Difference between revisions of "Team:BioMarvel/Notebook/Lab Journal"
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<h1>July</h1> | <h1>July</h1> | ||
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내용 | 내용 | ||
<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | ||
| − | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/9/2018</h4></a>< | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/9/2018</h4></a><DIVclass="mNoteBookJ" style='display:none'> |
<p>Measurement of Abs 600 and OD 600 for LUDOX CL-Xand water</p> | <p>Measurement of Abs 600 and OD 600 for LUDOX CL-Xand water</p> | ||
<p>Prepared a dilution series of silica microspheres and measure the Abs 600 in a plate reader</p> | <p>Prepared a dilution series of silica microspheres and measure the Abs 600 in a plate reader</p> | ||
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<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A> | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A> | ||
</DIV> | </DIV> | ||
| − | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/10/2018 – 7/22/2018</h4></a><DIV class="mNoteBookJ" style='display:none'> | |
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<p>Transform <i>E. coli</i> DH5α with InterLab study plasmids</p> | <p>Transform <i>E. coli</i> DH5α with InterLab study plasmids</p> | ||
<p>Pick colonies from each of the transformation plates and inoculate in 5-10 mL LB medium +</p> | <p>Pick colonies from each of the transformation plates and inoculate in 5-10 mL LB medium +</p> | ||
<p>Chloramphenicol. Grow the cells overnight. Cell growth, sampling, and assay</p> | <p>Chloramphenicol. Grow the cells overnight. Cell growth, sampling, and assay</p> | ||
<p style="color: red;">Repeat experiments were conducted to confirm the reproducibility of the data</p> | <p style="color: red;">Repeat experiments were conducted to confirm the reproducibility of the data</p> | ||
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| − | < | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/23/2018 (Composite parts cloning started)</h4></a><DIV style='display:none'> |
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<p>DNA fragments werereceivedfrom Integrated DNA Technologies (IDT)</p> | <p>DNA fragments werereceivedfrom Integrated DNA Technologies (IDT)</p> | ||
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| − | < | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/24/2018 – 7/31/2018</h4></a><DIV class="mNoteBookJ" style='display:none'> |
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<p>Conducted A-tailing process at the end of our gene fragment for TA cloning</p> | <p>Conducted A-tailing process at the end of our gene fragment for TA cloning</p> | ||
<p>A-tailed gene fragment was ligated with the TA vector</p> | <p>A-tailed gene fragment was ligated with the TA vector</p> | ||
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<p style="color: red;">Repeat experiments were conducted for debugging</p> | <p style="color: red;">Repeat experiments were conducted for debugging</p> | ||
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</section> | </section> | ||
<section id="mSection"> | <section id="mSection"> | ||
<h1>August</h1> | <h1>August</h1> | ||
| − | < | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>8/1/2018 – 8/11/2018</h4></a><DIV class="mNoteBookJ" style='display:none'> |
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<p>Plasmid sequence was confirmed by BIOFACT™ sequencing analysis service</p> | <p>Plasmid sequence was confirmed by BIOFACT™ sequencing analysis service</p> | ||
<p>Amplification and purification of GBP-ProG fusion Protein</p> | <p>Amplification and purification of GBP-ProG fusion Protein</p> | ||
<p>Purified proteins were confirmed by SDS-PAGE</p> | <p>Purified proteins were confirmed by SDS-PAGE</p> | ||
<p>Protein quantified using the Bradford protein assay</p> | <p>Protein quantified using the Bradford protein assay</p> | ||
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| − | < | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>8/12/2018 – 8/19/2018</h4></a><DIV class="mNoteBookJ" style='display:none'> |
| − | + | <p>Plasmid sequence was confirmed by BIOFACT™ sequencing analysis service</p> | |
| − | <p> | + | <p>Amplification and purification of GBP-ProG fusion Protein</p> |
| − | <p> | + | <p>Purified proteins were confirmed by SDS-PAGE</p> |
| − | <p> | + | <p>Protein quantified using the Bradford protein assay</p> |
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</section> | </section> | ||
<section id="mSection"> | <section id="mSection"> | ||
<h1>September</h1> | <h1>September</h1> | ||
| − | < | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>9/10/2018 (Basic parts cloning started)</h4></a><DIV class="mNoteBookJ" style='display:none'> |
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<p>PCR primers werereceivedfrom BIOFACT™</p> | <p>PCR primers werereceivedfrom BIOFACT™</p> | ||
| − | </ | + | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> |
| − | < | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>9/11/2018 – 9/22/2018</h4></a><DIV class="mNoteBookJ" style='display:none'> |
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<p>A series of experiments was the same as a composite parts cloning experiment.</p> | <p>A series of experiments was the same as a composite parts cloning experiment.</p> | ||
<p>Conducted cloning experiments for two basic parts (GBP, ProG)</p> | <p>Conducted cloning experiments for two basic parts (GBP, ProG)</p> | ||
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<p style="color: red;">Repeat experiments were conducted for debugging</p> | <p style="color: red;">Repeat experiments were conducted for debugging</p> | ||
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</section> | </section> | ||
</div> | </div> | ||
Revision as of 08:03, 6 October 2018
7/10/2018 – 7/22/2018
7/23/2018 (Composite parts cloning started)
7/24/2018 – 7/31/2018
August
8/1/2018 – 8/11/2018
8/12/2018 – 8/19/2018
September
9/10/2018 (Basic parts cloning started)
9/11/2018 – 9/22/2018
Copyright © 2018 By BioMarvel. All right reserved.