Mar

- Our team was formally founded.
- All student members learned the fundamental knowledge of molecular cloning and iGEM.
- All student members looked through the previous excellent projects and learned the basic notion of synthetic biology and iGEM.

Apr

- We were divided into four groups and started to brainstorm separately (and we reported each’s progress every weekend).
- Our team was formally accepted by iGEM.

May

- Our formal project was confirmed.
- The pre-experiment plan was confirmed.
- The division of labor was confirmed.

Jun Wk1-3

-All student members learned laboratory fundamental qualities and received molecular cloning operation training.

Jun Wk 4

- 2018 DNA Distribution Kit Plates were received.
- We transformed the biobricks which we needed.
- BBa_J04450 constructed to get the backbone.

Jul Wk 3

– The genetic circuit of the TDP began to be built.

Jul Wk 4

– Interlab experiments.

– Verified the activity of EpCAM.

Aug Wk 1

– The genetic circuit of the Kai began to be built.

Aug Wk 2

– BBa_K2623015 constructed.

- BBa_K2623009constructed.

– BBa_K2623016constructed.

- The genetic circuit of SAHS was completed and protein validation began.

Aug Wk 3

- BBa_K2623001 constructed.

- BBa_K2623022 and BBa_K2623024 constructed.

- BBa_I13401 constructed to get the GFP reporter.

Aug Wk 4

- BBa_K2623021 and BBa_K2623023 constructed.

- BBa_K2623026 constructed.

- Aptamer and ssDNA successfully combined.

- BBa_K2623002 constructed.

- BBa_K2623003 constructed.

– Verified that SYL3C aptamer could combine with 10nt complementary chain successfully.

Sep Wk 1

- BBa_K2623014 constructed.

Sep Wk 2

- GFP on BBa_K2623021 and BBa_K2623023 successfully expressed.

- BBa_K2623004 constructed.

- BBa_K2623005 constructed.

- BBa_K2623006 constructed.

- First time successful purification of high-purity SAHS protein.

- Coating experiment on microfluidic chips succeeded.
- Started bacterial freeze-drying experiment.

Sep Wk 3

- BBa_E1010 constructed to get the mRFP reporter.

- BBa_K2623011 constructed.

- BBa_K2623007 constructed.

- Competition experiment succeeded.
- Started protein freeze-drying experiment.

– Verified the feasibility of “competition method” successfully.

Sep Wk 4

- BBa_K2623025 constructed.

- BBa_K2623008 constructed.

- Started try genetic recombination experiment.

Oct Wk 1

- BBa_K2623027 constructed.

- BBa_K2623013 constructed.

- NG2+5, NG2+6, NG4+5, NG4+6 constructed.

– Verified that As Cas12a could be activated.

Oct Wk 2

- BBa_K2623028, BBa_K2623029, BBa_K2623030, BBa_K2623031 successfully constructed and the fluorescence intensity of OMVs isolated from E.coli containing these four parts were detected by flow cytometer

– Verified the successful combination between SYL3C aptamer and EpCAM.