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Revision as of 20:21, 15 October 2018
IGEM 2018 Interlab Study
Overall To-Do List
✔ Calibration 1：OD600 Reference point - LUDOX Protocol
1.Record settings for standard measurements
2.Record measurements
3.Fill out corresponding Excel sheet
✔ Calibration 2：Particle Standard Curve - Microsphere Protocol
1.Prepare Microsphere Stock Solution
2.Serial dilution
3.Measure Abs600 of all samples in instrument
4.Record measurements
5.Fill out corresponding Excel sheet
✔ Calibration 3：Fluorescence standard curve - Fluorescein Protocol
1.Resuspend and dilute fluorescein
2.Serial dilutions
3.Measure fluorescence of all samples in instrument
4.Record measurements
5.Fill out corresponding Excel sheet
✔ Cell Measurement protocol
Day 1: Transform E.Coli DH5α with plasmids (all in pSB1C3)
Day 2: Pick 2 colonies per plate (5 mL LB medium + Chloramphenicol.
Grow 16-18 hours @ 37 C and 220 rpm
Day 3:
Dilution cultures in LB+Chloramphenicol
Measure OD600
Further dilute
Incubate
Abs600 and fluorescence measurement
Record measurements
Fill out corresponding Excel sheet
✔ Protocol：Colony Forming Units per 0.1 OD600 E.coli cultures
✔ To see the results,