Difference between revisions of "Team:CCU Taiwan/Composite Part"
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</p> | </p> | ||
| − | <div id=" | + | <div id="Composite2" class="polaroid"> |
<img src="https://static.igem.org/mediawiki/parts/9/9e/T--CCU_Taiwan--114.png" style="width:100%"> | <img src="https://static.igem.org/mediawiki/parts/9/9e/T--CCU_Taiwan--114.png" style="width:100%"> | ||
<div class="container"> | <div class="container"> | ||
| − | <p>Figure | + | <p>Figure 2: The schematic structure of gene design- Px18.</p> |
</div> | </div> | ||
</div><br><br> | </div><br><br> | ||
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This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of peroxidase 16 gene sequence, FLAG-tag for Western Blot protein detection and 6xHis-tag for protein purification. | This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of peroxidase 16 gene sequence, FLAG-tag for Western Blot protein detection and 6xHis-tag for protein purification. | ||
| − | </p><br> <div id=" | + | </p><br> <div id="Composite3" class="polaroid"> |
<img src="https://static.igem.org/mediawiki/parts/1/1e/T--CCU_Taiwan--117.png" style="width:100%"> | <img src="https://static.igem.org/mediawiki/parts/1/1e/T--CCU_Taiwan--117.png" style="width:100%"> | ||
<div class="container"> | <div class="container"> | ||
| − | <p>Figure | + | <p>Figure 3: The schematic structure of gene design- Px16.</p> |
</div> | </div> | ||
</div><br> | </div><br> | ||
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<p class="second">Part construction in pGAPZ A:</p><br> | <p class="second">Part construction in pGAPZ A:</p><br> | ||
<div class="row"> | <div class="row"> | ||
| − | <div id=" | + | <div id="Composite4" class="polaroid"> |
<img src="https://static.igem.org/mediawiki/parts/f/f2/T--CCU_Taiwan--_pGAPZ_A_Lac1%2C_pGAPZ_A_Px16%2C_pGAPZ_A_Px18_Colony_PCR.jpeg" style="width:100%"> | <img src="https://static.igem.org/mediawiki/parts/f/f2/T--CCU_Taiwan--_pGAPZ_A_Lac1%2C_pGAPZ_A_Px16%2C_pGAPZ_A_Px18_Colony_PCR.jpeg" style="width:100%"> | ||
<div class="container"> | <div class="container"> | ||
| − | <p>Figure | + | <p>Figure 4: PCR result.<br> Well1: Marker/ Well2, 3, 4: Lac1 Colony 1, 2, 3/ Well5, 6, 7: Px16 Colony 1, 2, 3/ Well8, 9, 10: Px18 Colony 1, 2, 3/ Well11: pGAPZ A Colony |
<br> | <br> | ||
Insert long after PCR — pGAPZ A_Px16 :1377 bp/ pGAPZ A_Px18 :1359 bp/ pGAPZ A_Lac1 :2124bp | Insert long after PCR — pGAPZ A_Px16 :1377 bp/ pGAPZ A_Px18 :1359 bp/ pGAPZ A_Lac1 :2124bp | ||
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</div> | </div> | ||
</div> | </div> | ||
| − | <div id=" | + | <div id="Composite5" class="polaroid"> |
<img src="https://static.igem.org/mediawiki/parts/a/ae/T--CCU_Taiwan--_pSB1C3_Lac1%2C_pSB1C3_Px16%2C_pSB1C3_Px18_Miniprep_and_digest_with_EcoRI%2CAgeI.jpeg" style="width:100%"> | <img src="https://static.igem.org/mediawiki/parts/a/ae/T--CCU_Taiwan--_pSB1C3_Lac1%2C_pSB1C3_Px16%2C_pSB1C3_Px18_Miniprep_and_digest_with_EcoRI%2CAgeI.jpeg" style="width:100%"> | ||
<div class="container"> | <div class="container"> | ||
| − | <p>Figure | + | <p>Figure 5: Miniprep plasmid digest by EcoRI, PstI.<br>Well 1: Marker/ Well2, 3, 4: Plasmid of Lac1 colony1, 2, 3 after EcoRI, AgeI digest/ Well5, 6, 7: Plasmid of Px16 colony1, 2, 3 after EcoRI, AgeI digest/ Well8, 9, 10: Plasmid of Px18 colony1, 2, 3 after EcoRI, AgeI digest |
<br> | <br> | ||
Vector long after digest — pGAPZ A: 2884bp | Vector long after digest — pGAPZ A: 2884bp | ||
Revision as of 18:27, 17 October 2018
COMPOSITE PART
BBa_K2809011
laccase 1
We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809010.
This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of laccase gene sequence, HA tag for Western Blot protein detection and 6xHis-tag for protein purification.
Figure 1: The schematic structure of gene design- Lac1.
BBa_K2809021
peroxidase 18
We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809020.
This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of peroxidase 18 gene sequence, V5-tag for Western Blot protein detection and 6xHis-tag for protein purification.
Figure 2: The schematic structure of gene design- Px18.
BBa_K2809031
peroxidase 16
We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809030.
This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of peroxidase 16 gene sequence, FLAG-tag for Western Blot protein detection and 6xHis-tag for protein purification.
Figure 3: The schematic structure of gene design- Px16.
Part construction in pGAPZ A:
Figure 4: PCR result.
Well1: Marker/ Well2, 3, 4: Lac1 Colony 1, 2, 3/ Well5, 6, 7: Px16 Colony 1, 2, 3/ Well8, 9, 10: Px18 Colony 1, 2, 3/ Well11: pGAPZ A Colony
Insert long after PCR — pGAPZ A_Px16 :1377 bp/ pGAPZ A_Px18 :1359 bp/ pGAPZ A_Lac1 :2124bp
Figure 5: Miniprep plasmid digest by EcoRI, PstI.
Well 1: Marker/ Well2, 3, 4: Plasmid of Lac1 colony1, 2, 3 after EcoRI, AgeI digest/ Well5, 6, 7: Plasmid of Px16 colony1, 2, 3 after EcoRI, AgeI digest/ Well8, 9, 10: Plasmid of Px18 colony1, 2, 3 after EcoRI, AgeI digest
Vector long after digest — pGAPZ A: 2884bp
Insert long after digest — Px16: 1295bp/ Px18: 1277bp/ Lac1: 2042bp