Difference between revisions of "Team:CCU Taiwan/Our Plan"

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<div id="halftext"><p class="description">&emsp;&emsp; Chemical shift</p></div>
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                    <p>Figure3: Chemical shift of coniferyl alcohol</p>
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                   <img src="https://static.igem.org/mediawiki/2018/4/4d/T--CCU_Taiwan--CCUconuferylNMR.png" width="100%">
 
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                     <p>Figure3: NMR Spectrum of coniferyl alcohol</p>
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                     <p>Figure4: NMR Spectrum of coniferyl alcohol</p>
 
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                     <p>Figure4: NMR Spectrum of commercial dealkaline lignin</p>
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                     <p>Figure5: NMR Spectrum of commercial dealkaline lignin</p>
 
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                     <p>Figure5: NMR Spectrum of Laccase-involved reaction product</p>
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                     <p>Figure6: NMR Spectrum of Laccase-involved reaction product</p>
 
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                   <img src="https://static.igem.org/mediawiki/2018/8/8f/T--CCU_Taiwan--CCUlaccaseandperoxidaseNMR.jpg" width="100%">
 
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                     <p>Figure6: NMR Spectrum of Laccase & Peroxidase-involved reaction product</p>
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                     <p>Figure7: NMR Spectrum of Laccase & Peroxidase-involved reaction product</p>
 
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Revision as of 13:20, 16 October 2018

ANALYSIS



  We analyzed the lignin-like polymer we produced to confirm the work is successful. In the first stage of analysis, we started by analyzing the content and structure of our lignin-like polymer by UV-visible spectroscopy. For the second stage, we used NMR spectroscopy and Mass Spectroscopy to analyze information about the molecular structure and molecular weight of our polymer. In the last stage, Thermogravimetric analysis is used to analyze the melting or decomposition point of our product. After the above experiments are completed, we have a deeper understanding of the lignin-like polymer we have made. These experimental data also help us in the application and improvement of the products.



Reaction experiment


   There is a difference between samples of coniferyl alcohol without reacting with enzymes and smaples with Laccase and Peroxidase. From the result, we can prove that the reaction has occurred. The absorption peak shifted to the left, comparing with simulation from study (P. J. Salazar-Valencia et al. 2005), the wavelength of β-5 linkage absorption peak is shorter. Our results are similar to the results of the study, which means our product has β-5 linkage.


Figure1: UV-Visible spectrum of products

Figure2: Simulation of the electronic or ultraviolet spectra for the 3 Coniferyl Alcohol units, with β-O-4 and β-5 linkages. (P. J. Salazar-Valencia et al. 2005)

   Chemical shift

Figure3: Chemical shift of coniferyl alcohol

Figure4: NMR Spectrum of coniferyl alcohol

Figure5: NMR Spectrum of commercial dealkaline lignin

Figure6: NMR Spectrum of Laccase-involved reaction product

Figure7: NMR Spectrum of Laccase & Peroxidase-involved reaction product



Reference

P. J. Salazar-Valencia, S. T. P´erez-Merchancano, and L. E. Bol´ıvar-Marin´ez. (2005). Optical Properties in Biopolymers: Lignin Fragments. Brazilian Journal of Physics, vol. 36, no. 3B.