Difference between revisions of "Team:NTHU Formosa/InterLab"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>InterLab</h1>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
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For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab">InterLab study</a>, all work must be shown on this page.
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    <h2 class="w3-wide">IGEM 2018 Interlab Study</h2>
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    <h3 class="w3-wide">Overall To-Do List </h3>
  
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Calibration 1：​​ Reference point - LUDOX Protocol
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Record settings for standard measurements
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Record measurements
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Fill out corresponding Excel sheet
  
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Calibration 2：​Particle Standard Curve - Microsphere Protocol
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Prepare Microsphere Stock Solution
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Serial dilution
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Measure Abs600 of all samples in instrument
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Record measurements
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Fill out corresponding Excel sheet
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Calibration 3：Fluorescence standard curve - Fluorescein Protocol
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Resuspend and dilute fluorescein
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Serial dilutions
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Measure fluorescence of all samples in instrument
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Record measurements
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Fill out corresponding Excel sheet
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Cell Measurement protocol
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Day 1: Transform E.Coli DH5α with plasmids (all in pSB1C3)
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Day 2: pick 2 colonies per plate (5 mL LB medium + Chloramphenicol. Grow 16-18 hours @ 37 C and 220 rpm
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Day 3:
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Dilution cultures in LB+Chloramphenicol
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Measure
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Further dilute
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Incubate
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Abs600 and fluorescence measurement
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Record measurements
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Fill out corresponding Excel sheet
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Protocol：Colony Forming Units per 0.1  E. coli cultures</p>
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Revision as of 10:51, 2 October 2018

IGEM 2018 Interlab Study

Overall To-Do List

Calibration 1：​​ Reference point - LUDOX Protocol Record settings for standard measurements Record measurements Fill out corresponding Excel sheet Calibration 2：​Particle Standard Curve - Microsphere Protocol Prepare Microsphere Stock Solution Serial dilution Measure Abs600 of all samples in instrument Record measurements Fill out corresponding Excel sheet Calibration 3：Fluorescence standard curve - Fluorescein Protocol Resuspend and dilute fluorescein Serial dilutions Measure fluorescence of all samples in instrument Record measurements Fill out corresponding Excel sheet Cell Measurement protocol Day 1: Transform E.Coli DH5α with plasmids (all in pSB1C3) Day 2: pick 2 colonies per plate (5 mL LB medium + Chloramphenicol. Grow 16-18 hours @ 37 C and 220 rpm Day 3: Dilution cultures in LB+Chloramphenicol Measure Further dilute Incubate Abs600 and fluorescence measurement Record measurements Fill out corresponding Excel sheet Protocol：Colony Forming Units per 0.1 E. coli cultures