Difference between revisions of "Team:NYMU-Taipei/experimentsandresults"

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<h1 id="2" class="subtitle">Protocols</h1>
 
<h1 id="2" class="subtitle">Protocols</h1>
 
<br>
 
<br>
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<h2>E.coli Protocols</h2>
 
<div class="protocols">
 
<div class="protocols">
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<div id="1t" class="trigger"onclick="detail(event)">Plasmid Extraction
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<div id="1t" class="trigger"onclick="detail(event)">Two-day Efficient Cloning Cycle
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<div id="1d" class="detail">ballalalla</div></div>
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<div id="1d" class="detail">
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<p>We used an efficient two day cloning cycle split into a "Light" day and a "Heavy" day.</p>
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<h3>Light Day</h3>
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<p>The light day consists of Colony PCR and liquid culture of colonies transformed from a previous day.</p>
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<ol>
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<li>
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The 3-in-1
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<p>First, count the number of colonies you want to check. Then, do the following 3 things sequentially:</p>
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<ul>
 +
<li>Liquid culture</li>
 +
<li>2nd time plate</li>
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<li>Colony PCR</li>
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</ul>
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<p>(Use the same tip to add the template to these three things)</p>
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</li>
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<li>Make The Gel For Electrophoresis</li>
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<li>Run Gel Electrophoresis To Check The Colony PCR Product</li>
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</ol>
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</div></div>
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<div id="2t" class="trigger"onclick="detail(event)">Plasmid Extraction
 
<div id="2t" class="trigger"onclick="detail(event)">Plasmid Extraction
 
<div id="2d" class="detail">ballalalla</div></div>
 
<div id="2d" class="detail">ballalalla</div></div>

Revision as of 09:43, 29 September 2018

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Protocols


E.coli Protocols

Two-day Efficient Cloning Cycle

We used an efficient two day cloning cycle split into a "Light" day and a "Heavy" day.

Light Day

The light day consists of Colony PCR and liquid culture of colonies transformed from a previous day.

  1. The 3-in-1

    First, count the number of colonies you want to check. Then, do the following 3 things sequentially:

    • Liquid culture
    • 2nd time plate
    • Colony PCR

    (Use the same tip to add the template to these three things)

  2. Make The Gel For Electrophoresis
  3. Run Gel Electrophoresis To Check The Colony PCR Product
Plasmid Extraction
ballalalla
Plasmid Extraction
ballalalla
Plasmid Extraction
ballalalla