Measurement of Abs ₆₀₀ and OD ₆₀₀ for LUDOX CL-X and water

Preparation of a dilution series of silica microspheres and measurement of the Abs ₆₀₀ in a plate reader

Preparation of a dilution series of fluorescein in four replicates and measurement of the fluorescence

Transformation of E. coli DH5α with InterLab study plasmids

Picking colonies from the transformed plates and inoculation in 5 mL LB medium + Chloramphenicol and overnight culture

Sampling and assay

Triplication of the same procedures to confirm the reproducibility

DNA fragments were constructed from Integrated DNA Technologies (IDT)

A-tailing process at the end of our gene fragment for TA cloning

Ligation of the fragment with TA vector

Transformation of DH5 α E. coli with the ligated vector (Blue-white screen)

Picking colonies from the plates and overnight culture with 0.1 mM of ampicillin

Isolation of the amplified plasmid using DNA mini prep kit

Purification of the DNA fragment gel electrophoresis and ligation with pSB1C3.

Verification of appropriate insertion of the construct in the vector

Duplication of the same procedures for debugging

Sequence analysis (BIOFACT™ sequencing analysis service)

Amplification and purification of GBP-ProG fusion Protein

Size confirmation of the purified proteins with SDS-PAGE

Quantification of the proteins with the Bradford protein assay

Fabrication of interdigitated array (IDA) gold electrode

Fabrication of electrochemical immunochip

Measurement of cyclic voltammograms for detecting NTproBNP

Triplication of the same procedures for reproducibility

PCR primers construction from BIOFACT™

Insertion of the DNA fragment into pSB1C3 vector in the same manner as mentioned in composite part cloning

Cloning for two basic parts (GBP and ProG) separately

Sequence analysis (BIOFACT™ sequencing analysis service)

Duplication of the same procedures for debugging