Difference between revisions of "Team:NTHU Formosa/InterLab"

Line 8: Line 8:
 
   <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/initial&action=raw&ctype=text/css">
 
   <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/initial&action=raw&ctype=text/css">
 
   <link rel="stylesheet" type="text/css" href="//fonts.googleapis.com/css?family=Josefin+Sans" />
 
   <link rel="stylesheet" type="text/css" href="//fonts.googleapis.com/css?family=Josefin+Sans" />
 +
<link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/wordcss&action=raw&ctype=text/css">
  
 
   <style>
 
   <style>
Line 27: Line 28:
 
       color: hotpink;
 
       color: hotpink;
 
       cursor: pointer;
 
       cursor: pointer;
−
    }
 
−
    .w3-center{
 
−
        font-weight: 900;
 
−
        font-size: 350%;
 
−
        font-family:  "Josefin Sans","JosefinSans-Bold",NotoSansCJKtc-Regular,'Noto Sans',"微軟雅黑","Microsoft YaHei",Helvetica,sans-serif;
 
 
     }
 
     }
  
−
    .w3-wide{
 
−
        font-weight: 900;
 
−
        font-size: 350%;
 
−
        font-family:  "Josefin Sans","JosefinSans-Bold",NotoSansCJKtc-Regular,'Noto Sans',"微軟雅黑","Microsoft YaHei",Helvetica,sans-serif;
 
−
    }
 
−
 
−
    .w3-justify{
 
−
        font-size: 22px;
 
−
        font-family: "Josefin Sans","JosefinSans-Regular",Open Sans,"Helvetica Neue",Helvetica,Arial,sans-serif;
 
−
    }
 
  
 
     /* selected link */
 
     /* selected link */

Revision as of 15:36, 16 October 2018

IGEM 2018 Interlab Study

Overall To-Do List



✔ Calibration 1：OD600 Reference point - LUDOX Protocol


1.Record settings for standard measurements

2.Record measurements

3.Fill out corresponding Excel sheet


✔ Calibration 2：​Particle Standard Curve - Microsphere Protocol


1.Prepare Microsphere Stock Solution

2.Serial dilution

3.Measure Abs600 of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet


✔ Calibration 3：Fluorescence standard curve - Fluorescein Protocol


1.Resuspend and dilute fluorescein

2.Serial dilutions

3.Measure fluorescence of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet


✔ Cell Measurement protocol


Day 1: Transform E.Coli DH5α with plasmids (all in pSB1C3)

Day 2: Pick 2 colonies per plate (5 mL LB medium + Chloramphenicol.

Grow 16-18 hours @ 37 C and 220 rpm

Day 3: Dilution cultures in LB+Chloramphenicol

Measure OD600

Further dilute

Incubate

Abs600 and fluorescence measurement

Record measurements

Fill out corresponding Excel sheet


✔ Protocol：Colony Forming Units per 0.1 OD600 E.coli cultures


✔ To see the results, click here.