Difference between revisions of "Team:NTHU Formosa/Parts"

Line 8: Line 8:
 
   <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/initial&action=raw&ctype=text/css">
 
   <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/initial&action=raw&ctype=text/css">
 
   <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/wordcss0&action=raw&ctype=text/css">
 
   <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/wordcss0&action=raw&ctype=text/css">
−
<link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/wordcss&action=raw&ctype=text/css">
+
  <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/wordcss&action=raw&ctype=text/css">
  
 
   <style>
 
   <style>
Line 32: Line 32:
  
 
     /* Table */
 
     /* Table */
 +
 
     table {
 
     table {
−
        font-family: "Josefin Sans","JosefinSans-Bold",NotoSansCJKtc-Regular,'Noto Sans',"微軟雅黑","Microsoft YaHei",Helvetica,sans-serif;
+
      font-family: "Josefin Sans", "JosefinSans-Bold", NotoSansCJKtc-Regular, 'Noto Sans', "微軟雅黑", "Microsoft YaHei", Helvetica, sans-serif;
−
        font-size: 22px;
+
      font-size: 22px;
−
    border-collapse: collapse;
+
      border-collapse: collapse;
−
    width: 100%;
+
      width: 100%;
 
     }
 
     }
  
−
     td, th {
+
     td,
−
    border: 1px solid #dddddd;
+
    th {
−
    text-align: left;
+
      border: 1px solid #dddddd;
−
    padding: 8px;
+
      text-align: left;
 +
      padding: 8px;
 
     }
 
     }
  
 
     tr:nth-child(even) {
 
     tr:nth-child(even) {
−
    background-color: #dddddd;
+
      background-color: #dddddd;
 
     }
 
     }
 +
 
     /* selected link */
 
     /* selected link */
  
Line 61: Line 64:
 
<!-- Main Section -->
 
<!-- Main Section -->
 
<div class="w3-container w3-padding-64 w3-center">
 
<div class="w3-container w3-padding-64 w3-center">
−
   <div class="w3-content"><br><br><br>
+
   <div class="w3-content">
−
     <h2 class="w3-wide" style="font-size:60px;">Parts&Component</h2>
+
    <br>
−
<br>
+
    <br>
−
<br>
+
    <br>
−
<br>
+
     <h2 class="w3-wide" style="font-size:60px;font-family:Quicksand;">Parts&Component</h2>
−
<br>
+
    <br>
 +
    <br>
 +
    <br>
 +
    <br>
 +
 
  
  
 +
    </p>
 +
    <!-- Table -->
  
−
</p>
+
    <body>
−
<!-- Table -->
+
−
<body>
+
  
  
  
−
<table>
+
      <table>
−
  <tr>
+
        <tr>
−
    <th>Name</th>
+
          <th>Name</th>
−
    <th>Type</th>
+
          <th>Type</th>
−
    <th>Description</th>
+
          <th>Description</th>
−
  </tr>
+
        </tr>
−
  <tr>
+
        <tr>
−
    <td><a href="http://parts.igem.org/Part:BBa_K2635000">BBa_K2635000</a></td>
+
          <td><a href="http://parts.igem.org/Part:BBa_K2635000">BBa_K2635000</a></td>
−
    <td>basic</td>
+
          <td>basic</td>
−
    <td>pTRE3G-mCherry</td>
+
          <td>pTRE3G-mCherry</td>
−
  </tr>
+
        </tr>
  
−
  <tr>
+
        <tr>
−
    <td><a href="http://parts.igem.org/Part:BBa_K2635001">BBa_K2635001</a></td>
+
          <td><a href="http://parts.igem.org/Part:BBa_K2635001">BBa_K2635001</a></td>
−
    <td>composite</td>
+
          <td>composite</td>
−
    <td>pCAG-GBP</td>
+
          <td>pCAG-GBP</td>
−
  </tr>
+
        </tr>
  
−
  <tr>
+
        <tr>
−
    <td><a href="http://parts.igem.org/Part:BBa_K2635002">BBa_K2635002</a></td>
+
          <td><a href="http://parts.igem.org/Part:BBa_K2635002">BBa_K2635002</a></td>
−
    <td>basic</td>
+
          <td>basic</td>
−
    <td>GBP N-terminal</td>
+
          <td>GBP N-terminal</td>
−
  </tr>
+
        </tr>
  
−
  <tr>
+
        <tr>
−
    <td><a href="http://parts.igem.org/Part:BBa_K2635003">BBa_K2635003</a></td>
+
          <td><a href="http://parts.igem.org/Part:BBa_K2635003">BBa_K2635003</a></td>
−
    <td>basic</td>
+
          <td>basic</td>
−
    <td>GBP C-terminal</td>
+
          <td>GBP C-terminal</td>
−
  </tr>
+
        </tr>
  
−
  <tr>
+
        <tr>
−
    <td><a href="http://parts.igem.org/Part:BBa_K2635004">BBa_K2635004</a></td>
+
          <td><a href="http://parts.igem.org/Part:BBa_K2635004">BBa_K2635004</a></td>
−
    <td>basic</td>
+
          <td>basic</td>
−
    <td>TEV N-terminal</td>
+
          <td>TEV N-terminal</td>
−
  </tr>
+
        </tr>
  
−
  <tr>
+
        <tr>
−
    <td><a href="http://parts.igem.org/Part:BBa_K2635005">BBa_K2635005</a></td>
+
          <td><a href="http://parts.igem.org/Part:BBa_K2635005">BBa_K2635005</a></td>
−
    <td>basic</td>
+
          <td>basic</td>
−
    <td>TEV C-terminal</td>
+
          <td>TEV C-terminal</td>
−
  </tr>
+
        </tr>
  
−
  <tr>
+
        <tr>
−
    <td><a href="http://parts.igem.org/Part:BBa_K2635006">BBa_K2635006</a></td>
+
          <td><a href="http://parts.igem.org/Part:BBa_K2635006">BBa_K2635006</a></td>
−
    <td>basic</td>
+
          <td>basic</td>
−
    <td>tTA </td>
+
          <td>tTA </td>
−
  </tr>
+
        </tr>
  
−
  <tr>
+
        <tr>
−
    <td><a href="http://parts.igem.org/Part:BBa_K2635007">BBa_K2635007</a></td>
+
          <td><a href="http://parts.igem.org/Part:BBa_K2635007">BBa_K2635007</a></td>
−
    <td>basic</td>
+
          <td>basic</td>
−
    <td>TEV cleavage site ENLYFQL</td>
+
          <td>TEV cleavage site ENLYFQL</td>
−
  </tr>
+
        </tr>
  
−
  <tr>
+
        <tr>
−
    <td><a href="http://parts.igem.org/Part:BBa_K2635008">BBa_K2635008</a></td>
+
          <td><a href="http://parts.igem.org/Part:BBa_K2635008">BBa_K2635008</a></td>
−
    <td>basic</td>
+
          <td>basic</td>
−
    <td>TEV cleavage site ENLYFQG</td>
+
          <td>TEV cleavage site ENLYFQG</td>
−
  </tr>
+
        </tr>
  
−
</table>
+
      </table>
  
−
</body>
+
    </body>
−
<br>
+
    <br>
−
<br>
+
    <br>
−
        <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/9/94/T--NTHU_Formosa--GBPpart.png" style="margin-left:auto;margin-right:auto;width:60%;" ;>
+
    <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/9/94/T--NTHU_Formosa--GBPpart.png" style="margin-left:auto;margin-right:auto;width:60%;" ;>
−
<br>
+
    <br>
−
<br>
+
    <br>
−
<br>
+
    <br>
  
−
        <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/a/a1/T--NTHU_Formosa--10107b.png" style="margin-left:auto;margin-right:auto;width:30%;" ;>
+
    <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/a/a1/T--NTHU_Formosa--10107b.png" style="margin-left:auto;margin-right:auto;width:30%;" ;>
−
<br>
+
    <br>
−
<br>
+
    <br>
−
<br>
+
    <br>
−
        <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/thumb/e/ed/T--NTHU_Formosa--10107c.png/798px-T--NTHU_Formosa--10107c.png" style="margin-left:auto;margin-right:auto;width:60%;" ;>
+
    <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/thumb/e/ed/T--NTHU_Formosa--10107c.png/798px-T--NTHU_Formosa--10107c.png" style="margin-left:auto;margin-right:auto;width:60%;" ;>
−
<br>
+
    <br>
−
<br>
+
    <br>
−
<br>
+
    <br>
  
−
        <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/8/8d/T--NTHU_Formosa--mcherrybiobrick.png" style="margin-left:auto;margin-right:auto;width:13%;" ;>
+
    <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/8/8d/T--NTHU_Formosa--mcherrybiobrick.png" style="margin-left:auto;margin-right:auto;width:13%;" ;>
  
 
   </div>
 
   </div>
Line 168: Line 175:
 
   <div class="modal-content">
 
   <div class="modal-content">
 
     <span class="close">&times;</span>
 
     <span class="close">&times;</span>
−
     <p>TEV proteases are the 27kDa catalytic domains of the NIa (Nuclear Inclusion a) protein encoded by Tobacco Etch Virus (TEV), where TEV proteases cut polyproteins into single proteins during biogenesis. TEV proteases recognize a linear epitope of the general form E-Xaa-Xaa-Y-Xaa-Q-(G/S) and cut the linkage between Q and G/S (Xaa can be freely substituted because variability in these positions was found in the natural cleavage sites of TEV’s polyprotein). Comparison of cleavage efficiency of different substract sequences demonstrated that “ENLYFQS” is the most efficient substrate sequence. The high-specificity of TEV’s cleavage makes it a popular tool for direct expression in living cells and protein purification.</p>
+
     <p>TEV proteases are the 27kDa catalytic domains of the NIa (Nuclear Inclusion a) protein encoded by Tobacco Etch Virus (TEV), where TEV proteases cut polyproteins into single proteins during biogenesis. TEV proteases recognize a linear epitope of the
 +
      general form E-Xaa-Xaa-Y-Xaa-Q-(G/S) and cut the linkage between Q and G/S (Xaa can be freely substituted because variability in these positions was found in the natural cleavage sites of TEV’s polyprotein). Comparison of cleavage efficiency of
 +
      different substract sequences demonstrated that “ENLYFQS” is the most efficient substrate sequence. The high-specificity of TEV’s cleavage makes it a popular tool for direct expression in living cells and protein purification.</p>
 
   </div>
 
   </div>
 
</div>
 
</div>

Revision as of 14:09, 17 October 2018




Parts&Component





Name Type Description
BBa_K2635000 basic pTRE3G-mCherry
BBa_K2635001 composite pCAG-GBP
BBa_K2635002 basic GBP N-terminal
BBa_K2635003 basic GBP C-terminal
BBa_K2635004 basic TEV N-terminal
BBa_K2635005 basic TEV C-terminal
BBa_K2635006 basic tTA
BBa_K2635007 basic TEV cleavage site ENLYFQL
BBa_K2635008 basic TEV cleavage site ENLYFQG