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Revision as of 03:44, 1 October 2018
HAIR TO STAY
Protocols
E.coli Protocols
Two-day Efficient Cloning Cycle
We used an efficient two day cloning cycle split into a "Light" day and a "Heavy" day.
Light Day
The light day consists of Colony PCR and liquid culture of colonies transformed from a previous day.
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The 3-in-1
First, count the number of colonies you want to check. Then, do the following 3 things sequentially:
- Liquid culture
- 2nd time plate
- Colony PCR
(Use the same tip to add the template to these three things)
- Make The Gel For Electrophoresis
- Run Gel Electrophoresis To Check The Colony PCR Product
Plasmid Extraction
ballalalla
Plasmid Extraction
ballalalla
Plasmid Extraction
ballalalla