Difference between revisions of "Team:BioMarvel/Notebook/Lab Journal"

 
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<h1>July</h1>
 
<h1>July</h1>
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<a name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><div class="mNoteBookJH"><h4>7/9/2018 </h4></div></a><DIV style='display:none'>
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<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/9/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
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<div class="mNoteBookJ">
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<p>Measurement of Abs <sub>600</sub> and OD <sub>600</sub> for LUDOX CL-X and water</p>
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<p>Measurement of Abs 600 and OD 600 for LUDOX CL-Xand water</p>
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<p>Preparation of a dilution series of silica microspheres and measurement of the Abs <sub>600</sub> in a plate reader</p>
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<p>Prepared a dilution series of silica microspheres and measure the Abs 600 in a plate reader</p>
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<p>Preparation of a dilution series of fluorescein in four replicates and measurement of the fluorescence</p>
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<p>Prepared a dilution series of fluorescein in four replicates and measure the fluorescence</p>
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<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A>
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</div><A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV>
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</DIV>
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<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/10/2018 – 7/22/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
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<p>Transformation of <i>E. coli</i> DH5α with InterLab study plasmids</p>
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<div class="mNoteBookJH"><h4>7/9/2018 </h4></div>
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<p>Picking colonies from the transformed plates and inoculation in 5 mL LB medium + Chloramphenicol and overnight culture</p>
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<div class="mNoteBookJ">
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<p>Sampling and assay</p>
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<p>Measurement of Abs 600 and OD 600 for LUDOX CL-Xand water</p>
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<p style="color: red;">Triplication of the same procedures to confirm the reproducibility</p>
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<p>Prepared a dilution series of silica microspheres and measure the Abs 600 in a plate reader</p>
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<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV>
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<p>Prepared a dilution series of fluorescein in four replicates and measure the fluorescence</p>
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<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/23/2018 (Composite parts cloning started) ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
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</div>
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<p>DNA fragments were constructed from Integrated DNA Technologies (IDT)</p>
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<div class="mNoteBookJH"><h4>7/10/2018 – 7/22/2018</h4></div>
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<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV>
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<div class="mNoteBookJ">
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<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/24/2018 – 7/31/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
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<p>Transform <i>E. coli</i> DH5α with InterLab study plasmids</p>
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<p>A-tailing process at the end of our gene fragment for TA cloning</p>
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<p>Pick colonies from each of the transformation plates and inoculate in 5-10 mL LB medium +</p>
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<p>Ligation of the fragment with TA vector</p>
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<p>Chloramphenicol. Grow the cells overnight. Cell growth, sampling, and assay</p>
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<p>Transformation of DH5 α <i>E. coli</i> with the ligated vector (Blue-white screen)</p>
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<p style="color: red;">Repeat experiments were conducted to confirm the reproducibility of the data</p>
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<p>Picking colonies from the plates and overnight culture with 0.1 mM of ampicillin </p>
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</div>
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<p>Isolation of the amplified plasmid using DNA mini prep kit</p>
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<div class="mNoteBookJH"><h4>7/23/2018 (Composite parts cloning started)</h4></div>
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<p>Purification of the DNA fragment gel electrophoresis and ligation with pSB1C3.</p>
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<div class="mNoteBookJ">
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<p>Verification of appropriate insertion of the construct in the vector</p>
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<p>DNA fragments werereceivedfrom Integrated DNA Technologies (IDT)</p>
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</div>
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<div class="mNoteBookJH"><h4>7/24/2018 – 7/31/2018</h4></div>
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<div class="mNoteBookJ">
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<p>Conducted A-tailing process at the end of our gene fragment for TA cloning</p>
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<p>A-tailed gene fragment was ligated with the TA vector</p>
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−
<p>Transformed<i>E. coli</i> DH5α with ligated TA vector (Blue-white screen)</p>
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<p>Picked colonies from transformation plates. Cells were grown in LB broth with 0.1mM of ampicillin</p>
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<p>Vector isolation was conducted through DNA mini prep kit</p>
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<p>DNA fragment parts were purified using gel electrophoresis and ligated with pSB1C3 vector.</p>
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<p>Checked appropriate insertion of the construct in the vector </p>
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<br>
 
<br>
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<p style="color: red;">Repeat experiments were conducted for debugging</p>
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<p style="color: red;">Duplication of the same procedures for debugging</p>
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</div>
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<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV>
 
</section>
 
</section>
 
<section id="mSection">
 
<section id="mSection">
 
<h1>August</h1>
 
<h1>August</h1>
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<div class="mNoteBookJH"><h4>8/1/2018 – 8/11/2018</h4></div>
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<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>8/1/2018 – 8/11/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
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<div class="mNoteBookJ">
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<p>Sequence analysis (BIOFACT™ sequencing analysis service)</p>
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<p>Plasmid sequence was confirmed by BIOFACT™ sequencing analysis service</p>
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<p>Amplification and purification of GBP-ProG fusion Protein</p>
 
<p>Amplification and purification of GBP-ProG fusion Protein</p>
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<p>Purified proteins were confirmed by SDS-PAGE</p>
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<p>Size confirmation of the purified proteins with SDS-PAGE</p>
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<p>Protein quantified using the Bradford protein assay</p>
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<p>Quantification of the proteins with the Bradford protein assay</p>
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</div>
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<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV>
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<div class="mNoteBookJH"><h4>8/12/2018 – 8/19/2018</h4></div>
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<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>8/12/2018 – 8/19/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
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<div class="mNoteBookJ">
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<p>Fabrication of interdigitated array (IDA) gold electrode</p>
 
<p>Fabrication of interdigitated array (IDA) gold electrode</p>
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<p>Fabricatedelectrochemical immunochip</p>
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<p>Fabrication of electrochemical immunochip</p>
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<p>Cyclic voltammograms were measured for electrochemical detection</p>
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<p>Measurement of cyclic voltammograms for detecting NTproBNP</p>
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<p style="color: red;">Repeat experiments were conducted to confirm the reproducibility of the data</p>
+
<p style="color: red;">Triplication of the same procedures for reproducibility</p>
−
</div>
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<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV>
 
</section>
 
</section>
 
<section id="mSection">
 
<section id="mSection">
 
<h1>September</h1>
 
<h1>September</h1>
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<div class="mNoteBookJH"><h4>September</h4></div>
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<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>9/10/2018 (Basic parts cloning started) ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
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<div class="mNoteBookJ">
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<p>PCR primers construction from BIOFACT™</p>
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<p>PCR primers werereceivedfrom BIOFACT™</p>
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<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV>
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</div>
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<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>9/11/2018 – 9/22/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
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<div class="mNoteBookJH"><h4>9/11/2018 – 9/22/2018</h4></div>
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<p>Insertion of the DNA fragment into pSB1C3 vector in the same manner as mentioned in composite part cloning</p>
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<div class="mNoteBookJ">
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<p>Cloning for two basic parts (GBP and ProG) separately</p>
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<p>A series of experiments was the same as a composite parts cloning experiment.</p>
+
<p>Sequence analysis (BIOFACT™ sequencing analysis service)</p>
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<p>Conducted cloning experiments for two basic parts (GBP, ProG)</p>
+
<p style="color: red;">Duplication of the same procedures for debugging</p>
−
<p>Plasmid sequence was confirmed by BIOFACT™ sequencing analysis service</p>
+
<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV>
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<br>
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<p style="color: red;">Repeat experiments were conducted for debugging</p>
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</div>
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</section>
 
</section>
 
</div>
 
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Latest revision as of 06:41, 16 October 2018

≡
　

July

7/9/2018 ▾

7/10/2018 – 7/22/2018 ▾

7/23/2018 (Composite parts cloning started) ▾

7/24/2018 – 7/31/2018 ▾

August

8/1/2018 – 8/11/2018 ▾

8/12/2018 – 8/19/2018 ▾

September

9/10/2018 (Basic parts cloning started) ▾

9/11/2018 – 9/22/2018 ▾

#iGEM #2018 #BioMarvel #Heart #Biochip #Diagnosis

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IGEM2018BioMarvel@gmail.com

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