Difference between revisions of "Team:NTHU Formosa/InterLab"

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     <p class="w3-justify" style="font-size:25px;padding-left:150px">✔ Cell Measurement protocol</p>
 
     <p class="w3-justify" style="font-size:25px;padding-left:150px">✔ Cell Measurement protocol</p>
 
<p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
 
<p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
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     <p class="w3-justify" style="font-size:20px;padding-left:240px">Day 1:Transform E.Coli DH5α with plasmids (all in pSB1C3)</p>
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     <p class="w3-justify" style="font-size:20px;padding-left:240px">Day 1: Transform E.Coli DH5α with plasmids (all in pSB1C3)</p>
 
      
 
      
  
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     <p class="w3-justify" style="font-size:20px;padding-left:240px">Day 2:pick 2 colonies per plate (5 mL LB medium + Chloramphenicol.  </p>
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     <p class="w3-justify" style="font-size:20px;padding-left:240px">Day 2: Pick 2 colonies per plate (5 mL LB medium + Chloramphenicol.  </p>
 
      
 
      
 
     <p class="w3-justify" style="font-size:20px;padding-left:296px;">Grow 16-18 hours @ 37 C and 220 rpm</p>
 
     <p class="w3-justify" style="font-size:20px;padding-left:296px;">Grow 16-18 hours @ 37 C and 220 rpm</p>

Revision as of 04:04, 13 October 2018

IGEM 2018 Interlab Study

Overall To-Do List



✔ Calibration 1：OD600 Reference point - LUDOX Protocol


1.Record settings for standard measurements

2.Record measurements

3.Fill out corresponding Excel sheet


✔ Calibration 2：​Particle Standard Curve - Microsphere Protocol


1.Prepare Microsphere Stock Solution

2.Serial dilution

3.Measure Abs600 of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet


✔ Calibration 3：Fluorescence standard curve - Fluorescein Protocol


1.Resuspend and dilute fluorescein

2.Serial dilutions

3.Measure fluorescence of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet


✔ Cell Measurement protocol


Day 1: Transform E.Coli DH5α with plasmids (all in pSB1C3)

Day 2: Pick 2 colonies per plate (5 mL LB medium + Chloramphenicol.

Grow 16-18 hours @ 37 C and 220 rpm

Day 3: Dilution cultures in LB+Chloramphenicol

Measure OD600

Further dilute

Incubate

Abs600 and fluorescence measurement

Record measurements

Fill out corresponding Excel sheet


✔ Protocol：Colony Forming Units per 0.1 OD600 E.coli cultures


✔ To see the results, click here.