 2010. https://doi.org/10.1371/journal.pone.0010611
- Meehan KL1, Sadar MD. (2003). "Androgens and androgen receptor in prostate and ovarian malignancies." Front Biosci. 2003 May 1;8:d780-800.