Difference between revisions of "Team:NTHU Formosa/Collaborations"

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{{NTHU_Formosa}}
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{{NTHU_Formosa}} {{NTHU_Formosa/NavBar}}
 
<html>
 
<html>
  
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<head>
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  <link rel="stylesheet" href="https://www.w3schools.com/w3css/4/w3.css">
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  <link rel="stylesheet" href="https://fonts.googleapis.com/css?family=Lato">
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  <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css">
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  <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/CSS&action=raw&ctype=text/css">
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  <style>
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    /* unvisited link */
  
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    a {
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      color: #0645AD;
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    }
  
<div class="column full_size judges-will-not-evaluate">
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    /* visited link */
<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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</div>
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    a:visited {
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      color: green;
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    }
  
<div class="clear"></div>
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      color: hotpink;
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      cursor: pointer;
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<div class="column full_size">
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    /* selected link */
<h1>Collaborations</h1>
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<p>
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    a:active {
Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
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      color: blue;
</p>
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  </style>
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</head>
  
<h3>Silver Medal Criterion #2</h3>
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<!-- Main Section -->
<p>
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<div class="w3-container w3-pale-blue w3-padding-64 w3-center">
Complete this page if you intend to compete for the silver medal criterion #2 on collaboration. Please see the <a href="https://2018.igem.org/Judging/Medals">2018 Medals Page</a> for more information.
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  <div class="w3-content">
</p>
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  <p class="w3-center" style="font-size:60px;">Team Collaboration</p>
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<br><br>
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    <p class="w3-center"> We had several meetings with 2018 iGEM team NTHU_Taiwan since August 3rd to troubleshoot their problem on making constructs.
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<br><br>
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Earlier before the meeting, NTHU_Taiwan designed the gene of insert for their experiment. After getting the synthesized gene part from IDT and digesting both the inserts and the vectors, they proceeded to ligation, which failed after times they tried. <br><br>
 +
 
 +
During the discussion, the design of the gene, cutting site and many other aspects are checked by us and appeared to be just fine. Later we concluded that the failing of ligation is due to low concentration of the inserts left following digestion and purification.<br><br>
 +
 
 +
To avoid the digestion and purification steps, which caused the loss of their inserts, we re-designed primers for them and suggested NEBuilder Assembly kit for ligation. The kit requires lower DNA input and can save time by avoiding PCR amplification steps. Gladly, team NTHU_Taiwan successfully made all three constructs they needed-- pSB1C3 - AHL snesor - Anti – STAR, pSB1C3 - pLac – STAR, pSB1C3 - pLux – GFP—following our instructions.2018.08.03</p>
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<br><br>
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  </div>
 
</div>
 
</div>
  
<div class="column two_thirds_size">
 
  
<h4> Which other teams can we work with? </h4>
 
<p>
 
You can work with any other team in the competition, including software, hardware, high school and other tracks. You can also work with non-iGEM research groups, but they do not count towards the iGEM team collaboration silver medal criterion.
 
</p>
 
  
<p>
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<div class="w3-container w3-pale-blue w3-padding-64 w3-center">
In order to meet the silver medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
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  <div class="w3-content">
</p>
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<p class="w3-center" style="font-size:40px;">Wiki Page</p>
  
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<p class="w3-center" style="font-size:20px;">Since building wiki pages is unfamiliar to our team, on August 24th , team NCTU­_Formosa gave us a tutorial about html.</p><br>
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    <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/f/fe/T--NTHU_Formosa--NCTU01.jpg" align=left style="width:55%;margin:20px 20px;box-shadow:3px 3px 12px gray;";>
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<p class="w3-justify" style="font-size:20px;">During the tutorial, we learned:</p>
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<p class="w3-justify" style="font-size:15px;">1.Basic tags and elements for html</p>
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<p class="w3-justify" style="font-size:15px;">2.Image scale and website format adjustment</p>
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<p class="w3-justify" style="font-size:15px;">3.Styling html with CSS and JavaScript</p>
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<p class="w3-justify" style="font-size:15px;">4.Conversion of websites in PC and mobile version</p><br>
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<br><br><br><br>
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<p class="w3-center" style="font-size:20px;">Thanks to NCTU­_Formosa’s guidance, discussion and their generosity to assist our problems through messeges after the meetup, the wiki page we expected can be built successfully by a team composing of biology background. We both learned a lot from each other.</p><br>
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  </div>
 
</div>
 
</div>
  
  
  
<div class="column third_size">
 
<p>
 
Here are some suggestions for projects you could work on with other teams:
 
</p>
 
  
<ul>
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<li> Improve the function of another team's BioBrick Part or Device</li>
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<li> Characterize another team's part </li>
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<li> Debug a construct </li>
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<li> Model or simulate another team's system </li>
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<li> Test another team's software</li>
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<li> Help build and test another team's hardware project</li>
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<li> Mentor a high-school team</li>
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</ul>
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<!-- Trigger/Open The Modal -->
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<!-- <a id="myLink"><u>Open Modal</u></a> -->
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<!-- The Modal Box-->
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<div id="myModal" class="modal">
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  <!-- Modal content -->
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  <div class="modal-content">
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    <span class="close">&times;</span>
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    <p>Description text in the box. Image can also be included</p>
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  </div>
 
</div>
 
</div>
  
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<!-- Modal script, DO NOT MODIFY!!! -->
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<script>
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  // Get the modal
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  var modal = document.getElementById('myModal');
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  // Get the hyperlink
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  var link = document.getElementById("myLink");
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  // // When the user clicks the hyperlink, open the modal
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  link.onclick = function() {
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    modal.style.display = "block";
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  }
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  // Get the <span> element that closes the modal
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  var span = document.getElementsByClassName("close")[0];
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  // When the user clicks on <span> (x), close the modal
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  span.onclick = function() {
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    modal.style.display = "none";
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  }
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  // When the user clicks anywhere outside of the modal, close it
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  window.onclick = function(event) {
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    if (event.target == modal) {
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      modal.style.display = "none";
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    }
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  }
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</script>
  
 
</html>
 
</html>

Revision as of 10:52, 2 October 2018

Team Collaboration



We had several meetings with 2018 iGEM team NTHU_Taiwan since August 3rd to troubleshoot their problem on making constructs.

Earlier before the meeting, NTHU_Taiwan designed the gene of insert for their experiment. After getting the synthesized gene part from IDT and digesting both the inserts and the vectors, they proceeded to ligation, which failed after times they tried.

During the discussion, the design of the gene, cutting site and many other aspects are checked by us and appeared to be just fine. Later we concluded that the failing of ligation is due to low concentration of the inserts left following digestion and purification.

To avoid the digestion and purification steps, which caused the loss of their inserts, we re-designed primers for them and suggested NEBuilder Assembly kit for ligation. The kit requires lower DNA input and can save time by avoiding PCR amplification steps. Gladly, team NTHU_Taiwan successfully made all three constructs they needed-- pSB1C3 - AHL snesor - Anti – STAR, pSB1C3 - pLac – STAR, pSB1C3 - pLux – GFP—following our instructions.2018.08.03



Wiki Page

Since building wiki pages is unfamiliar to our team, on August 24th , team NCTU­_Formosa gave us a tutorial about html.


During the tutorial, we learned:

1.Basic tags and elements for html

2.Image scale and website format adjustment

3.Styling html with CSS and JavaScript

4.Conversion of websites in PC and mobile version






Thanks to NCTU­_Formosa’s guidance, discussion and their generosity to assist our problems through messeges after the meetup, the wiki page we expected can be built successfully by a team composing of biology background. We both learned a lot from each other.