Difference between revisions of "Team:CCU Taiwan/Basic Part"
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}); | }); | ||
}); | }); | ||
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// The function actually applying the offset | // The function actually applying the offset | ||
function offsetAnchor() { | function offsetAnchor() { | ||
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'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div> | 'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div> | ||
| − | <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a | + | <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a> |
<ul class="sub" id="sub_home" style="cursor:default;"> | <ul class="sub" id="sub_home" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a> | ||
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id=" | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a> |
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id=" | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a> |
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a | + | <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a> |
<ul class="sub" id="sub_project" style="cursor:default;"> | <ul class="sub" id="sub_project" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a> | ||
| Line 99: | Line 100: | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a> | ||
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab "><li class="list" id="project5">InterLab</li></a> | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab"><li class="list" id="project5">InterLab</li></a> |
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a | + | <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a> |
<ul class="sub" id="sub_parts" style="cursor:default;"> | <ul class="sub" id="sub_parts" style="cursor:default;"> | ||
| + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Parts"><li class="list" id="parts1">Overview</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a> | ||
| − | |||
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a | + | <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a> |
<ul class="sub" id="sub_modeling" style="cursor:default;"> | <ul class="sub" id="sub_modeling" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a> | ||
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding</li></a> | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding Model</li></a> |
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer</li></a> | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer Model</li></a> |
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a | + | <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a> |
<ul class="sub" id="sub_drylab" style="cursor:default;"> | <ul class="sub" id="sub_drylab" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a> | ||
| Line 123: | Line 124: | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a | + | <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a> |
<ul class="sub" id="sub_human_practice" style="cursor:default;"> | <ul class="sub" id="sub_human_practice" style="cursor:default;"> | ||
| − | <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1"> | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Human Practice</li></a> |
<a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | ||
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</header> | </header> | ||
| + | |||
<div class="backgroundParts"> | <div class="backgroundParts"> | ||
Revision as of 09:22, 16 October 2018
BASIC PART
BBa_K2809001
Kozak sequence (Pichia pastoris)
The Kozak sequence initiates the translation in Pichia pastoris. This Sequence is copied directly from pCore1 in reference. The start codon is not included.
BBa_K2809002
Prepro-alpha-factor (codon modified for P. pastoris)
Prepro-alpha-factor is the secretion signal peptide from the yeast α-mating factor. This signal peptide directs expression of secretion of the recombinant protein, therefore, it allows the exportation of it. Once the recombinant protein is in the endoplasmic reticulum (ER) lumen, this secretion signal will be cleaved.
BBa_K2809010
Mature Lac1 (laccase)
This is the codon-optimized version of laccase derived from Pinus taeda (Loblolly pine). Laccase is involved in monolignol dehydrogenation, using oxygen to oxidize their metal centers to enable catalytical phenol oxidation. It is able to produce dimers and oligomers of coniferyl alcohol, which is involved in the early stage of lignification.
The signal peptide has been removed, so the sequence does NOT contain a start codon. Furthermore, it includes HA-tag for Western Blot protein detection and 6*His-tag for protein purification before the stop codon.
BBa_K2809020
Mature Px18 (peroxidase)
Those are the codon-optimized versions of peroxidase derived from Picea abies (Norway spruce). Peroxidases (Px16 and Px18) are involved in monolignol dehydrogenation, using hydrogen peroxide as a substrate. They are able to form polymers from oligomers or dimers, which contribute to lignin polymerization.
The signal peptide has been removed, so the sequence does NOT contain a start codon. Moreover, it includes V5-tag for Western Blot protein detection and 6*His-tag for protein purification before the stop codon.
BBa_K2809030
Mature Px16 (peroxidase)
Those are the codon-optimized versions of peroxidase derived from Picea abies (Norway spruce). Peroxidases (Px16 and Px18) are involved in monolignol dehydrogenation, using hydrogen peroxide as a substrate. They are able to form polymers from oligomers or dimers, which contribute to lignin polymerization.
The signal peptide has been removed, so the sequence does NOT contain a start codon. Furthermore, it includes FLAG-tag for Western Blot protein detection and 6*His-tag for protein purification before the stop codon.