Difference between revisions of "Team:NTHU Formosa/InterLab"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>InterLab</h1>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
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For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab">InterLab study</a>, all work must be shown on this page.
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    <h2 class="w3-wide" style="font-size:60px;font-family:Quicksand;">2018 iGEM Interlab Study</h2><br>
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<p class="w3-center" style="font-size:40px;font-family:Quicksand;">Overall To-Do List</p><br>
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    <p class="w3-justify" style="font-size:25px;padding-left:150px;"><b>✔ Calibration 1：OD<sub>600</sub> Reference point - LUDOX Protocol</b></p>
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    <p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px;">1.Record settings for standard measurements</p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px;">2.Record measurements</p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px;">3.Fill out corresponding Excel sheet</p><br>
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    <p class="w3-justify" style="font-size:25px;padding-left:150px;"><b>✔ Calibration 2：​Particle Standard Curve - Microsphere Protocol</b></p>
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    <p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px;">1.Prepare Microsphere Stock Solution</p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px;">2.Serial dilution</p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px;">3.Measure Abs600 of all samples in instrument</p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px;">4.Record measurements</p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px;">5.Fill out corresponding Excel sheet</p><br>
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    <p class="w3-justify" style="font-size:25px;padding-left:150px;"><b>✔ Calibration 3：Fluorescence standard curve - Fluorescein Protocol </b></p>
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    <p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px;">1.Resuspend and dilute fluorescein</p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px;">2.Serial dilutions</p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px;">3.Measure fluorescence of all samples in instrument</p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px;">4.Record measurements</p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px;">5.Fill out corresponding Excel sheet</p><br>
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    <p class="w3-justify" style="font-size:25px;padding-left:150px"><b>✔ Cell Measurement protocol</b></p>
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<p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px">Day 1: Transform E.Coli DH5α with plasmids (all in pSB1C3)</p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px">Day 2: Pick 2 colonies per plate (5 mL LB medium + Chloramphenicol.  </p>
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    <p class="w3-justify" style="font-size:20px;padding-left:296px;">Grow 16-18 hours @ 37 C and 220 rpm</p>
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    <p class="w3-justify" style="font-size:20px;padding-left:240px">Day 3:
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      Dilution cultures in LB+Chloramphenicol <br>
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      <p class="w3-justify" style="font-size:20px;padding-left:304px;">Measure OD<sub>600</sub><br></p>
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      <p class="w3-justify" style="font-size:20px;padding-left:304px;">Further dilute<br></p>
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      <p class="w3-justify" style="font-size:20px;padding-left:304px;">Incubate<br></p>
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      <p class="w3-justify" style="font-size:20px;padding-left:304px;">Abs600 and fluorescence measurement<br></p>
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      <p class="w3-justify" style="font-size:20px;padding-left:304px;">Record measurements<br></p>
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      <p class="w3-justify" style="font-size:20px;padding-left:304px;">Fill out corresponding Excel sheet<br></p>
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    <p class="w3-justify" style="font-size:25px;padding-left:150px"><b>✔ Protocol：Colony Forming Units per 0.1 OD<sub>600</sub>  E.coli cultures</b></p>
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  <p class="w3-justify" style="font-size:20px;padding-left:150px"><br></p>
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      <p class="w3-justify" style="font-size:25px;padding-left:150px"><b>✔ To see the results, </b><a href="https://docs.google.com/spreadsheets/d/1GZ5xY4s5LGc7funlSDGDNl71Atd4TWjkDY37gka57aA/edit#gid=211336931"><src="https://docs.google.com/spreadsheets/d/1GZ5xY4s5LGc7funlSDGDNl71Atd4TWjkDY37gka57aA/edit#gid=211336931">click here</a>.</p>
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Latest revision as of 14:10, 17 October 2018




2018 iGEM Interlab Study


Overall To-Do List



✔ Calibration 1：OD600 Reference point - LUDOX Protocol


1.Record settings for standard measurements

2.Record measurements

3.Fill out corresponding Excel sheet


✔ Calibration 2：​Particle Standard Curve - Microsphere Protocol


1.Prepare Microsphere Stock Solution

2.Serial dilution

3.Measure Abs600 of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet


✔ Calibration 3：Fluorescence standard curve - Fluorescein Protocol


1.Resuspend and dilute fluorescein

2.Serial dilutions

3.Measure fluorescence of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet


✔ Cell Measurement protocol


Day 1: Transform E.Coli DH5α with plasmids (all in pSB1C3)

Day 2: Pick 2 colonies per plate (5 mL LB medium + Chloramphenicol.

Grow 16-18 hours @ 37 C and 220 rpm

Day 3: Dilution cultures in LB+Chloramphenicol

Measure OD600

Further dilute

Incubate

Abs600 and fluorescence measurement

Record measurements

Fill out corresponding Excel sheet


✔ Protocol：Colony Forming Units per 0.1 OD600 E.coli cultures


✔ To see the results, click here.