Difference between revisions of "Team:NTHU Formosa/InterLab"
| Line 7: | Line 7: | ||
<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css"> | <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css"> | ||
<link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/CSS&action=raw&ctype=text/css"> | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/CSS&action=raw&ctype=text/css"> | ||
| + | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/initial&action=raw&ctype=text/css"> | ||
<style> | <style> | ||
/* unvisited link */ | /* unvisited link */ | ||
Revision as of 12:20, 4 October 2018
IGEM 2018 Interlab Study
Overall To-Do List
Calibration 1：OD600 Reference point - LUDOX Protocol
1.Record settings for standard measurements
2.Record measurements
3.Fill out corresponding Excel sheet
Calibration 2：Particle Standard Curve - Microsphere Protocol
1.Prepare Microsphere Stock Solution
2.Serial dilution
3.Measure Abs600 of all samples in instrument
4.Record measurements
5.Fill out corresponding Excel sheet
Calibration 3：Fluorescence standard curve - Fluorescein Protocol
1.Resuspend and dilute fluorescein
2.Serial dilutions
3.Measure fluorescence of all samples in instrument
4.Record measurements
5.Fill out corresponding Excel sheet
Cell Measurement protocol
Day 1:
Transform E.Coli DH5α with plasmids (all in pSB1C3)
Day 2:
pick 2 colonies per plate (5 mL LB medium + Chloramphenicol. Grow 16-18 hours @ 37 C and 220 rpm
Day 3:
Dilution cultures in LB+Chloramphenicol
Measure OD600
Further dilute
Incubate
Abs600 and fluorescence measurement
Record measurements
Fill out corresponding Excel sheet
Protocol：Colony Forming Units per 0.1 OD600 E.coli cultures