Difference between revisions of "Team:XMU-China/Composite Part"

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{{XMU-China}}
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    <title>Team:XMU-China/Composite Parts - 2018.igem.org</title>
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    <link rel="stylesheet" href="css/font.css">
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    <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/cover?action=raw&ctype=text/css">
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    <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/nav?action=raw&ctype=text/css">
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    <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/parts?action=raw&ctype=text/css">
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</head>
  
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<body>
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<div class="column full_size judges-will-not-evaluate">
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    <header></header>
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<h3>★  ALERT! </h3>
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    <!-- <div id="container">
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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        <header>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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            <div class="wrapper cf">
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</div>
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                <nav id="main-nav">
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                    <ul class="first-nav">
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                        <li class="Project">
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<div class="clear"></div>
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                            <a href="#" target="_blank">Project</a>
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                            <ul>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Description">Description</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Design">Design</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Results">Results</a></li>
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<div class="column full_size">
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Parts">Parts</a></li>
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<h1>Composite Parts</h1>
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                            </ul>
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                        </li>
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                    </ul>
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<p>
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                    <ul class="second-nav">
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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                        <li class="Hardware">
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</p>
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                            <a href="#">Hardware</a>
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                            <ul>
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Hardware">Overview</a></li>
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</div>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Microfluidic_Chips">Microfluidic chips</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Fluorescenc_Detection">Fluorescence Detection</a></li>
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<div class="column full_size">
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Straberry_Pi">Straberry Pi</a></li>
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<div class="highlight decoration_background">
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Applied_Design">Applied Design</a></li>
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<h3>Note</h3>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Software">APP</a></li>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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                            </ul>
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</div>
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                        </li>
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</div>
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                        <li class="Model">
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                            <a href="#">Model</a>
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                            <ul>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Model">Overview</a></li>
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<div class="column full_size">
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                            </ul>
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<h3>Best Composite Part Special Prize</h3>
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                        </li>
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                        <li class="Human_Practice">
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<p>To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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                            <a href="#">Human Practice</a>
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                            <ul>
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<br><br>
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                                <li><a href="https://2018.igem.org/Te
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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                                am:XMU-China/Human_Practices">Overview</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/HP/Silver">Silver</a></li>
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</div>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/HP/Gold_Integrated">Gold</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Public_Engagement">Engagement</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Entrepreneurship">Entrepreneurship</a></li>
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                            </ul>
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                        </li>
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                        <li class="Other_Works">
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                            <a href="#">Other Works</a>
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                            <ul>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/InterLab">InterLab</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Improve">Improve</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Safety">Safety</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Measurement">Measurement</a></li>
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                            </ul>
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                        </li>
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                        <li class="Notebook">
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                            <a href="#">Notebook</a>
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                            <ul>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Notebook">Notebook</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Experiments">Experiments</a></li>
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                            </ul>
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                        </li>
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                        <li class="Team">
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                            <a href="#">Team</a>
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                            <ul>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Team">Members</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Attributions">Attributions</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Judging">Judging</a></li>
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                            </ul>
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                        </li>
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                    </ul>
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                </nav>
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                <a class="toggle"><span></span></a>
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    <div class="header">
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            <img src="https://static.igem.org/mediawiki/2018/b/b5/T--XMU-China--singlelogo.png">
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            <div class="clear"></div>
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            <!-- <div class="nav">
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                <div id="Team">
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                    <div class="nav-word">Team</div>
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                    <ul>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Team">Members</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Attributions">Attributions</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Judging">Judging</a></li>
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                    </ul>
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                </div>
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                <div id="Notebook">
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                    <div>
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                        <div class="nav-word">Notebook</a></div>
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                    </div>
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                    <ul>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Notebook">Notebook</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Experiments">Experiments</a></li>
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                    </ul>
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                </div>
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                <div id="Other_Works">
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                    <div class="nav-word">Other Works</div>
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                    <ul>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/InterLab">InterLab</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Improve">Improve</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Safety">Safety</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Measurement">Measurement</a></li>
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                    </ul>
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                </div>
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                <div id="Human_Practice">
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                    <div class="nav-word">Human Practice</div>
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                    <ul>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Human_Practices">Overview</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/HP/Silver">Silver</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/HP/Gold_Integrated">Gold</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Public_Engagement">Engagement</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Entrepreneurship">Entrepreneurship</a></li>
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                    </ul>
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                </div>
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                <div id="Model">
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                    <div class="nav-word">Model</div>
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                    <ul>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Model">Overview</a></li>
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                    </ul>
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                </div>
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                <div id="Hardwork">
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                    <div class="nav-word">Hardwork</div>
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                    <ul>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Hardware">Overview</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Microfluidic_Chips">Microfluidic chips</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Fluorescenc_Detection">Fluorescence Detection</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Straberry_Pi">Straberry Pi</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Applied_Design">Applied Design</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Software">APP</a></li>
 +
                    </ul>
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                </div>
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                <div id="Project">
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                    <div class="nav-word">Project</div>
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                    <ul>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Description">Description</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Design">Design</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Results">Results</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Parts">Parts</a></li>
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                    </ul>
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                </div>
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                <div id="jiannan">
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                    <a href="https://2018.igem.org/Team:XMU-China">
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                        <img src="https://static.igem.org/mediawiki/2018/6/6f/T--XMU-China--jiannanlogo.png">
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                    </a>
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        <div class="description_banner">
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            <div class="word">Composite Parts</div>
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        </div>
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        <!-- <nav class="Quick-navigation">
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                <img  src="https://static.igem.org/mediawiki/2018/e/e6/T--XMU-China--right50.png">
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                <a href="#Cell_Measurement" class="Quick-navigation-item">
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                    <img id="turn_img" src="https://static.igem.org/mediawiki/2018/5/50/T--XMU-China--right51.png">
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                    <a href="#Cell_Measurement"id="Quick_A">Cell Measurement</a></a>
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                <a href="#Colony_Forming_Units" class="Quick-navigation-item" >
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                    <a href="#Colony_Forming_Units" id="Quick_B">Colony Forming Units</a></a>
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                <a href="#Experience_and_Discussion" class="Quick-navigation-item">
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                    <img id="turn_img" src="https://static.igem.org/mediawiki/2018/d/d0/T--XMU-China--right53.png">
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                    <a href="#Experience_and_Discussion" id="Quick_C">Experience and Discussion</a></a>
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                <a href="#Provenance" class="Quick-navigation-item">
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                    <img id="turn_img" src="https://static.igem.org/mediawiki/2018/1/11/T--XMU-China--right54.png">
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                    <a href="#Provenance"id="Quick_D">Provenance</a></a>
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                <a href="#Reference" class="Quick-navigation-item">
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                    <img id="turn_img" src="https://static.igem.org/mediawiki/2018/b/ba/T--XMU-China--right55.png">
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                    <a href="#Reference"id="Quick_F">Reference</a></a>
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        <div class="main">
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            <section id="Composite_Parts" class="js-scroll-step">
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                <div class="headline">
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                    Composite Parts
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                </div>
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                <p class="indent">We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is <span><a class="click_here" href="http://parts.igem.org/Part:BBa_K2623030">BBa_K2623030</a></span>, which links <span><a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a></span> and <span><a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a></span>, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatcher.</p>
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                <div class="table_container">
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                <table class="table">
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                    <tr><span>
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                        <th>Favorite</th>
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                        <th>Register Name</th>
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                        <th>Code used in Lab</th>
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                        <th>Description</th>
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                        <th>Types</th>
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                        <th>Lenth</th>
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                        <th>Designer</th>
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                    </tr>
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                    <tr>
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                        <td>R</td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623030">BBa_K2623030</a></td>
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                        <td>NG9-1</td>
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                        <td>A composite part linked by <a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a> and <a href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a>.</td>
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                        <td>Composite</td>
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                        <td>3410 bp</td>
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                        <td>Yang Liang</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623011">BBa_K2623011</a></td>
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                        <td>Kai 2</td>
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                        <td>Phosphorylation pathsway builder of Kai</td>
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                        <td>Composite</td>
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                        <td>4444 bp</td>
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                        <td>Gezhi Xiao</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623013">BBa_K2623013</a></td>
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                        <td>Kai 1 3</td>
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                        <td>Kai's phosphorylation core oscllator builder and SfYFP reportor for pKaiBC</td>
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                        <td>Composite</td>
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                        <td>3978 bp</td>
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                        <td>Hongling Liu</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623014">BBa_K2623014</a></td>
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                        <td>CAHS 1</td>
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                        <td>Cytosolic-abundant heat soluble protein "CAHS " (promoter, RBS, RFP, lacI and double terminator)</td>
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                        <td>Composite</td>
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                        <td>2891 bp</td>
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                        <td>Hongling Liu</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623015">BBa_K2623015</a></td>
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                        <td>SAHS 1</td>
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                        <td>Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, RFP and double terminator)</td>
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                        <td>Composite</td>
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                        <td>1425 bp</td>
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                        <td>Jun Dai</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623016">BBa_K2623016</a></td>
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                        <td>SAHS 2</td>
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                        <td>Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, amilCP and double terminator)</td>
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                        <td>Composite</td>
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                        <td>1431 bp</td>
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                        <td>Yuanjun Shang</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623021">BBa_K2623021</a></td>
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                        <td>NG1</td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> with GFP at its N-termini</td>
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                        <td>Reporter</td>
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                        <td>1657 bp</td>
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                        <td>Niangui Cai</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a></td>
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                        <td>NG2</td>
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                        <td>Bacterial outer membrane protein A (OmpA) fused with SpyTag at its C-termini</td>
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                        <td>Composite</td>
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                        <td>910 bp</td>
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                        <td>Qiupeng Wang</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623023">BBa_K2623023</a></td>
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                        <td>NG3</td>
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                        <td>Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini </td>
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                        <td>Reporter</td>
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                        <td>1645 bp</td>
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                        <td>Junhong Chen</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a></td>
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                        <td>NG4</td>
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                        <td>A non-GFP form of the <a href="http://parts.igem.org/Part:BBa_K2623023">BBa_K2623023</a></td>
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                        <td>Composite</td>
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                        <td>898 bp</td>
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                        <td>Jiyang Zheng</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a></td>
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                        <td>NG5</td>
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                        <td>Archaeal ribosomal protein protein L7Ae fused with SpyCatcher and mRFP1.</td>
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                        <td>Reporter</td>
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                        <td>2613 bp</td>
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                        <td>Ruofan Yang</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623026">BBa_K2623026</a></td>
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                        <td>NG6</td>
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                        <td>Archaeal ribosomal protein protein L7Ae fused with SpyCatcher.</td>
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                        <td>Composite</td>
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                        <td>2317 bp</td>
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                        <td>Yunyun Hu</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a></td>
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                        <td>NG8-1</td>
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                        <td>A composite part linked by <a href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> and <a href="http://parts.igem.org/Part:BBa_K2623025">BBa_K2623025</a></td>
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                        <td>Composite</td>
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                        <td>3410 bp</td>
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                        <td>Niangui Cai</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623029">BBa_K2623029</a></td>
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                        <td>NG8-2</td>
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                        <td>A composite part linked by <a href="http://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> and <a href="http://parts.igem.org/Part:BBa_K2623026">BBa_K2623026</a>.</td>
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                        <td>Composite</td>
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                        <td>2698 bp</td>
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                        <td>Ye Qiang</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623031">BBa_K2623031</a></td>
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                        <td>NG9-2</td>
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                        <td>A composite part linked by <a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a> and <a  class="click_here" href="http://parts.igem.org/Part:BBa_K2623026">BBa_K2623026</a></td>
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                        <td>Composite</td>
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                        <td>2698 bp</td>
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                        <td>Junhong Chen</td>
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                    </tr>
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                    <tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2623032">BBa_K2623032</a></td>
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                        <td>NG10</td>
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                        <td>A composite part linked by <a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a>, BBa_K2623026 and <a href="http://parts.igem.org/Part:BBa_K2623027">BBa_K2623027</a></td>
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                        <td>Composite</td>
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                        <td>4153 bp</td>
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                        <td>Niangui Cai</td>
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                    </tr>
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                </table>
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                </div>
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            </section>
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                    <li>Home</li>
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                    <li>Basic Part</li>
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                    <li>About us</li>
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                    <li>Composite Part</li>
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                    <li>Attribution</li>
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                    <li>Design</li>
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                    <li>Protocols</li>
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                    <li>Notebook</li>
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                    <li>Description</li>
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                    <li>Experiment</li>
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Latest revision as of 09:13, 17 October 2018

Team:XMU-China/Composite Parts - 2018.igem.org

Composite Parts
Composite Parts

We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is BBa_K2623030, which links BBa_K2623024 and BBa_K2623025, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatcher.

Favorite Register Name Code used in Lab Description Types Lenth Designer
R BBa_K2623030 NG9-1 A composite part linked by BBa_K2623024 and BBa_K2623025. Composite 3410 bp Yang Liang
BBa_K2623011 Kai 2 Phosphorylation pathsway builder of Kai Composite 4444 bp Gezhi Xiao
BBa_K2623013 Kai 1 3 Kai's phosphorylation core oscllator builder and SfYFP reportor for pKaiBC Composite 3978 bp Hongling Liu
BBa_K2623014 CAHS 1 Cytosolic-abundant heat soluble protein "CAHS " (promoter, RBS, RFP, lacI and double terminator) Composite 2891 bp Hongling Liu
BBa_K2623015 SAHS 1 Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, RFP and double terminator) Composite 1425 bp Jun Dai
BBa_K2623016 SAHS 2 Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, amilCP and double terminator) Composite 1431 bp Yuanjun Shang
BBa_K2623021 NG1 BBa_K2623022 with GFP at its N-termini Reporter 1657 bp Niangui Cai
BBa_K2623022 NG2 Bacterial outer membrane protein A (OmpA) fused with SpyTag at its C-termini Composite 910 bp Qiupeng Wang
BBa_K2623023 NG3 Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini Reporter 1645 bp Junhong Chen
BBa_K2623024 NG4 A non-GFP form of the BBa_K2623023 Composite 898 bp Jiyang Zheng
BBa_K2623025 NG5 Archaeal ribosomal protein protein L7Ae fused with SpyCatcher and mRFP1. Reporter 2613 bp Ruofan Yang
BBa_K2623026 NG6 Archaeal ribosomal protein protein L7Ae fused with SpyCatcher. Composite 2317 bp Yunyun Hu
BBa_K2623028 NG8-1 A composite part linked by BBa_K2623022 and BBa_K2623025 Composite 3410 bp Niangui Cai
BBa_K2623029 NG8-2 A composite part linked by BBa_K2623022 and BBa_K2623026. Composite 2698 bp Ye Qiang
BBa_K2623031 NG9-2 A composite part linked by BBa_K2623024 and BBa_K2623026 Composite 2698 bp Junhong Chen
BBa_K2623032 NG10 A composite part linked by BBa_K2623024, BBa_K2623026 and BBa_K2623027 Composite 4153 bp Niangui Cai
  • Home
  • Basic Part
  • About us
  • Composite Part
  • Attribution
  • Design
  • Modeling
  • Engagemen
  • Biosafety
  • Contribution
  • Collaboration
  • Protocols
  • Notebook
  • Description
  • Experiment