Difference between revisions of "Team:BioMarvel/Notebook/Lab Journal"

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<p>contents</p>
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<h1>July</h1>
 +
<div class="mNoteBookJH"><h3>7/9/2018 </h3></div>
 +
<div class="mNoteBookJ">
 +
<p>Measurement of Abs 600 and OD 600 for LUDOX CL-Xand water</p>
 +
<p>Prepared a dilution series of silica microspheres and measure the Abs 600 in a plate reader</p>
 +
<p>Prepared a dilution series of fluorescein in four replicates and measure the fluorescence</p>
 +
</div>
 +
<div class="mNoteBookJH"><h3>7/10/2018 – 7/22/2018</h3></div>
 +
<div class="mNoteBookJ">
 +
<p>Transform <i>E. coli</i> DH5α with InterLab study plasmids</p>
 +
<p>Pick colonies from each of the transformation plates and inoculate in 5-10 mL LB medium +</p>
 +
<p>Chloramphenicol. Grow the cells overnight. Cell growth, sampling, and assay</p>
 +
<p style="color: red;">Repeat experiments were conducted to confirm the reproducibility of the data</p>
 +
</div>
 +
<div class="mNoteBookJH"><h3>7/23/2018 (Composite parts cloning started)</h3></div>
 +
<div class="mNoteBookJ">
 +
<p>DNA fragments werereceivedfrom Integrated DNA Technologies (IDT)</p>
 +
</div>
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<div class="mNoteBookJH"><h3>7/24/2018 – 7/31/2018</h3></div>
 +
<div class="mNoteBookJ">
 +
<p>Conducted A-tailing process at the end of our gene fragment for TA cloning</p>
 +
<p>A-tailed gene fragment was ligated with the TA vector</p>
 +
<p>Transformed<i>E. coli</i> DH5α with ligated TA vector (Blue-white screen)</p>
 +
<p>Picked colonies from transformation plates. Cells were grown in LB broth with 0.1mM of ampicillin</p>
 +
<p>Vector isolation was conducted through DNA mini prep kit</p>
 +
<p>DNA fragment parts were purified using gel electrophoresis and ligated with pSB1C3 vector.</p>
 +
<p>Checked appropriate insertion of the construct in the vector </p>
 +
<br>
 +
<p style="color: red;">Repeat experiments were conducted for debugging</p>
 +
</div>
 +
</section>
 +
<section id="mSection">
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<h1>August</h1>
 +
<div class="mNoteBookJH"><h3>8/1/2018 – 8/11/2018</h3></div>
 +
<div class="mNoteBookJ">
 +
<p>Plasmid sequence was confirmed by BIOFACT™ sequencing analysis service</p>
 +
<p>Amplification and purification of GBP-ProG fusion Protein</p>
 +
<p>Purified proteins were confirmed by SDS-PAGE</p>
 +
<p>Protein quantified using the Bradford protein assay</p>
 +
</div>
 +
<div class="mNoteBookJH"><h3>8/12/2018 – 8/19/2018</h3></div>
 +
<div class="mNoteBookJ">
 +
<p>Fabrication of interdigitated array (IDA) gold electrode</p>
 +
<p>Fabricatedelectrochemical immunochip</p>
 +
<p>Cyclic voltammograms were measured for electrochemical detection</p>
 +
<p style="color: red;">Repeat experiments were conducted to confirm the reproducibility of the data</p>
 +
</div>
 +
</section>
 +
<section id="mSection">
 +
<h1>September</h1>
 +
<div class="mNoteBookJH"><h3>September</h3></div>
 +
<div class="mNoteBookJ">
 +
<p>PCR primers werereceivedfrom BIOFACT™</p>
 +
</div>
 +
<div class="mNoteBookJH"><h3>9/11/2018 – 9/22/2018</h3></div>
 +
<div class="mNoteBookJ">
 +
<p>A series of experiments was the same as a composite parts cloning experiment.</p>
 +
<p>Conducted cloning experiments for two basic parts (GBP, ProG)</p>
 +
<p>Plasmid sequence was confirmed by BIOFACT™ sequencing analysis service</p>
 +
<br>
 +
<p style="color: red;">Repeat experiments were conducted for debugging</p>
 +
</div>
 +
</section>
 
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Revision as of 07:11, 6 October 2018

≡
　

July

7/9/2018

Measurement of Abs 600 and OD 600 for LUDOX CL-Xand water

Prepared a dilution series of silica microspheres and measure the Abs 600 in a plate reader

Prepared a dilution series of fluorescein in four replicates and measure the fluorescence

7/10/2018 – 7/22/2018

Transform E. coli DH5α with InterLab study plasmids

Pick colonies from each of the transformation plates and inoculate in 5-10 mL LB medium +

Chloramphenicol. Grow the cells overnight. Cell growth, sampling, and assay

Repeat experiments were conducted to confirm the reproducibility of the data

7/23/2018 (Composite parts cloning started)

DNA fragments werereceivedfrom Integrated DNA Technologies (IDT)

7/24/2018 – 7/31/2018

Conducted A-tailing process at the end of our gene fragment for TA cloning

A-tailed gene fragment was ligated with the TA vector

TransformedE. coli DH5α with ligated TA vector (Blue-white screen)

Picked colonies from transformation plates. Cells were grown in LB broth with 0.1mM of ampicillin

Vector isolation was conducted through DNA mini prep kit

DNA fragment parts were purified using gel electrophoresis and ligated with pSB1C3 vector.

Checked appropriate insertion of the construct in the vector


Repeat experiments were conducted for debugging

August

8/1/2018 – 8/11/2018

Plasmid sequence was confirmed by BIOFACT™ sequencing analysis service

Amplification and purification of GBP-ProG fusion Protein

Purified proteins were confirmed by SDS-PAGE

Protein quantified using the Bradford protein assay

8/12/2018 – 8/19/2018

Fabrication of interdigitated array (IDA) gold electrode

Fabricatedelectrochemical immunochip

Cyclic voltammograms were measured for electrochemical detection

Repeat experiments were conducted to confirm the reproducibility of the data

September

September

PCR primers werereceivedfrom BIOFACT™

9/11/2018 – 9/22/2018

A series of experiments was the same as a composite parts cloning experiment.

Conducted cloning experiments for two basic parts (GBP, ProG)

Plasmid sequence was confirmed by BIOFACT™ sequencing analysis service


Repeat experiments were conducted for debugging

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