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<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/9/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'> | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/9/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'> | ||
<p>Measurement of Abs <sub>600</sub> and OD <sub>600</sub> for LUDOX CL-X and water</p> | <p>Measurement of Abs <sub>600</sub> and OD <sub>600</sub> for LUDOX CL-X and water</p> | ||
− | <p>Preparation a dilution series of silica microspheres and | + | <p>Preparation of a dilution series of silica microspheres and measurement of the Abs <sub>600</sub> in a plate reader</p> |
− | <p>Preparation a dilution series of fluorescein in four replicates and | + | <p>Preparation of a dilution series of fluorescein in four replicates and measurement of the fluorescence</p> |
<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A> | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A> | ||
</DIV> | </DIV> | ||
<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/10/2018 – 7/22/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'> | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/10/2018 – 7/22/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'> | ||
<p>Transformation of <i>E. coli</i> DH5α with InterLab study plasmids</p> | <p>Transformation of <i>E. coli</i> DH5α with InterLab study plasmids</p> | ||
− | <p>Picking colonies from | + | <p>Picking colonies from the transformed plates and inoculation in 5 mL LB medium + Chloramphenicol and overnight culture</p> |
<p>Sampling and assay</p> | <p>Sampling and assay</p> | ||
− | <p style="color: red;"> | + | <p style="color: red;">Triplication of the same procedures to confirm the reproducibility</p> |
<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | ||
<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/23/2018 (Composite parts cloning started) ▾</h4></a><DIV class="mNoteBookJ" style='display:none'> | <a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/23/2018 (Composite parts cloning started) ▾</h4></a><DIV class="mNoteBookJ" style='display:none'> | ||
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<p>Isolation of the amplified plasmid using DNA mini prep kit</p> | <p>Isolation of the amplified plasmid using DNA mini prep kit</p> | ||
<p>Purification of the DNA fragment gel electrophoresis and ligation with pSB1C3.</p> | <p>Purification of the DNA fragment gel electrophoresis and ligation with pSB1C3.</p> | ||
− | <p> | + | <p>Verification of appropriate insertion of the construct in the vector</p> |
<br> | <br> | ||
− | <p style="color: red;"> | + | <p style="color: red;">Duplication of the same procedures for debugging</p> |
<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | ||
</section> | </section> | ||
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<p>Insertion of the DNA fragment into pSB1C3 vector in the same manner as mentioned in composite part cloning</p> | <p>Insertion of the DNA fragment into pSB1C3 vector in the same manner as mentioned in composite part cloning</p> | ||
<p>Cloning for two basic parts (GBP and ProG) separately</p> | <p>Cloning for two basic parts (GBP and ProG) separately</p> | ||
− | <p>Sequence analysis ( | + | <p>Sequence analysis (BIOFACT™ sequencing analysis service)</p> |
− | <p style="color: red;"> | + | <p style="color: red;">Duplication of the same procedures for debugging</p> |
<A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | <A onclick=this.parentNode.style.display='none'; href="#aa">[Close]</A></DIV> | ||
</section> | </section> |
Latest revision as of 06:41, 16 October 2018