Difference between revisions of "Team:BioMarvel/Notebook/Lab Journal"

 
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<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/9/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
 
<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/9/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
 
<p>Measurement of Abs <sub>600</sub> and OD <sub>600</sub> for LUDOX CL-X and water</p>
 
<p>Measurement of Abs <sub>600</sub> and OD <sub>600</sub> for LUDOX CL-X and water</p>
<p>Preparation a dilution series of silica microspheres and measure the Abs <sub>600</sub> in a plate reader</p>
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<p>Preparation of a dilution series of silica microspheres and measurement of the Abs <sub>600</sub> in a plate reader</p>
<p>Preparation a dilution series of fluorescein in four replicates and measure the fluorescence</p>
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<p>Preparation of a dilution series of fluorescein in four replicates and measurement of the fluorescence</p>
 
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</DIV>
 
</DIV>
 
<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/10/2018 – 7/22/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
 
<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/10/2018 – 7/22/2018 ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
 
<p>Transformation of <i>E. coli</i> DH5α with InterLab study plasmids</p>
 
<p>Transformation of <i>E. coli</i> DH5α with InterLab study plasmids</p>
<p>Picking colonies from each of the transformation plates and inoculation in 5 mL LB medium + Chloramphenicol and overnight culture</p>
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<p>Picking colonies from the transformed plates and inoculation in 5 mL LB medium + Chloramphenicol and overnight culture</p>
 
<p>Sampling and assay</p>
 
<p>Sampling and assay</p>
<p style="color: red;">Triplicating the same procedures to confirm the reproducibility</p>
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<p style="color: red;">Triplication of the same procedures to confirm the reproducibility</p>
 
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<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/23/2018 (Composite parts cloning started) ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
 
<a class="mNoteBookJH" name="aa" href='javascript:void(0)' onclick=this.nextSibling.style.display=(this.nextSibling.style.display=='none')?'block':'none';><h4>7/23/2018 (Composite parts cloning started) ▾</h4></a><DIV class="mNoteBookJ" style='display:none'>
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<p>Isolation of the amplified plasmid using DNA mini prep kit</p>
 
<p>Isolation of the amplified plasmid using DNA mini prep kit</p>
 
<p>Purification of the DNA fragment gel electrophoresis and ligation with pSB1C3.</p>
 
<p>Purification of the DNA fragment gel electrophoresis and ligation with pSB1C3.</p>
<p>Verifying appropriate insertion of the construct in the vector</p>
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<p>Verification of appropriate insertion of the construct in the vector</p>
 
<br>
 
<br>
<p style="color: red;">Duplicating the same procedures for debugging</p>
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<p style="color: red;">Duplication of the same procedures for debugging</p>
 
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</section>
 
</section>
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<p>Insertion of the DNA fragment into pSB1C3 vector in the same manner as mentioned in composite part cloning</p>
 
<p>Insertion of the DNA fragment into pSB1C3 vector in the same manner as mentioned in composite part cloning</p>
 
<p>Cloning for two basic parts (GBP and ProG) separately</p>
 
<p>Cloning for two basic parts (GBP and ProG) separately</p>
<p>Sequence analysis (BIOFACTTM sequencing analysis service)</p>
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<p>Sequence analysis (BIOFACT™ sequencing analysis service)</p>
<p style="color: red;">Duplicating the same procedures for debugging</p>
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<p style="color: red;">Duplication of the same procedures for debugging</p>
 
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</section>
 
</section>

Latest revision as of 06:41, 16 October 2018

 

July

7/9/2018 ▾

7/10/2018 – 7/22/2018 ▾

7/23/2018 (Composite parts cloning started) ▾

7/24/2018 – 7/31/2018 ▾

August

8/1/2018 – 8/11/2018 ▾

8/12/2018 – 8/19/2018 ▾

September

9/10/2018 (Basic parts cloning started) ▾

9/11/2018 – 9/22/2018 ▾

#iGEM #2018 #BioMarvel #Heart #Biochip #Diagnosis

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IGEM2018BioMarvel@gmail.com

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