(Prototype team page) |
Cyclohexane (Talk | contribs) |
||
Line 1: | Line 1: | ||
− | + | <html lang="en"> | |
− | <html> | + | |
+ | <head> | ||
+ | <meta charset="UTF-8" name="viewport" content="width=device-width, initial-scale=1.0, maximum-scale=1.0, user-scalable=0"> | ||
+ | <meta http-equiv="X-UA-Compatible" content="IE=edge"> | ||
+ | <!--ie使用edge渲染模式--> | ||
+ | <meta content="width=device-width,initial-scale=1.0,maximum-scale=1.0,user-scalable=no" id="viewport" name="viewport"> | ||
+ | <meta name="renderer" content="webkit"> | ||
+ | <!--360渲染模式--> | ||
+ | <meta name="format-detection" content="telephone=notelphone=no, email=no" /> | ||
+ | <meta name="description" content="" /> | ||
+ | <meta name="keywords" content="" /> | ||
+ | <link rel="shortcut icon" href="favicon.ico" type="image/x-icon" /> | ||
+ | <meta name="apple-touch-fullscreen" content="yes" /><!-- 是否启用 WebApp 全屏模式,删除苹果默认的工具栏和菜单栏 --> | ||
+ | <meta name="apple-mobile-web-app-status-bar-style" content="black" /><!-- 设置苹果工具栏颜色:默认值为 default,可以定为 black和 black-translucent--> | ||
+ | <meta http-equiv="Cache-Control" content="no-siteapp" /><!-- 不让百度转码 --> | ||
+ | <meta name="HandheldFriendly" content="true"><!-- 针对手持设备优化,主要是针对一些老的不识别viewport的浏览器,比如黑莓 --> | ||
+ | <meta name="MobileOptimized" content="320"><!-- 微软的老式浏览器 --> | ||
+ | <meta name="screen-orientation" content="portrait"><!-- uc强制竖屏 --> | ||
+ | <meta name="x5-orientation" content="portrait"><!-- QQ强制竖屏 --> | ||
+ | <meta name="browsermode" content="application"><!-- UC应用模式 --> | ||
+ | <meta name="x5-page-mode" content="app"><!-- QQ应用模式 --> | ||
+ | <meta name="msapplication-tap-highlight" content="no"><!-- windows phone 点击无高光 --> | ||
+ | <title>Team:XMU-China/Description - 2018.igem.org</title> | ||
− | |||
− | < | + | <link rel="stylesheet" href="css/safety.css"> |
− | < | + | |
+ | <link href="http://cdn.bootcss.com/font-awesome/4.7.0/css/font-awesome.min.css" rel="stylesheet"> | ||
+ | <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/cover?action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/footer?action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/nav?action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/nav_mobile?action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/safety?action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/font?action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/material-scrolltop?action=raw&ctype=text/css"> | ||
+ | </head> | ||
− | < | + | <body> |
− | + | <div id="container"> | |
− | </ | + | <header> |
− | + | <div class="wrapper cf"> | |
− | + | <nav id="main-nav"> | |
− | < | + | <ul class="first-nav"> |
− | < | + | <li class="Project"> |
− | + | <a href="#" target="_blank">Project</a> | |
− | < | + | <ul> |
− | + | <li><a href="https://2018.igem.org/Team:XMU-China/Description">Description</a></li> | |
− | <ul> | + | <li><a href="https://2018.igem.org/Team:XMU-China/Design">Design</a></li> |
− | <li> | + | <li><a href="https://2018.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li> |
− | <li> | + | <li><a href="https://2018.igem.org/Team:XMU-China/Results">Results</a></li> |
− | <li> | + | <li><a href="https://2018.igem.org/Team:XMU-China/Parts">Parts</a></li> |
− | <li> | + | </ul> |
− | </ul> | + | </li> |
− | + | </ul> | |
− | </div> | + | <ul class="second-nav"> |
− | + | <li class="Hardware"> | |
− | <div class=" | + | <a href="#">Hardware</a> |
− | < | + | <ul> |
− | + | <li><a href="https://2018.igem.org/Team:XMU-China/Hardware">Overview</a></li> | |
− | < | + | <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Microfluidic_Chips">Microfluidic chips</a></li> |
− | + | <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Fluorescenc_Detection">Fluorescence Detection</a></li> | |
− | + | <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Straberry_Pi">Straberry Pi</a></li> | |
− | < | + | <li><a href="https://2018.igem.org/Team:XMU-China/Applied_Design">Applied Design</a></li> |
− | + | <li><a href="https://2018.igem.org/Team:XMU-China/Software">APP</a></li> | |
− | <p> | + | </ul> |
− | </div> | + | </li> |
+ | <li class="Model"> | ||
+ | <a href="#">Model</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Model">Overview</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="Human_Practice"> | ||
+ | <a href="#">Human Practice</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Te | ||
+ | am:XMU-China/Human_Practices">Overview</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/HP/Silver">Silver</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/HP/Gold_Integrated">Gold</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Public_Engagement">Engagement</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Entrepreneurship">Entrepreneurship</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="Other_Works"> | ||
+ | <a href="#">Other Works</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/InterLab">InterLab</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Improve">Improve</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Safety">Safety</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Measurement">Measurement</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="Notebook"> | ||
+ | <a href="#">Notebook</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Notebook">Notebook</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Experiments">Experiments</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="Team"> | ||
+ | <a href="#">Team</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Team">Members</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Attributions">Attributions</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Judging">Judging</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </nav> | ||
+ | <a class="toggle"><span></span></a> | ||
+ | </div> | ||
+ | </header> | ||
+ | </div> | ||
+ | <script src="js/jquery-1.11.0.min.js"></script> | ||
+ | <!-- <script src="js/hc-mobile-nav.js"></script> --> | ||
+ | <script src="https://2018.igem.org/Team:XMU-China/js/hc-mobile-nav?action=raw&ctype=text/javascript"></script> | ||
+ | <div class="header"> | ||
+ | <div class="logo"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/b/b5/T--XMU-China--singlelogo.png"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/3/35/T--XMU-China--iGEM_logo.png"> | ||
+ | </div> | ||
+ | <div class="clear"></div> | ||
+ | <div class="nav"> | ||
+ | <div id="Team"> | ||
+ | <div class="nav-word">Team</div> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Team">Members</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Attributions">Attributions</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Judging">Judging</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div id="Notebook"> | ||
+ | <div> | ||
+ | <div class="nav-word">Notebook</a></div> | ||
+ | </div> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Notebook">Notebook</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Experiments">Experiments</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div id="Other_Works"> | ||
+ | <div class="nav-word">Other Works</div> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/InterLab">InterLab</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Improve">Improve</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Safety">Safety</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Measurement">Measurement</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div id="Human_Practice"> | ||
+ | <div class="nav-word">Human Practice</div> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Human_Practices">Overview</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/HP/Silver">Silver</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/HP/Gold_Integrated">Gold</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Public_Engagement">Engagement</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Entrepreneurship">Entrepreneurship</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div id="Model"> | ||
+ | <div class="nav-word">Model</div> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Model">Overview</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div id="Hardwork"> | ||
+ | <div class="nav-word">Hardware</div> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Hardware">Overview</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Microfluidic_Chips">Microfluidic chips</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Fluorescenc_Detection">Fluorescence Detection</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Straberry_Pi">Straberry Pi</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Applied_Design">Applied Design</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Software">APP</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div id="Project"> | ||
+ | <div class="nav-word">Project</div> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Description">Description</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Design">Design</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Results">Results</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:XMU-China/Parts">Parts</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div id="jiannan"> | ||
+ | <a href="https://2018.igem.org/Team:XMU-China"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/6/6f/T--XMU-China--jiannanlogo.png"> | ||
+ | </a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="clear"></div> | ||
+ | <div class="description_banner"> | ||
+ | <div class="word">Description</div> | ||
+ | </div> | ||
+ | <nav class="Quick-navigation"> | ||
+ | <div class="Quick-navigation_word"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/f/f5/T--XMU-China--right0.png"> | ||
+ | <a href="#Safe_Project_Design" class="Quick-navigation-item"> | ||
+ | <img id="turn_img" src="https://static.igem.org/mediawiki/2018/d/db/T--XMU-China--right1.png"> | ||
+ | <a href="#Safe_Project_Design"id="Quick_A">Safe Project Design</a></a> | ||
+ | <a href="#Safe_Lab_Work" class="Quick-navigation-item" > | ||
+ | <img id="turn_img" src="https://static.igem.org/mediawiki/2018/c/cd/T--XMU-China--right2.png"> | ||
+ | <a href="#Safe_Lab_Work" id="Quick_B">Safe Lab Work</a></a> | ||
+ | <a href="#Safe_Shipment" class="Quick-navigation-item"> | ||
+ | <img id="turn_img" src="https://static.igem.org/mediawiki/2018/1/1c/T--XMU-China--right3.png"> | ||
+ | <a href="#Safe_Shipment" id="Quick_C">Safe Shipment</a></a> | ||
+ | </div> | ||
+ | </nav> | ||
+ | <div class="main"> | ||
+ | <section id="Safe_Project_Design" class="js-scroll-step"> | ||
+ | <div class="headline"> | ||
+ | Safe Project Design | ||
+ | </div> | ||
+ | <P>Firstly, we use E.coli as our chassis organisms and do not use any other organism. E.coli DH5α, BL21 and BL21(DE3) were the only chassis when we performed the molecular cloning and expression. All the plasmids are harmless for humans and none of our parts would raise any safety issue according to the current expertise. In order to overcome the deficiencies of traditional detection methods, we have developed an Aptamer Based Cell-free Detection system of protein (ABCD system). We used the aptamer SYL3C against EpCAM, an epithelial cell adhesion molecule that is highly expressed on the surface of adenocarcinoma cells. After protein detection, we use outer-membrane vesicles (OMVs) to treat the diseased cells. For the sake of allowing our detection and treatment system to function in reality, we designed KaiABC system and TDPs system to regulate the expression rate of OMVs and store fragile chemicals or biological materials.</P> | ||
+ | </section> | ||
+ | <section id="Safe_Lab_Work" class="js-scroll-step"> | ||
+ | <div class="headline"> | ||
+ | Safe Lab Work | ||
+ | </div> | ||
+ | <p>The building of laboratory include the location of emergency exits, emergency ventilation system, fire-fighting equipment, first aid kit and so on. Every year after recruiting new members, we spend a semester training them in basic laboratory operations and handling safety accidents. Laboratory coat and gloves are necessary when entering the laboratory. All culture fluid and culture medium with bacteria were processed properly to prevent the potential biological pollution. We handled all the biological materials, wastes and equipments strictly following the BSL-1 requirements, our lab is also equipped with professional operators of dangerous goods and waste biological products, they will disposal all the waste properly. We turn off the water and electricity, close window and door when we leave the lab. | ||
+ | </p> | ||
+ | |||
+ | </section> | ||
+ | <section id="Safe_Shipment" class="js-scroll-step"> | ||
+ | <div class="headline"> | ||
+ | Supporting | ||
+ | </div> | ||
+ | <P>We always followed iGEM shipment requirements when sending our DNA parts to the Registry.</P> | ||
+ | </section> | ||
+ | </div> | ||
+ | <!-- <script type="text/javascript" src="js/right.js"></script> --> | ||
+ | <script src="https://2018.igem.org/Team:XMU-China/js/right?action=raw&ctype=text/javascript"></script> | ||
+ | <button class="material-scrolltop" type="button"></button> | ||
+ | <script> | ||
+ | window.jQuery || document.write('<script src="js/jquery-1.11.0.min.js"><\/script>') | ||
+ | </script> | ||
+ | <!-- <script src="js/material-scrolltop.js"></script> --> | ||
+ | <script src="https://2018.igem.org/Team:XMU-China/js/material-scrolltop?action=raw&ctype=text/javascript"></script> | ||
+ | <div class="footer"> | ||
+ | <div class="footer_top"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/3/3d/T--XMU-China--xmu_is_different.png"> | ||
+ | <ul> | ||
+ | <li>Home</li> | ||
+ | <li>Basic Part</li> | ||
+ | <li>About us</li> | ||
+ | <li>Composite Part</li> | ||
+ | <li>Attribution</li> | ||
+ | </ul> | ||
+ | <ul> | ||
+ | <li>Design</li> | ||
+ | <li>Modeling</li> | ||
+ | <li>Engagemen</li> | ||
+ | <li>Biosafety</li> | ||
+ | <li>Contribution</li> | ||
+ | </ul> | ||
+ | <ul> | ||
+ | <li>Collaboration</li> | ||
+ | <li>Protocols</li> | ||
+ | <li>Notebook</li> | ||
+ | <li>Description</li> | ||
+ | <li>Experiment</li> | ||
+ | </ul> | ||
+ | <div class="clear"></div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <div class="bottom"></div> | ||
+ | </body> | ||
</html> | </html> |
Revision as of 14:52, 9 October 2018
Firstly, we use E.coli as our chassis organisms and do not use any other organism. E.coli DH5α, BL21 and BL21(DE3) were the only chassis when we performed the molecular cloning and expression. All the plasmids are harmless for humans and none of our parts would raise any safety issue according to the current expertise. In order to overcome the deficiencies of traditional detection methods, we have developed an Aptamer Based Cell-free Detection system of protein (ABCD system). We used the aptamer SYL3C against EpCAM, an epithelial cell adhesion molecule that is highly expressed on the surface of adenocarcinoma cells. After protein detection, we use outer-membrane vesicles (OMVs) to treat the diseased cells. For the sake of allowing our detection and treatment system to function in reality, we designed KaiABC system and TDPs system to regulate the expression rate of OMVs and store fragile chemicals or biological materials.
The building of laboratory include the location of emergency exits, emergency ventilation system, fire-fighting equipment, first aid kit and so on. Every year after recruiting new members, we spend a semester training them in basic laboratory operations and handling safety accidents. Laboratory coat and gloves are necessary when entering the laboratory. All culture fluid and culture medium with bacteria were processed properly to prevent the potential biological pollution. We handled all the biological materials, wastes and equipments strictly following the BSL-1 requirements, our lab is also equipped with professional operators of dangerous goods and waste biological products, they will disposal all the waste properly. We turn off the water and electricity, close window and door when we leave the lab.
We always followed iGEM shipment requirements when sending our DNA parts to the Registry.