Cyclohexane (Talk | contribs) |
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<p class="indent">This year we have designed more than 20 parts, half are basic parts, the other are composite ones. The parts are all designed by ourselves and most of them have been tested to insure their reliability. To see their specific function, you can click <span><a class="click_here" href="https://2018.igem.org/Team:XMU-China/Design">here</a></span> to learn about our project, or browse the specific page on <span><a class="click_here" href="http://parts.igem.org/Main_Page">parts.igem.org</a></span> by clicking on the part numbers.</p> | <p class="indent">This year we have designed more than 20 parts, half are basic parts, the other are composite ones. The parts are all designed by ourselves and most of them have been tested to insure their reliability. To see their specific function, you can click <span><a class="click_here" href="https://2018.igem.org/Team:XMU-China/Design">here</a></span> to learn about our project, or browse the specific page on <span><a class="click_here" href="http://parts.igem.org/Main_Page">parts.igem.org</a></span> by clicking on the part numbers.</p> | ||
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<p class="indent">This year we have designed eleven basic parts mostly from the Kai ABC systems. <a href="http://parts.igem.org/Part:BBa_K2623003">BBa_K2623003</a> is our favorite basic part, which is the core part of the Kai circuit, acting as a hexamer. This part can be both auto phosphorylated and dephosphorylated, and it can achieve about 24 hours of phosphorylation-dephosphorylation oscillation under the regulation of KaiA and KaiB. | <p class="indent">This year we have designed eleven basic parts mostly from the Kai ABC systems. <a href="http://parts.igem.org/Part:BBa_K2623003">BBa_K2623003</a> is our favorite basic part, which is the core part of the Kai circuit, acting as a hexamer. This part can be both auto phosphorylated and dephosphorylated, and it can achieve about 24 hours of phosphorylation-dephosphorylation oscillation under the regulation of KaiA and KaiB. | ||
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<p class="indent">We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is <a href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a>, which links <a href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> and <a href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a>, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatche.</p> | <p class="indent">We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is <a href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a>, which links <a href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> and <a href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a>, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatche.</p> | ||
<table class="table"> | <table class="table"> |
Revision as of 13:44, 11 October 2018
This year we have designed more than 20 parts, half are basic parts, the other are composite ones. The parts are all designed by ourselves and most of them have been tested to insure their reliability. To see their specific function, you can click here to learn about our project, or browse the specific page on parts.igem.org by clicking on the part numbers.
This year we have designed eleven basic parts mostly from the Kai ABC systems. BBa_K2623003 is our favorite basic part, which is the core part of the Kai circuit, acting as a hexamer. This part can be both auto phosphorylated and dephosphorylated, and it can achieve about 24 hours of phosphorylation-dephosphorylation oscillation under the regulation of KaiA and KaiB.
Favorite | Register Name | Code used in Lab | Description | Types | Lenth | Designer |
---|---|---|---|---|---|---|
R | BBa_K2623003 | KaiC | KaiC coding region | Coding | 1560 bp | Xinyi Ren |
BBa_K2623001 | KaiA | KaiA coding region | Coding | 852 bp | Yi Lin | |
BBa_K2623002 | KaiB | KaiB coding region | Coding | 309 bp | Yuhan Qin | |
BBa_K2623004 | SasA | SasA coding region | Coding | 1167 bp | Yi Lin | |
BBa_K2623005 | CikA | CikA coding region | Coding | 2265 bp | Yuhan Qin | |
BBa_K2623006 | paA | paA coding region | Coding | 753 bp | Xinyi Ren | |
BBa_K2623007 | SAHS | SAHS coding region | Coding | 510 bp | Yingming Zhu | |
BBa_K2623008 | CAHS | CAHS coding region | Coding | 714 bp | Weizhao Li | |
BBa_K2623009 | pKaiBC | pKaiBC promoter | egulatory | 89 bp | Gezhi Xiao | |
BBa_K2623040 | OmpA leader | OmpA leader sequence | Coding | 72 bp | Niangui Cai | |
BBa_K2623027 | NG7 | siRNA with C/Dbox RNA structure to bind with L7Ae (BBa_K2623026) | Composite | 1447 bp | Yunyun Hu |
We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is BBa_K2623028, which links BBa_K2623022 and BBa_K2623025, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatche.
Favorite | Register Name | Code used in Lab | Description | Types | Lenth | Designer |
---|---|---|---|---|---|---|
R | BBa_K2623028 | NG8-1 | A composite part linked by BBa_K2623022 and BBa_K2623025 | Composite | 3410 bp | Niangui Cai |
BBa_K2623011 | Kai 2 | Phosphorylation pathsway builder of Kai | Composite | 4444 bp | Gezhi Xiao | |
BBa_K2623013 | Kai 1 3 | Kai's phosphorylation core oscllator builder and SfYFP reportor for pKaiBC | Composite | 3978 bp | Hongling Liu | |
BBa_K2623014 | CAHS 1 | Cytosolic-abundant heat soluble protein "CAHS " (promoter, RBS, RFP, lacI and double terminator) | Composite | 2891 bp | Hongling Liu | |
BBa_K2623015 | SAHS 1 | Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, RFP and double terminator) | Composite | 1425 bp | Jun Dai | |
BBa_K2623016 | SAHS 2 | Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, amilCP and double terminator) | Composite | 1431 bp | Yuanjun Shang | |
BBa_K2623021 | NG1 | BBa_K2623022 with GFP at its N-termini. | Reporter | 1657 bp | Niangui Cai | |
BBa_K2623022 | NG2 | Bacterial outer membrane protein A (OmpA) fused with SpyTag at its C-termini | Composite | 910 bp | Qiupeng Wang | |
BBa_K2623023 | NG3 | Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini. | Reporter | 1645 bp | Junhong Chen | |
BBa_K2623024 | NG4 | A non-GFP form of the BBa_K2623023 | Composite | 898 bp | Jiyang Zheng | |
BBa_K2623025 | NG5 | Archaeal ribosomal protein protein L7Ae fused with SpyCatcher and mRFP1. | Reporter | 2613 bp | Ruofan Yang | |
BBa_K2623026 | NG6 | Archaeal ribosomal protein protein L7Ae fused with SpyCatcher. | Composite | 2317 bp | Yunyun Hu | |
BBa_K2623029 | NG8-2 | A composite part linked by BBa_K2623022 and BBa_K2623026. | Composite | 2698 bp | Ye Qiang | |
BBa_K2623030 | NG9-1 | A composite part linked by BBa_K2623024 and BBa_K2623025. | Composite | 3410 bp | Yang Liang | |
BBa_K2623031 | NG9-2 | A composite part linked by BBa_K2623024 and BBa_K2623026 | Composite | 2698 bp | Junhong Chen | |
BBa_K2623032 | NG10 | A composite part linked by BBa_K2623024, BBa_K2623026 and BBa_K2623027 | Composite | 4153 bp | Niangui Cai |