Difference between revisions of "Team:CCU Taiwan/Our Plan"
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| − | <p class="description">   There is a difference between samples of coniferyl alcohol without reacting with enzymes and smaples with Laccase and Peroxidase. The absorption peak shifted to the left, comparing with simulations (P. J. Salazar-Valencia et al. 2006), we can prove that the bonds we estimate did appear.</p> | + | <p class="description" id="halftext">   There is a difference between samples of coniferyl alcohol without reacting with enzymes and smaples with Laccase and Peroxidase. The absorption peak shifted to the left, comparing with simulations (P. J. Salazar-Valencia et al. 2006), we can prove that the bonds we estimate did appear.</p> |
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Revision as of 12:33, 16 October 2018
ANALYSIS
We analyzed the lignin-like polymer we produced to confirm the work is successful. In the first stage of analysis, we started by analyzing the content and structure of our lignin-like polymer by UV-visible spectroscopy. For the second stage, we used NMR spectroscopy and Mass Spectroscopy to analyze information about the molecular structure and molecular weight of our polymer. In the last stage, Thermogravimetric analysis is used to analyze the melting or decomposition point of our product. After the above experiments are completed, we have a deeper understanding of the lignin-like polymer we have made. These experimental data also help us in the application and improvement of the products.
Reaction experiment
There is a difference between samples of coniferyl alcohol without reacting with enzymes and smaples with Laccase and Peroxidase. The absorption peak shifted to the left, comparing with simulations (P. J. Salazar-Valencia et al. 2006), we can prove that the bonds we estimate did appear.
Figure1: UV-Visible spectrum of products
Figure2: NMR Spectrum of coniferyl alcohol
Figure3: NMR Spectrum of commercial dealkaline lignin
Figure4: NMR Spectrum of Laccase-involved reaction product
Figure5: NMR Spectrum of Laccase & Peroxidase-involved reaction product