Difference between revisions of "Team:NTHU Formosa/InterLab"

Revision as of 08:32, 10 October 2018

IGEM 2018 Interlab Study

Overall To-Do List

✔ Calibration 1：OD₆₀₀ Reference point - LUDOX Protocol

1.Record settings for standard measurements

2.Record measurements

3.Fill out corresponding Excel sheet

✔ Calibration 2：Particle Standard Curve - Microsphere Protocol

1.Prepare Microsphere Stock Solution

2.Serial dilution

3.Measure Abs600 of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet

✔ Calibration 3：Fluorescence standard curve - Fluorescein Protocol

1.Resuspend and dilute fluorescein

2.Serial dilutions

3.Measure fluorescence of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet

✔ Cell Measurement protocol

Day 1:Transform E.Coli DH5α with plasmids (all in pSB1C3)

Day 2:pick 2 colonies per plate (5 mL LB medium + Chloramphenicol.

Grow 16-18 hours @ 37 C and 220 rpm

Day 3: Dilution cultures in LB+Chloramphenicol

Measure OD₆₀₀

Further dilute

Incubate

Abs600 and fluorescence measurement

Record measurements

Fill out corresponding Excel sheet

✔ Protocol：Colony Forming Units per 0.1 OD₆₀₀ E.coli cultures

✔ To see the results, click here.

@@ Line 62: / Line 62: @@
 <p class="w3-center" style="font-size:40px;">Overall To-Do List</p><br>
 <br>
-     <p class="w3-justify" style="font-size:25px;padding-left:150px;">Calibration 1：OD<sub>600</sub> Reference point - LUDOX Protocol</p><br>
+     <p class="w3-justify" style="font-size:25px;padding-left:150px;">✔ Calibration 1：OD<sub>600</sub> Reference point - LUDOX Protocol</p>
+    <p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">1.Record settings for standard measurements</p>
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">2.Record measurements</p>
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">3.Fill out corresponding Excel sheet</p><br>
-     <p class="w3-justify" style="font-size:25px;padding-left:150px;">Calibration 2：Particle Standard Curve - Microsphere Protocol</p><br>
+     <p class="w3-justify" style="font-size:25px;padding-left:150px;">✔ Calibration 2：Particle Standard Curve - Microsphere Protocol</p>
+    <p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">1.Prepare Microsphere Stock Solution</p>
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">2.Serial dilution</p>
@@ Line 72: / Line 78: @@
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">4.Record measurements</p>
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">5.Fill out corresponding Excel sheet</p><br>
-     <p class="w3-justify" style="font-size:25px;padding-left:150px;">Calibration 3：Fluorescence standard curve - Fluorescein Protocol </p><br>
+     <p class="w3-justify" style="font-size:25px;padding-left:150px;">✔ Calibration 3：Fluorescence standard curve - Fluorescein Protocol </p>
+    <p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">1.Resuspend and dilute fluorescein</p>
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">2.Serial dilutions</p>
@@ Line 78: / Line 87: @@
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">4.Record measurements</p>
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">5.Fill out corresponding Excel sheet</p><br>
-     <p class="w3-center" style="font-size:25px;">Cell Measurement protocol</p><br>
+     <p class="w3-justify" style="font-size:25px;padding-left:150px">✔ Cell Measurement protocol</p>
-    <p class="w3-justify" style="font-size:25px;padding-left:180px;">Day 1:</p>
+<p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
-     <p class="w3-justify" style="font-size:20px;padding-left:240px">Transform E.Coli DH5α with plasmids (all in pSB1C3)</p><br>
+     <p class="w3-justify" style="font-size:20px;padding-left:240px">Day 1:Transform E.Coli DH5α with plasmids (all in pSB1C3)</p>
+    <p class="w3-justify" style="font-size:20px;padding-left:240px">Day 2:pick 2 colonies per plate (5 mL LB medium + Chloramphenicol.  </p>
-     <p class="w3-justify" style="font-size:25px;padding-left:180px;">Day 2:</p>
+     <p class="w3-justify" style="font-size:20px;padding-left:296px;">Grow 16-18 hours @ 37 C and 220 rpm</p>
-    <p class="w3-justify" style="font-size:20px;padding-left:240px">pick 2 colonies per plate (5 mL LB medium + Chloramphenicol. Grow 16-18 hours @ 37 C and 220 rpm</p><br>
-     <p class="w3-justify" style="font-size:25px;padding-left:180px;">Day 3:</p>
-    <p class="w3-justify" style="font-size:20px;padding-left:240px">
+     <p class="w3-justify" style="font-size:20px;padding-left:240px">Day 3:
        Dilution cultures in LB+Chloramphenicol <br>
-       Measure OD<sub>600</sub><br>
+       <p class="w3-justify" style="font-size:20px;padding-left:304px;">Measure OD<sub>600</sub><br></p>
-       Further dilute<br>
+       <p class="w3-justify" style="font-size:20px;padding-left:304px;">Further dilute<br></p>
-       Incubate<br>
+       <p class="w3-justify" style="font-size:20px;padding-left:304px;">Incubate<br></p>
-       Abs600 and fluorescence measurement<br>
+       <p class="w3-justify" style="font-size:20px;padding-left:304px;">Abs600 and fluorescence measurement<br></p>
-       Record measurements<br>
+       <p class="w3-justify" style="font-size:20px;padding-left:304px;">Record measurements<br></p>
-       Fill out corresponding Excel sheet<br>
+       <p class="w3-justify" style="font-size:20px;padding-left:304px;">Fill out corresponding Excel sheet<br></p>
@@ Line 99: / Line 110: @@
      </p><br>
-     <p class="w3-center" style="font-size:30px;">Protocol：Colony Forming Units per 0.1 OD<sub>600</sub>  E.coli cultures</p>
+     <p class="w3-justify" style="font-size:25px;padding-left:150px">✔ Protocol：Colony Forming Units per 0.1 OD<sub>600</sub>  E.coli cultures</p>
+  <p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
+      <p class="w3-justify" style="font-size:25px;padding-left:150px">✔ To see the results, <a href="https://docs.google.com/spreadsheets/d/1GZ5xY4s5LGc7funlSDGDNl71Atd4TWjkDY37gka57aA/edit#gid=211336931"><src="https://docs.google.com/spreadsheets/d/1GZ5xY4s5LGc7funlSDGDNl71Atd4TWjkDY37gka57aA/edit#gid=211336931">click here</a>.</p>