Difference between revisions of "Team:LZU-CHINA/Notebook"

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{{LZU-CHINA}}
 
 
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<h1>Notebook</h1>
 
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
 
  
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</div>
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<script type="text/javascript" src="https://2018.igem.org/wiki/index.php?title=Template:LZU-CHINA/Javascript/jquery1&action=raw&ctype=text/javascript"></script>
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<div class="clear"></div>
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<link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:LZU-CHINA/CSS&action=raw&ctype=text/css">
  
  
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<link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:LZU-CHINA/CSS/bookstrap&action=raw&ctype=text/css">
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<h3>What should this page have?</h3>
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<link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:LZU-CHINA/CSS/demo&action=raw&ctype=text/css">
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<ul>
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<link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:LZU-CHINA/CSS/jquery_DonutWidget&action=raw&ctype=text/css">
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<li>Chronological notes of what your team is doing.</li>
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<link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:LZU-CHINA/CSS/jquerysctipttop&action=raw&ctype=text/css">
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<li> Brief descriptions of daily important events.</li>
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<link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:LZU-CHINA/CSS/linearicons&action=raw&ctype=text/css">
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<li>Pictures of your progress. </li>
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<link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:LZU-CHINA/CSS/magnific_popup&action=raw&ctype=text/css">
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<li>Mention who participated in what task.</li>
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<link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:LZU-CHINA/CSS/nice_select&action=raw&ctype=text/css">
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</ul>
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<link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:LZU-CHINA/CSS/owl_carousel&action=raw&ctype=text/css">
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<li><a href="https://2018.igem.org/Team:LZU-CHINA">Home</a></li>
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<a class="dropdown-item" href="https://2018.igem.org/Team:LZU-CHINA/Team">Team</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:LZU-CHINA/Attributions">Attribution</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:LZU-CHINA/Collaborations">collaborations</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:LZU-CHINA/Description">Description</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:LZU-CHINA/Design">Design</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:LZU-CHINA/Parts">part</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:LZU-CHINA/Experiments">experiments</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:LZU-CHINA/Safety">safety</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:LZU-CHINA/Results">result</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:LZU-CHINA/Notebook">notebook</a>
  
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</div>
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<div class="highlight decoration_A_full">
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</li>
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<h3>Inspiration</h3>
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<li><a href="https://2018.igem.org/Team:LZU-CHINA/Model">dry lab</a></li>
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<p>You can see what others teams have done to organize their notes:</p>
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<a class="dropdown-toggle" href="https://2018.igem.org/Team:LZU-CHINA/Human_Practices" id="4" data-toggle="dropdown">HP</a>
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<div class="dropdown-menu">
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<a class="dropdown-item" href="https://2018.igem.org/Team:LZU-CHINA/Human_Practices">intergrated hp</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:LZU-CHINA/Public_Engagement">public engagement</a>
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<div class="generic-banner-content">
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<h2 class="text-white">Notebook</h2>
  
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<ul>  
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</div>
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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</div>
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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</div>
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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</div>
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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</section>
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</ul>
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</div>
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</div>
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<!-- About Generic Start -->
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<div class="main-wrapper">
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<div class="location" >
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<p  style="text-align: left;"><a href="#p1" style="font-size: 14px;color: #FFFFFF;margin-bottom: 16px;">1.Overview</a></p>
 +
<p  style="text-align: left;"><a href="#p2" style="font-size: 14px;color: #FFFFFF;margin-bottom: 16px;">2.2017</a></p>
 +
<p  style="text-align: left;"><a href="#p3" style="font-size: 14px;color: #FFFFFF;margin-bottom: 20px;">3.2018</a></p>
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<p class="scroll_top" style="text-align: center;font-size: 16px;">RETURN TOP</p>
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</div>
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<!-- Start Generic Area -->
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<section class="about-generic-area pt-100" id="p1">
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<div class="container" style="background: rgb(255,255,255)">
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<div style="height: 50px"></div>
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<div class="col-md-12">
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<div class="myimg"><img src="https://static.igem.org/mediawiki/2018/b/b8/T--LZU-CHINA--notebook1.jpg"  class="img-fluid mr-20 mb-20"style="width: 80%" ></div>
 +
<div class="myimg"><img src="https://static.igem.org/mediawiki/2018/e/ec/T--LZU-CHINA--notebook222.jpg"  class="img-fluid mr-20 mb-20"style="width: 80%" ></div>
 +
</div>
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<div style="height: 20px" id="p2"></div>
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<h3 class="about-title mb-30" >2017</h3>
 +
<div class="progress-table-wrap">
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<div class="progress-table">
 +
<div class="table-head">
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<div class="notebook1">Month</div>
 +
<div class="notebook2">What we have done</div>
 +
</div>
 +
<div class="table-row">
 +
<div class="notebook1">June</div>
 +
<div class="notebook2">Design IGEM engineering scheme.</div>
 +
</div>
 +
<div class="table-row">
 +
<div class="notebook1">July and August</div>
 +
<div class="notebook2">Explore the culture conditions of different gastric cancer cell lines.</div>
 +
</div>
 +
<div class="table-row">
 +
<div class="notebook1">September, October</div>
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<div class="notebook2">Design IGEM engineering scheme.</div>
 +
</div>
 +
<div class="table-row">
 +
<div class="notebook1">November </div>
 +
<div class="notebook2">Lentiviral vector construction and partial parts synthesis.</div>
 +
</div>
 +
</div>
 +
</div>
 +
<div style="height: 20px" id="p3"></div>
 +
<h3 class="about-title mb-30" >2018</h3>
 +
<div class="progress-table-wrap">
 +
<div class="progress-table">
 +
<div class="table-head">
 +
<div class="notebook1">Month</div>
 +
<div class="notebook2">What we have done</div>
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</div>
 +
<div class="table-row">
 +
<div class="notebook1">February</div>
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<div class="notebook2">Obtain stable cell line of miR attacker.</div>
 +
</div>
 +
<div class="table-row">
 +
<div class="notebook1">March</div>
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<div class="notebook2">Detect the effect of micro RNA on gastric cancer cell line through scratch test and CCK-8 experiment.</div>
 +
</div>
 +
<div class="table-row">
 +
<div class="notebook1">April</div>
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<div class="notebook2">Detect the effect of microRNA on cell cycle of gastric cancer cell lines by flow cytometry.</div>
 +
</div>
 +
<div class="table-row">
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<div class="notebook1">May  </div>
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<div class="notebook2">Get stable cell line of Exosome booster.</div>
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</div>
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<div class="table-row">
 +
<div class="notebook1">June</div>
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<div class="notebook2">Exosomes were isolated and identified by WB and electron microscopy.</div>
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</div>
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<div class="table-row">
 +
<div class="notebook1">July</div>
 +
<div class="notebook2">NTA analysis validates Exosome booster’s function.</div>
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</div>
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<div class="table-row">
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<div class="notebook1">August</div>
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<div class="notebook2">Confocal microscopy was used to verify exosomes uptake.</div>
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</div>
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<div class="table-row">
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<div class="notebook1">September</div>
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<div class="notebook2">Build IGEM parts and submit to IGEM headquarters.</div>
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</div>
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<div class="table-row">
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<div class="notebook1">October</div>
 +
<div class="notebook2">Detect the transcription efficiency of induced promoters under different inductors. And, Giant Jamboree~</div>
 +
</div>
 +
</div>
 +
</div>
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</div>
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</section>
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<!-- End Generic Start -->
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<!-- Start Generic Area -->
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<section class="about-generic-area pb-100 pt-100" id="safety2">
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</div>
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</section>
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<!-- End Generic Start -->
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<!-- start footer Area -->
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<footer class="footer-area section-gap">
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<div class="container">
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<div class="row">
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<div class="col-lg-6 col-md-6 col-sm-6">
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<div class="single-footer-widget">
 +
<h6>About Us</h6>
 +
<img src="https://static.igem.org/mediawiki/2018/e/ee/T--LZU-CHINA--xiaohui.png" style="height: 120px">
 +
<img src="https://static.igem.org/mediawiki/2018/d/df/T--LZU-CHINA--yiyuan.png" style="height: 120px">
 +
<img src="https://static.igem.org/mediawiki/2018/4/41/T--LZU-CHINA--shengming.png" style="height: 120px">
 +
<p>
 +
We wish to extend our deepest appreciation to the Lanzhou University, The First Clinical Medical College and School of Life Science for the great support and help in this project
 +
</p>
 +
</div>
 +
</div>
 +
 
 +
<div class="col-lg-6 col-md-6 col-sm-6 social-widget">
 +
<div class="single-footer-widget">
 +
<h6>Contact Us</h6>
 +
<p>E-mail：752786236@qq.com 1098385458@qq.com</p>
 +
<p>Address：No. 222, Tianshui South Road, chengguan district, Lanzhou City, Gansu Province,730000, P. R. China.</p>
 +
<p style="font-size:20px;font-family: 'Segoe Script'">We are the walker on the long journey towards science</p>
 +
 +
<p class="footer-text">Copyright &copy;2018 iGEM LZU-CHINA Team All rights reserved.</p>
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Latest revision as of 05:43, 16 October 2018

Notebook

1.Overview

2.2017

3.2018

RETURN TOP

2017

Month
What we have done
June
Design IGEM engineering scheme.
July and August
Explore the culture conditions of different gastric cancer cell lines.
September, October
Design IGEM engineering scheme.
November
Lentiviral vector construction and partial parts synthesis.

2018

Month
What we have done
February
Obtain stable cell line of miR attacker.
March
Detect the effect of micro RNA on gastric cancer cell line through scratch test and CCK-8 experiment.
April
Detect the effect of microRNA on cell cycle of gastric cancer cell lines by flow cytometry.
May
Get stable cell line of Exosome booster.
June
Exosomes were isolated and identified by WB and electron microscopy.
July
NTA analysis validates Exosome booster’s function.
August
Confocal microscopy was used to verify exosomes uptake.
September
Build IGEM parts and submit to IGEM headquarters.
October
Detect the transcription efficiency of induced promoters under different inductors. And, Giant Jamboree~
About Us

We wish to extend our deepest appreciation to the Lanzhou University, The First Clinical Medical College and School of Life Science for the great support and help in this project