Difference between revisions of "Team:NAU-CHINA/Attributions"

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         <p class="sec-title">Attributions</p>
 
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            <p>We would like to thank those who have helped us in the competition. Their help and support for us to make our project a success.</p>
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            <h1>Team members </h1>           
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            <p>As the caption of NAU-CHINA 2018, main rsponsibilities of<b> Yaxuan Wu </b>are to design the project, experiments and interact with other iGEM teams. He also do some experiments. He preside over the bi-weekly group meeting, and check the team members' work at the meeting, and issue new tasks.</p>
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<p><b>Jitao Ge</b> participated in the construction of the syn-Notch transgenic plasmid and completed the design, registration, construction and sample submission of parts. He also participated in the completion of Safety Form, Human practice, notebook recording and wiki writing.  </p>
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<p><b>Yuan Yu</b> constructed plasmids in upstream circuit and did cell experiments to examine the effect of our system.</p>
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<p><b>Sheng Xu</b>
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designed most cloning strategies including the system of Recombinases and Recombinases with RDF .He also assisted in interlab.
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<p><b>Chunfeng Wang</b> constructed a portion of plasmids about the recombinase and recombinases with RDF , as well as three parts. She is also responsible for the pronuclear verification of recombinase.</p>
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<p><b>Chengzhu Fang</b> constructed plasmids, checked the problems in the laboratory, did experiments on cells, and participated in interlab study.</p>
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<p><b>Peikai Li</b> assisted with simple experimental operations and participated in the human practice activities. He also built a mathematical model to simulate the feasibility and stability of the path. By using different algorithms for calculation, he got the results.</p>
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<p><b>Yifei Ren</b> was responsible for human practice and wet lab. He planned the work arrangement of the whole human practice work, organized the popular science propaganda, interviews with researchers from the Chinese Academy of Sciences, interviews on citizens, campus lectures, and training in synthetic biology in high schools.  He also constructed a plasmid which can be used to verify the function of RDF.</p>
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<p><b>Zelan Huang</b> was mainly responsible for HP's various matters, and continuously carried out related activities and information collection, and timely gave feedback to the subject. </p>
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<p><b>Jiangyuan Liu</b> is responsible for subject crossing: removing the barrier between experiments and modeling.Wiki master.
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</p>
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<p><b>Siqi Wei</b> is responsible for the production and modification of wiki, collecting the process and result materials for it. Besides, her previous work focused on the wet lab.</p>
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<p><b>Yiheng Liu</b> participated in Interlab study, designed posters, PPTs and part of wiki elements</p>
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<p><b>Jiaxin Zhou</b> participated in the artwork of project, as well as the construction and verification of recombinase and RDf system.</p>
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<p><b>Hao Rong</b> was responsible for the software part and developed the application called iGEMCloud.</p>
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     <a href="https://2018.igem.org/Team:NAU-CHINA/Team_members">
 
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             <h1>XJTLU-CHINA</h1>
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             <h1>Teachers </h1>
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             <p><b>Prof. Xiong You</b> supported us in modeling.</p>         
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                    <a href="https://2018.igem.org/Team:XJTLU-CHINA/Collaborations "><img src="https://static.igem.org/mediawiki/2018/thumb/7/70/T--NAU-China--collaboration1.png/900px-T--NAU-China--collaboration1.png"
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            <p><b>Prof. Jiandong Jiang</b> supported us and provided laboratory, funds and other resources.</p>
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                    width="25%"  height="25%"></a>
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            <p><b>Prof. Xi Chen (Nanjing Agricultural University)</b> allowed us to use his lab to do some experiments.</p>
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                </figure>        
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            <p><b>Prof. Xi Chen (Nanjing University)</b> allowed us to use his lab space and arrange his student Xu Guo to help our cell experiments.</p>
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             <p><a href="https://2018.igem.org/Team:XJTLU-CHINA/Collaborations ">Xi'an Jiaotong-Liverpool University iGEM team</a> hoped that we completed the following descriptions, but their model design changed later, and this program may be abandoned by them.</p>
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             <p><b>Prof. Sheng Yang</b> allowed us to use his lab space and arrange his student Yingzhuo Wang to help us construct plasmids.</p>      
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             <p>XJTLU would like to complete a model describing exosome carrying mRNA into the BBB (blood-brain barrier). It is necessary to construct a relationship about the parameters. We assisted them in the construction of the third model. Our main work is to establish a mathematical model that calculates the effective residual amount of mRNA that reaches endothelial cells after t at a certain size. The method is to check the literature that you have found and the other party's literature to understand their model concept, analyze the main factors affecting the remaining amount: secretion loss and degeneration loss, then discuss the rationality of the model. </p>
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             <p>The overall purpose of their third model is to simulate the following figure. The purpose of simulating this figure is to obtain a range of effective values of mRNA that finally reaches the nerve cells by simulating the process by which the exosomes pass through the blood-brain barrier.</p>
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            <p><b>Prof. Jin Wang</b> for providing us with equipments and laboratory.</p>
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             <p><b>Prof. Wei Xuan</b> Supplied us with YFP gene.</p>
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            <p><b>Prof. Jingyu Lang</b> allowed us to use his lab space such as Cell Culture Room and gave us lots of advices for our project.</p>
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            <p><b>Associate Prof. Wei Lu</b> together with <b>Experimentalist Jun Chen</b>  gave great advice on how to manage and arrange a lab for maximum efficiency and mentorship on safety within the lab.</p>
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             <p><b>Prof. Jin Cui</b> supported us in funding.</p>  
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             <p><b>Teacher Xiaoyue Wang </b>for regularly checking on our work and giving very useful feedback.</p>
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            <p><b>Lecturer Jufang Wang</b> helped us to write wiki in order to eliminate errors in grammar and vocabulary. She also helped us on presentation and poster, makes them attractive and scientific.</p>
 
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            <h1>OUC-China </h1>
 
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                    <a href="https://2018.igem.org/Team:OUC-China/Collaborations "><img src="https://static.igem.org/mediawiki/2018/6/6b/T--NAU-China--collaboration_ouc.png"  width="20%"  height="20%"></a>
 
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            <p>We have contacted <a href="https://2018.igem.org/Team:OUC-China/Collaborations "> Ocean University of China</a> in the early stage to reach a cooperation. We will provide a PDF version of our Synthetic Biology comic book in our campus synthetic biology and iGEM publicity activities, which we will explain on the day of the event.</p>
 
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            <p>On September 28, 2018, the iGEM team of Nanjing Agricultural University and the Science Association of the College of Life Sciences jointly organized the iGEM science popularization activities on campus. The whole activity focused on “going to the laboratory – the daily life of the school team” and “going to the competition”. - Themes and Benefits, "Going Close to Dreams - Recruiting Talents" Three themes, using the panels to present synthetic biology knowledge, iGEM competition profiles and team composition, as well as new topics for this year's iGEM team.</p>
 
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            <p>The gifts of this event not only have the "transport RNA" keychain designed by our iGEM team, but also the cooperation support of synthetic biology comics designed by <a href="https://2018.igem.org/Team:OUC-China/Collaborations ">OUC</a>. Many students have come to look up and ask.</p>
 
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              <h1>Peking </h1>
 
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            <p><a href="https://2018.igem.org/Team:Peking/Collaborations">Peking University</a> provided our laboratory with the plasmid we needed.</p>
 
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              <h1>HZAU-China</h1>  
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            <h1>Advisors </h1>          
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              <figure>
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          <p><b>Long Yuan</b> is a Student advisor, primarily brainstormed while designing the project, preliminarily tested the upstream response with various elements. </p>
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                    <a href="https://2018.igem.org/Team:HZAU-China/Collaborations
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          <p><b>Xu Guo</b> is the helper of our cell experiments. He guides us on how to do cell experiments.</p>
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"><img src="https://static.igem.org/mediawiki/2018/a/ae/T--NAU-China--collaboration_5.jpeg"  width="33%"  height="33%"></a>
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            <p><a href="https://2018.igem.org/Team:HZAU-China/Collaborations
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">HZAU-China</a> has designed the OmpA-RGD protein to achieve this goal because of the need to enhance the targeting of engineered Salmonella they use. RGD is a well-studied tumor homing tripeptide that specifically binds to αvβ3 (αvβ3) integrin, which is widely overexpressed on cancer cells. So there is a cell line called MDA-MB-231 that shows the αvβ3 needed for their further targeting and safety experiments. So we helped them contact the channel from the Shanghai Institute of Biochemistry and Cell Biology to get the cell line.
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             <h1>CPU_CHINA</h1>
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             <h1>Other sources of help </h1>          
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            <figure>
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          <p><b>Yiming Dong</b> provided us with three plasmids containing recombinases.</p>
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                    <a href="https://2018.igem.org/Team:CPU_CHINA/Collaborations "><img src="https://static.igem.org/mediawiki/2018/0/0b/T--NAU-China--CPU.jpg"
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          <p>Students of <b>Prof. Ying Huang</b>，including<b> Yuhan Zhang</b> and <b>Sha Cheng</b> provided swingeing help in purification of EGFP in National Center for Protein Science Shanghai.</p>
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          <p>Students of <b>Prof. Xiong You</b>，including<b> Dongxu Diao</b> and <b>Siyao Liu</b> provided help in Algorithm design.</p>
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          <p><b>Qingyi Shen</b> dubbed for the App promotional video.</p>
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            <p>Based on the problems encountered in the advancement of the previous issues, we developed an APP in order to solve the problem of communication between the teams on the subject, real-time understanding of the dynamics of each team, and seeking help.</p>
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<p>The following is an introduction to our app:</p>
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<p>We developed iGEMCloud to solve some of iGEM's current problems, with immediacy, openness and interactivity. Currently, information about iGEM comes from many websites. They are scattered, chaotic, and unfriendly to individual users. So we created this app (until now, it is based on android, we hope to launch iOS version in the future), I hope it is an information collection platform, receiving the latest information of iGEM, this is a microblog user who allows everyone to personally or The form of the team is involved in the discussion and the community built for iGEMer around the world.</p>
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<p>In order to better get feedback from our app and improve accordingly to make it more suitable for team use, our ideas are more perfect. We contacted <a href="https://2018.igem.org/Team:CPU_CHINA/Collaborations ">China Pharmaceutical University</a> to help us test the software.</p>
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<p>They help us fix the software by sending post updates and more.</p>
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<p>Based on the posts they sent, we conducted another software fix upgrade.</p>
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<p>China Pharmaceutical University gave us affirmation and help, and put forward corresponding opinions on the user's feelings, etc., did not give us encouragement, but also let us have a direction for the improvement of APP in the future.</p>
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              <h1>XMU-China</h1>
 
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                    <a href="https://2018.igem.org/Team:XMU-China/Collaborations "><img src="https://static.igem.org/mediawiki/2018/thumb/5/51/T--NAU-China--XMU.png/1200px-T--NAU-China--XMU.png"  width="33%"  height="33%"></a>
 
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                </figure>
 
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            <p>On July 31, 2018, we met <a href="https://2018.igem.org/Team:XMU-China/Collaborations ">Xiamen University</a> in Taiwan and established a deep friendship. Since then, our two teams have exchanged and discussed "how to build a team." First we discussed "what kind of team should we build?" The key to team recruiting is to let them know what kind of team they will join. What is the team building for, what role will it be in the team, what should be done by itself, in order to join the team and How do you prepare and learn in this team? We think this is the key point for recruiting new ones. But at the same time, these should not be boring regulations, but should be integrated into the spirit, atmosphere, meaning, and our common goals of the entire team.</p>
 
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<p>Our previous methods were similar. We promoted through the seminars, conducted campus exhibitions, and let everyone communicate with us face to face. We can also understand your thoughts and questions more clearly. It is mainly to hold activities that can reach more people and raise awareness of iGEM.</p>
 
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<p>In the early stage, we discussed with the Xiamen University team about the norms and methods of the team selection process. Later, we also discussed how to train new players in the initial stage of team formation.</p>
 
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<p>In May 2018, we conducted the 2019 iGEM recruiting seminar. After the presentation, we conducted the first round of team formation through the QQ group, and released our training and selection items in it. Everyone entered different groups according to their intentions. After that, the relevant leaders in the 2018 team will be trained. In the early stage, I met with you through the group meeting, and arranged and taught the tasks according to different contents, and conducted an assessment between September and October 2018. Different groups determine the way of assessment according to needs. The number module is tested by some models, the art team is initially selected by some design tasks, and the other groups are also performing corresponding tasks.</p>
 
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<p>Throughout the training period, we also conducted unified training for the experimental part. At present, we are more likely to share the necessary bibliography, organize everyone to self-learn, and submit corresponding summary reports on a regular basis.</p>
 
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<p>We have exchanged information with Xiamen University this year. They have put forward some opinions on our training. During the communication process, we found that the selection methods between our two teams are different. They will be the newly selected players. After the determination, the training is carried out, and we are gradually training during the entire selection process, and through the step by step assessment. At the same time, it was found that a large amount of literature reading was started at the beginning, which is a bit difficult for the initial selection. It may be necessary to provide some help, such as some training in reading skills and reading focus. After the discussion, we conducted the previous literature learning training in the second phase of the selection, and found that the literature review written by the second phase of the team was more logical, and also able to better grasp the key points.</p>
 
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<p>Finally, we communicated with the daily management of the team, just like the problems encountered when starting the team. After entering the team, the basic aspects of the experimental part must be approved. After the preliminary determination of the project, each The tasks of the group are gradually becoming clearer. When we communicate with each other, we all have one thing in common. Most of us have a number of positions, which also strengthens the communication between groups. It shows that iGEM is a common combination, although there are different combinations. Division of labor, but everyone does not work separately, but is always closely linked.</p>
 
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<p>Through the exchanges with the iGEM team at Xiamen University, we have a better system in the whole construction process of the team, and we have more experience in the future recruiting, and all aspects are more organized.
 
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            <h1>Fudan </h1>
 
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            <p>We received the the plasmids with synnotch and surface-expressed EGFP from <a href="https://2018.igem.org/Team:Fudan/Collaborations">iGEM 2018 Fudan Team</a>, which constributed to the construction of our upstream circuit. </p>
 
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            <h1>ZJUT-China</h1>
 
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                  <a href="https://2018.igem.org/Team:ZJUT-China/Collaborations"> <img src="https://static.igem.org/mediawiki/2018/thumb/d/dd/T--NAU-China--collarZJUT.png/900px-T--NAU-China--collarZJUT.png"  width="20%"  height="20%"></a>
 
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            <p>By chance, we contacted <a href="https://2018.igem.org/Team:ZJUT-China/Collaborations">Zhejiang University of Technology</a> and hoped to communicate on the construction of the website. The two sides mainly conducted in-depth and multi-faceted exchanges on WIKI production and writing details, and also shared some problems they discovered during the construction process, reminding the other party not to make mistakes on this issue. Later, we discussed layout development, grammar optimization, and emerging Web development techniques.</p>
 
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<p>Throughout the construction of the entire webpage, with the exchanges between our two parties, there will be some places that have suddenly realized each other, and they are very rewarding and have established a profound team friendship.
 
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Revision as of 11:44, 17 October 2018

Template:2018_NAU-CHINA

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InterLab

Team

Attributions

We would like to thank those who have helped us in the competition. Their help and support for us to make our project a success.

Team members

As the caption of NAU-CHINA 2018, main rsponsibilities of Yaxuan Wu are to design the project, experiments and interact with other iGEM teams. He also do some experiments. He preside over the bi-weekly group meeting, and check the team members' work at the meeting, and issue new tasks.

Jitao Ge participated in the construction of the syn-Notch transgenic plasmid and completed the design, registration, construction and sample submission of parts. He also participated in the completion of Safety Form, Human practice, notebook recording and wiki writing.

Yuan Yu constructed plasmids in upstream circuit and did cell experiments to examine the effect of our system.

Sheng Xu designed most cloning strategies including the system of Recombinases and Recombinases with RDF .He also assisted in interlab.

Chunfeng Wang constructed a portion of plasmids about the recombinase and recombinases with RDF , as well as three parts. She is also responsible for the pronuclear verification of recombinase.

Chengzhu Fang constructed plasmids, checked the problems in the laboratory, did experiments on cells, and participated in interlab study.

Peikai Li assisted with simple experimental operations and participated in the human practice activities. He also built a mathematical model to simulate the feasibility and stability of the path. By using different algorithms for calculation, he got the results.

Yifei Ren was responsible for human practice and wet lab. He planned the work arrangement of the whole human practice work, organized the popular science propaganda, interviews with researchers from the Chinese Academy of Sciences, interviews on citizens, campus lectures, and training in synthetic biology in high schools. He also constructed a plasmid which can be used to verify the function of RDF.

Zelan Huang was mainly responsible for HP's various matters, and continuously carried out related activities and information collection, and timely gave feedback to the subject.

Jiangyuan Liu is responsible for subject crossing: removing the barrier between experiments and modeling.Wiki master.

Siqi Wei is responsible for the production and modification of wiki, collecting the process and result materials for it. Besides, her previous work focused on the wet lab.

Yiheng Liu participated in Interlab study, designed posters, PPTs and part of wiki elements

Jiaxin Zhou participated in the artwork of project, as well as the construction and verification of recombinase and RDf system.

Hao Rong was responsible for the software part and developed the application called iGEMCloud.

Teachers

Prof. Xiong You supported us in modeling.

Prof. Jiandong Jiang supported us and provided laboratory, funds and other resources.

Prof. Xi Chen (Nanjing Agricultural University) allowed us to use his lab to do some experiments.

Prof. Xi Chen (Nanjing University) allowed us to use his lab space and arrange his student Xu Guo to help our cell experiments.

Prof. Sheng Yang allowed us to use his lab space and arrange his student Yingzhuo Wang to help us construct plasmids.

Prof. Jin Wang for providing us with equipments and laboratory.

Prof. Wei Xuan Supplied us with YFP gene.

Prof. Jingyu Lang allowed us to use his lab space such as Cell Culture Room and gave us lots of advices for our project.

Associate Prof. Wei Lu together with Experimentalist Jun Chen gave great advice on how to manage and arrange a lab for maximum efficiency and mentorship on safety within the lab.

Prof. Jin Cui supported us in funding.

Teacher Xiaoyue Wang for regularly checking on our work and giving very useful feedback.

Lecturer Jufang Wang helped us to write wiki in order to eliminate errors in grammar and vocabulary. She also helped us on presentation and poster, makes them attractive and scientific.

Advisors

Long Yuan is a Student advisor, primarily brainstormed while designing the project, preliminarily tested the upstream response with various elements.

Xu Guo is the helper of our cell experiments. He guides us on how to do cell experiments.

Other sources of help

Yiming Dong provided us with three plasmids containing recombinases.

Students of Prof. Ying Huang，including Yuhan Zhang and Sha Cheng provided swingeing help in purification of EGFP in National Center for Protein Science Shanghai.

Students of Prof. Xiong You，including Dongxu Diao and Siyao Liu provided help in Algorithm design.

Qingyi Shen dubbed for the App promotional video.

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