This year, we constructed plenty of parts that can be categorized into 3 groups:

1. The plasmids for FRET Model

This group ranges from part BBa_K275001 to BBa_K275007. The parts are the ones that we primarily constructed and utilized in the FRET system. BBa_K275001 is a pET32a with its MCS replaced with sequences designed by us. The replacement sequence contains a specifically designed MCS that can be inserted with a FRET protein and a DKK1-binding protein and express the two proteins as a fusion protein under the control of lac operon of pET32a. Hence, part BBa_K275003 to part BBa_K275007 are all cloned using BBa_K275001.