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<section id="mSection"> | <section id="mSection"> | ||
<h1>July</h1> | <h1>July</h1> | ||
− | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/9/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'> | |
− | + | <p>Measurement of Abs <sub>600</sub> and OD <sub>600</sub> for LUDOX CL-X and water</p> | |
− | + | <p>Preparation of a dilution series of silica microspheres and measurement of the Abs <sub>600</sub> in a plate reader</p> | |
− | + | <p>Preparation of a dilution series of fluorescein in four replicates and measurement of the fluorescence</p> | |
− | + | ||
− | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/9/2018</h4></a><DIV class="mNoteBookJ" style='display:none'> | + | |
− | <p>Measurement of Abs 600 and OD 600 for LUDOX CL- | + | |
− | <p> | + | |
− | <p> | + | |
<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A> | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A> | ||
</DIV> | </DIV> | ||
− | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/10/2018 – 7/22/2018</h4></a><DIV class="mNoteBookJ" style='display:none'> | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/10/2018 – 7/22/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'> |
− | <p> | + | <p>Transformation of <i>E. coli</i> DH5α with InterLab study plasmids</p> |
− | <p> | + | <p>Picking colonies from the transformed plates and inoculation in 5 mL LB medium + Chloramphenicol and overnight culture</p> |
− | <p> | + | <p>Sampling and assay</p> |
− | <p style="color: red;"> | + | <p style="color: red;">Triplication of the same procedures to confirm the reproducibility</p> |
<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | ||
− | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/23/2018 (Composite parts cloning started)</h4></a><DIV style='display:none'> | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/23/2018 (Composite parts cloning started) ▾</h4></a><DIV class="mNoteBookJ" style='display:none'> |
− | <p>DNA fragments | + | <p>DNA fragments were constructed from Integrated DNA Technologies (IDT)</p> |
<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | ||
− | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/24/2018 – 7/31/2018</h4></a><DIV class="mNoteBookJ" style='display:none'> | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/24/2018 – 7/31/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'> |
− | <p> | + | <p>A-tailing process at the end of our gene fragment for TA cloning</p> |
− | <p> | + | <p>Ligation of the fragment with TA vector</p> |
− | <p> | + | <p>Transformation of DH5 α <i>E. coli</i> with the ligated vector (Blue-white screen)</p> |
− | <p> | + | <p>Picking colonies from the plates and overnight culture with 0.1 mM of ampicillin </p> |
− | <p> | + | <p>Isolation of the amplified plasmid using DNA mini prep kit</p> |
− | <p>DNA fragment | + | <p>Purification of the DNA fragment gel electrophoresis and ligation with pSB1C3.</p> |
− | <p> | + | <p>Verification of appropriate insertion of the construct in the vector</p> |
<br> | <br> | ||
− | <p style="color: red;"> | + | <p style="color: red;">Duplication of the same procedures for debugging</p> |
<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | ||
</section> | </section> | ||
<section id="mSection"> | <section id="mSection"> | ||
<h1>August</h1> | <h1>August</h1> | ||
− | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>8/1/2018 – 8/11/2018</h4></a><DIV class="mNoteBookJ" style='display:none'> | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>8/1/2018 – 8/11/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'> |
− | <p> | + | <p>Sequence analysis (BIOFACT™ sequencing analysis service)</p> |
<p>Amplification and purification of GBP-ProG fusion Protein</p> | <p>Amplification and purification of GBP-ProG fusion Protein</p> | ||
− | <p> | + | <p>Size confirmation of the purified proteins with SDS-PAGE</p> |
− | <p> | + | <p>Quantification of the proteins with the Bradford protein assay</p> |
<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | ||
− | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>8/12/2018 – 8/19/2018</h4></a><DIV class="mNoteBookJ" style='display:none'> | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>8/12/2018 – 8/19/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'> |
− | <p> | + | <p>Fabrication of interdigitated array (IDA) gold electrode</p> |
− | <p> | + | <p>Fabrication of electrochemical immunochip</p> |
− | <p> | + | <p>Measurement of cyclic voltammograms for detecting NTproBNP</p> |
− | <p> | + | <p style="color: red;">Triplication of the same procedures for reproducibility</p> |
<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | ||
</section> | </section> | ||
<section id="mSection"> | <section id="mSection"> | ||
<h1>September</h1> | <h1>September</h1> | ||
− | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>9/10/2018 (Basic parts cloning started)</h4></a><DIV class="mNoteBookJ" style='display:none'> | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>9/10/2018 (Basic parts cloning started) ▾</h4></a><DIV class="mNoteBookJ" style='display:none'> |
− | <p>PCR primers | + | <p>PCR primers construction from BIOFACT™</p> |
<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | ||
− | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>9/11/2018 – 9/22/2018</h4></a><DIV class="mNoteBookJ" style='display:none'> | + | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>9/11/2018 – 9/22/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'> |
− | <p> | + | <p>Insertion of the DNA fragment into pSB1C3 vector in the same manner as mentioned in composite part cloning</p> |
− | <p> | + | <p>Cloning for two basic parts (GBP and ProG) separately</p> |
− | <p> | + | <p>Sequence analysis (BIOFACT™ sequencing analysis service)</p> |
− | + | <p style="color: red;">Duplication of the same procedures for debugging</p> | |
− | <p style="color: red;"> | + | |
<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | ||
</section> | </section> |
Latest revision as of 06:41, 16 October 2018