Difference between revisions of "Team:BioMarvel/Notebook/Lab Journal"

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 			<h1>July</h1>
 			<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/9/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
-				<p>Measurement of Abs 600 and OD 600 for LUDOX CL-Xand water</p>
+				<p>Measurement of Abs <sub>600</sub> and OD <sub>600</sub> for LUDOX CL-X and water</p>
-				<p>Prepared a dilution series of silica microspheres and measure the Abs 600 in a plate reader</p>
+				<p>Preparation of a dilution series of silica microspheres and measurement of the Abs <sub>600</sub> in a plate reader</p>
-				<p>Prepared a dilution series of fluorescein in four replicates and measure the fluorescence</p>
+				<p>Preparation of a dilution series of fluorescein in four replicates and measurement of the fluorescence</p>
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 			</DIV>
 			<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/10/2018 – 7/22/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
-				<p>Transform <i>E. coli</i> DH5α with InterLab study plasmids</p>
+				<p>Transformation of <i>E. coli</i> DH5α with InterLab study plasmids</p>
-				<p>Pick colonies from each of the transformation plates and inoculate in 5-10 mL LB medium +</p>
+				<p>Picking colonies from the transformed plates and inoculation in 5 mL LB medium + Chloramphenicol and overnight culture</p>
-				<p>Chloramphenicol. Grow the cells overnight. Cell growth, sampling, and assay</p>
+				<p>Sampling and assay</p>
-				<p style="color: red;">Repeat experiments were conducted to confirm the reproducibility of the data</p>
+				<p style="color: red;">Triplication of the same procedures to confirm the reproducibility</p>
 			<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV>
 			<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/23/2018 (Composite parts cloning started) ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
-				<p>DNA fragments werereceivedfrom Integrated DNA Technologies (IDT)</p>
+				<p>DNA fragments were constructed from Integrated DNA Technologies (IDT)</p>
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 			<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/24/2018 – 7/31/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
-				<p>Conducted A-tailing process at the end of our gene fragment for TA cloning</p>
+				<p>A-tailing process at the end of our gene fragment for TA cloning</p>
-				<p>A-tailed gene fragment was ligated with the TA vector</p>
+				<p>Ligation of the fragment with TA vector</p>
-				<p>Transformed<i>E. coli</i> DH5α with ligated TA vector (Blue-white screen)</p>
+				<p>Transformation of DH5 α <i>E. coli</i> with the ligated vector (Blue-white screen)</p>
-				<p>Picked colonies from transformation plates. Cells were grown in LB broth with 0.1mM of ampicillin</p>
+				<p>Picking colonies from the plates and overnight culture with 0.1 mM of ampicillin </p>
-				<p>Vector isolation was conducted through DNA mini prep kit</p>
+				<p>Isolation of the amplified plasmid using DNA mini prep kit</p>
-				<p>DNA fragment parts were purified using gel electrophoresis and ligated with pSB1C3 vector.</p>
+				<p>Purification of the DNA fragment gel electrophoresis and ligation with pSB1C3.</p>
-				<p>Checked appropriate insertion of the construct in the vector </p>
+				<p>Verification of appropriate insertion of the construct in the vector</p>
 				<br>
-				<p style="color: red;">Repeat experiments were conducted for debugging</p>
+				<p style="color: red;">Duplication of the same procedures for debugging</p>
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 		</section>
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 			<h1>August</h1>
 			<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>8/1/2018 – 8/11/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
-				<p>Plasmid sequence was confirmed by BIOFACT™ sequencing analysis service</p>
+				<p>Sequence analysis (BIOFACT™ sequencing analysis service)</p>
 				<p>Amplification and purification of GBP-ProG fusion Protein</p>
-				<p>Purified proteins were confirmed by SDS-PAGE</p>
+				<p>Size confirmation of the purified proteins with SDS-PAGE</p>
-				<p>Protein quantified using the Bradford protein assay</p>
+				<p>Quantification of the proteins with the Bradford protein assay</p>
 			<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV>
 			<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>8/12/2018 – 8/19/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
-				<p>Plasmid sequence was confirmed by BIOFACT™ sequencing analysis service</p>
+				<p>Fabrication of interdigitated array (IDA) gold electrode</p>
-				<p>Amplification and purification of GBP-ProG fusion Protein</p>
+				<p>Fabrication of electrochemical immunochip</p>
-				<p>Purified proteins were confirmed by SDS-PAGE</p>
+				<p>Measurement of cyclic voltammograms for detecting NTproBNP</p>
-				<p>Protein quantified using the Bradford protein assay</p>
+				<p style="color: red;">Triplication of the same procedures for reproducibility</p>
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 			<h1>September</h1>
 			<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>9/10/2018 (Basic parts cloning started) ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
-				<p>PCR primers werereceivedfrom BIOFACT™</p>
+				<p>PCR primers construction from BIOFACT™</p>
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 			<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>9/11/2018 – 9/22/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
-				<p>A series of experiments was the same as a composite parts cloning experiment.</p>
+				<p>Insertion of the DNA fragment into pSB1C3 vector in the same manner as mentioned in composite part cloning</p>
-				<p>Conducted cloning experiments for two basic parts (GBP, ProG)</p>
+				<p>Cloning for two basic parts (GBP and ProG) separately</p>
-				<p>Plasmid sequence was confirmed by BIOFACT™ sequencing analysis service</p>
+				<p>Sequence analysis (BIOFACT™ sequencing analysis service)</p>
-				<br>
+				<p style="color: red;">Duplication of the same procedures for debugging</p>
-				<p style="color: red;">Repeat experiments were conducted for debugging</p>
 			<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV>
 		</section>