Difference between revisions of "Team:NTHU Formosa/InterLab"

Revision as of 16:20, 16 October 2018

IGEM 2018 Interlab Study

Overall To-Do List

✔ Calibration 1：OD₆₀₀ Reference point - LUDOX Protocol

1.Record settings for standard measurements

2.Record measurements

3.Fill out corresponding Excel sheet

✔ Calibration 2：Particle Standard Curve - Microsphere Protocol

1.Prepare Microsphere Stock Solution

2.Serial dilution

3.Measure Abs600 of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet

✔ Calibration 3：Fluorescence standard curve - Fluorescein Protocol

1.Resuspend and dilute fluorescein

2.Serial dilutions

3.Measure fluorescence of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet

✔ Cell Measurement protocol

Day 1: Transform E.Coli DH5α with plasmids (all in pSB1C3)

Day 2: Pick 2 colonies per plate (5 mL LB medium + Chloramphenicol.

Grow 16-18 hours @ 37 C and 220 rpm

Day 3: Dilution cultures in LB+Chloramphenicol

Measure OD₆₀₀

Further dilute

Incubate

Abs600 and fluorescence measurement

Record measurements

Fill out corresponding Excel sheet

✔ Protocol：Colony Forming Units per 0.1 OD₆₀₀ E.coli cultures

✔ To see the results, click here.

@@ Line 63: / Line 63: @@
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">4.Record measurements</p>
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">5.Fill out corresponding Excel sheet</p><br>
-     <p class="w3-justify" style="font-size:25px;padding-left:150px;">✔ Calibration 3：Fluorescence standard curve - Fluorescein Protocol </p>
+     <p class="w3-justify" style="font-size:25px;padding-left:150px;"><b>✔ Calibration 3：Fluorescence standard curve - Fluorescein Protocol </b></p>
      <p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
@@ Line 72: / Line 72: @@
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">4.Record measurements</p>
      <p class="w3-justify" style="font-size:20px;padding-left:240px;">5.Fill out corresponding Excel sheet</p><br>
-     <p class="w3-justify" style="font-size:25px;padding-left:150px">✔ Cell Measurement protocol</p>
+     <p class="w3-justify" style="font-size:25px;padding-left:150px"><b>✔ Cell Measurement protocol</b></p>
 <p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
      <p class="w3-justify" style="font-size:20px;padding-left:240px">Day 1: Transform E.Coli DH5α with plasmids (all in pSB1C3)</p>
@@ Line 95: / Line 95: @@
      </p><br>
-     <p class="w3-justify" style="font-size:25px;padding-left:150px">✔ Protocol：Colony Forming Units per 0.1 OD<sub>600</sub>  E.coli cultures</p>
+     <p class="w3-justify" style="font-size:25px;padding-left:150px"><b>✔ Protocol：Colony Forming Units per 0.1 OD<sub>600</sub>  E.coli cultures</b></p>
    <p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
-       <p class="w3-justify" style="font-size:25px;padding-left:150px">✔ To see the results, <a href="https://docs.google.com/spreadsheets/d/1GZ5xY4s5LGc7funlSDGDNl71Atd4TWjkDY37gka57aA/edit#gid=211336931"><src="https://docs.google.com/spreadsheets/d/1GZ5xY4s5LGc7funlSDGDNl71Atd4TWjkDY37gka57aA/edit#gid=211336931">click here</a>.</p>
+       <p class="w3-justify" style="font-size:25px;padding-left:150px"><b>✔ To see the results, </b><a href="https://docs.google.com/spreadsheets/d/1GZ5xY4s5LGc7funlSDGDNl71Atd4TWjkDY37gka57aA/edit#gid=211336931"><src="https://docs.google.com/spreadsheets/d/1GZ5xY4s5LGc7funlSDGDNl71Atd4TWjkDY37gka57aA/edit#gid=211336931">click here</a>.</p>