Difference between revisions of "Team:NTHU Formosa/InterLab"

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     <p class="w3-center" style="font-size:60px;">IGEM 2018 Interlab Study</p>
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     <p class="w3-center" style="font-size:40px;">IGEM 2018 Interlab Study</p>
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<br>
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<p class="w3-center" style="font-size:40px;">Overall To-Do List</p><br>
 
<p class="w3-center" style="font-size:40px;">Overall To-Do List</p><br>
 
<br>
 
<br>
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     <p class="w3-justify" style="font-size:30px;">Calibration 1：OD<sub>600</sub> Reference point - LUDOX Protocol</p>
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     <p class="w3-justify" style="font-size:25px;padding-left:150px;">Calibration 1：OD<sub>600</sub> Reference point - LUDOX Protocol</p><br>
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     <p class="w3-justify" style="font-size:20px;">1.Record settings for standard measurements</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px;">1.Record settings for standard measurements</p>
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     <p class="w3-justify" style="font-size:20px;">2.Record measurements</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px;">2.Record measurements</p>
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     <p class="w3-justify" style="font-size:20px;">3.Fill out corresponding Excel sheet</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px;">3.Fill out corresponding Excel sheet</p><br>
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     <p class="w3-justify" style="font-size:30px;">Calibration 2：​Particle Standard Curve - Microsphere Protocol</p>
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     <p class="w3-justify" style="font-size:25px;padding-left:150px;">Calibration 2：​Particle Standard Curve - Microsphere Protocol</p><br>
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     <p class="w3-justify" style="font-size:20px;">1.Prepare Microsphere Stock Solution</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px;">1.Prepare Microsphere Stock Solution</p>
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     <p class="w3-justify" style="font-size:20px;">2.Serial dilution</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px;">2.Serial dilution</p>
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     <p class="w3-justify" style="font-size:20px;">3.Measure Abs600 of all samples in instrument</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px;">3.Measure Abs600 of all samples in instrument</p>
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     <p class="w3-justify" style="font-size:20px;">4.Record measurements</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px;">4.Record measurements</p>
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     <p class="w3-justify" style="font-size:20px;">5.Fill out corresponding Excel sheet</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px;">5.Fill out corresponding Excel sheet</p><br>
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     <p class="w3-justify" style="font-size:30px;">Calibration 3：Fluorescence standard curve - Fluorescein Protocol </p>
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     <p class="w3-justify" style="font-size:25px;padding-left:150px;">Calibration 3：Fluorescence standard curve - Fluorescein Protocol </p><br>
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     <p class="w3-justify" style="font-size:20px;">1.Resuspend and dilute fluorescein</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px;">1.Resuspend and dilute fluorescein</p>
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     <p class="w3-justify" style="font-size:20px;">2.Serial dilutions</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px;">2.Serial dilutions</p>
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     <p class="w3-justify" style="font-size:20px;">3.Measure fluorescence of all samples in instrument</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px;">3.Measure fluorescence of all samples in instrument</p>
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     <p class="w3-justify" style="font-size:20px;">4.Record measurements</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px;">4.Record measurements</p>
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     <p class="w3-justify" style="font-size:20px;">5.Fill out corresponding Excel sheet</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px;">5.Fill out corresponding Excel sheet</p><br>
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     <p class="w3-justify" style="font-size:40px;">Cell Measurement protocol</p>
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     <p class="w3-justify" style="font-size:25px;padding-left:150px;">Cell Measurement protocol</p><br>
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     <p class="w3-justify" style="font-size:25px;">Day 1:</p>
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     <p class="w3-justify" style="font-size:25px;padding-left:150px;">Day 1:</p><br>
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     <p class="w3-justify" style="font-size:20px;padding-left:70px">Transform E.Coli DH5α with plasmids (all in pSB1C3)</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px">Transform E.Coli DH5α with plasmids (all in pSB1C3)</p><br>
 
      
 
      
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     <p class="w3-justify" style="font-size:25px;">Day 2:</p>
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     <p class="w3-justify" style="font-size:25px;padding-left:150px;">Day 2:</p><br>
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     <p class="w3-justify" style="font-size:20px;padding-left:70px">pick 2 colonies per plate (5 mL LB medium + Chloramphenicol. Grow 16-18 hours @ 37 C and 220 rpm</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:185px">pick 2 colonies per plate (5 mL LB medium + Chloramphenicol. Grow 16-18 hours @ 37 C and 220 rpm</p><br>
 
      
 
      
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     <p class="w3-justify" style="font-size:25px;">Day 3:</p>
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     <p class="w3-justify" style="font-size:25px;padding-left:150px;">Day 3:</p><br>
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     <p class="w3-justify" style="font-size:20px;padding-left:70px">
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     <p class="w3-justify" style="font-size:20px;padding-left:185px">
 
       Dilution cultures in LB+Chloramphenicol <br>
 
       Dilution cultures in LB+Chloramphenicol <br>
 
       Measure OD<sub>600</sub><br>
 
       Measure OD<sub>600</sub><br>
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     <p class="w3-center" style="font-size:30px;">Protocol：Colony Forming Units per 0.1 OD<sub>600</sub>  E.coli cultures</p>
 
     <p class="w3-center" style="font-size:30px;">Protocol：Colony Forming Units per 0.1 OD<sub>600</sub>  E.coli cultures</p>
 
    
 
    

Revision as of 04:24, 10 October 2018

IGEM 2018 Interlab Study

Overall To-Do List



Calibration 1：OD600 Reference point - LUDOX Protocol


1.Record settings for standard measurements

2.Record measurements

3.Fill out corresponding Excel sheet


Calibration 2：​Particle Standard Curve - Microsphere Protocol


1.Prepare Microsphere Stock Solution

2.Serial dilution

3.Measure Abs600 of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet


Calibration 3：Fluorescence standard curve - Fluorescein Protocol


1.Resuspend and dilute fluorescein

2.Serial dilutions

3.Measure fluorescence of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet


Cell Measurement protocol


Day 1:


Transform E.Coli DH5α with plasmids (all in pSB1C3)


Day 2:


pick 2 colonies per plate (5 mL LB medium + Chloramphenicol. Grow 16-18 hours @ 37 C and 220 rpm


Day 3:


Dilution cultures in LB+Chloramphenicol
Measure OD600
Further dilute
Incubate
Abs600 and fluorescence measurement
Record measurements
Fill out corresponding Excel sheet


Protocol：Colony Forming Units per 0.1 OD600 E.coli cultures