Difference between revisions of "Team:NYMU-Taipei/Part Collection"

 
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<p>This year, we constructed plenty of parts that can be categorized into 3 groups:</p>
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<h2>1. The plasmids for FRET Model</h2>
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<p>This group ranges from <a href="http://parts.igem.org/Part:BBa_K2751000">part BBa_K2751000</a> to <a href = "http://parts.igem.org/Part:BBa_K2751007">BBa_K2751007</a>. The parts are the ones that we primarily constructed and utilized in the FRET system. <a href="http://parts.igem.org/Part:BBa_K2751000">BBa_K2751000</a> is a pET32a with its MCS replaced with sequences designed by us. The replacement sequence contains a specifically designed MCS that can be inserted with a FRET protein and a DKK1-binding protein and express the two proteins as a fusion protein under the control of lac operon of pET32a. Hence, <a href="http://parts.igem.org/Part:BBa_K2751003">part BBa_K2751003</a> to <a href="http://parts.igem.org/Part:BBa_K2751007">part BBa_K2751007</a> are all cloned using <a href="http://parts.igem.org/Part:BBa_K2751000">BBa_K2751000.</a></p>
  
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<h2>2. The Submitted Parts</h2>
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<p>Ranging from <a href="http://parts.igem.org/Part:BBa_K2751008">BBa_K2751008</a> to <a href="http://parts.igem.org/Part:BBa_K2751015">BBa+K2751015</a> are the parts that we sent for the medal criteria. Since we have establish a system that is suitable for our project but completely different from RFC 10 regulations this year, we can only submit a portion of the parts we have created or there would be too many point mutations to make. While all of them except mEGFP, which is assigned as the improved part, are functional and eligible for silver medal's validated part criteria, <a href="http://parts.igem.org/Part:BBa_K2751013">BBa_K2751013</a> stood out as our favorite composite part and <a href="http://parts.igem.org/Part:BBa_K2751015">BBa_K2751015</a> is our favorite basic part.</p>
  
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<h2>3. The plasmids for Cell Model</h2>
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<p>The Cell Model has 4 major contructs: from <a href="http://parts.igem.org/Part:BBa_K2751024">BBa_K2751024</a> to <a href="http://parts.igem.org/Part:BBa_K2751027">BBa_K2751027</a>. They are the plasmids that are transformed into HEK293 and DP for fluorescence exhibition.</p>
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<groupparts>iGEM18 NYMU-Taipei</groupparts>
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<h1> Part Collection </h1>
 
<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
 
 
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While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.
 
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<h3>Note</h3>
 
<p>This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
 
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<h3>Best Part Collection Special Prize</h3>
 
<p>To be eligible for this award, these parts must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a collection of parts you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part numbers to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.</p>
 
 
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Latest revision as of 17:22, 14 October 2018




This year, we constructed plenty of parts that can be categorized into 3 groups:

1. The plasmids for FRET Model

This group ranges from part BBa_K2751000 to BBa_K2751007. The parts are the ones that we primarily constructed and utilized in the FRET system. BBa_K2751000 is a pET32a with its MCS replaced with sequences designed by us. The replacement sequence contains a specifically designed MCS that can be inserted with a FRET protein and a DKK1-binding protein and express the two proteins as a fusion protein under the control of lac operon of pET32a. Hence, part BBa_K2751003 to part BBa_K2751007 are all cloned using BBa_K2751000.

2. The Submitted Parts

Ranging from BBa_K2751008 to BBa+K2751015 are the parts that we sent for the medal criteria. Since we have establish a system that is suitable for our project but completely different from RFC 10 regulations this year, we can only submit a portion of the parts we have created or there would be too many point mutations to make. While all of them except mEGFP, which is assigned as the improved part, are functional and eligible for silver medal's validated part criteria, BBa_K2751013 stood out as our favorite composite part and BBa_K2751015 is our favorite basic part.

3. The plasmids for Cell Model

The Cell Model has 4 major contructs: from BBa_K2751024 to BBa_K2751027. They are the plasmids that are transformed into HEK293 and DP for fluorescence exhibition.


<groupparts>iGEM18 NYMU-Taipei</groupparts>