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{{XMU-China}}
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                        <li class="Project">
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                            <a href="#" target="_blank">Project</a>
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                            <ul>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Description">Description</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Design">Design</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Results">Results</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Parts">Parts</a></li>
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                            </ul>
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                        </li>
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                    </ul>
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                    <ul class="second-nav">
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                        <li class="Hardware">
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                            <a href="#">Hardware</a>
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                            <ul>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Hardware">Overview</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Microfluidic_Chips">Microfluidic Chips</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Fluorescence_Detection">Fluorescence Detection</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Raspberry_Pi">Raspberry Pi</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Application">Application</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Software">Software</a></li>
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                                <li><a href="https://2018.igem.org/Team:XMU-China/Applied_Design">Product Design</a></li>
 +
                            </ul>
 +
                        </li>
 +
                        <li class="Model">
 +
                            <a href="#">Model</a>
 +
                            <ul>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Model#Summary">Summary</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Model#Thermodynamic_model">Thermodynamic Model</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Model#Fluid_dynamics_model">Fluid dynamics Model</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Model#Molecular_docking_model">Molecular Docking Model</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Model#The_dynamic_model">Derivation of Rate Equation</a></li>
 +
                            </ul>
 +
                        </li>
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                        <li class="Human_Practice">
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                            <a href="#">Social Works</a>
 +
                            <ul>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Human_Practices">Human Practice</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Public_Engagement">Engagement</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Entrepreneurship">Entrepreneurship</a></li>
 +
                            </ul>
 +
                        </li>
 +
                        <li class="Other_Works">
 +
                            <a href="#">Other Works</a>
 +
                            <ul>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/InterLab">InterLab</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Improve">Improve</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Safety">Safety</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Measurement">Measurement</a></li>
 +
                            </ul>
 +
                        </li>
 +
                        <li class="Notebook">
 +
                            <a href="#">Notebook</a>
 +
                            <ul>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Notebook">Notebook</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Experiments">Experiments</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Engineering">Engineering</a></li>
 +
                            </ul>
 +
                        </li>
 +
                        <li class="Team">
 +
                            <a href="#">Team</a>
 +
                            <ul>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Team">Members</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Attributions">Attributions</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/Judging">Judging</a></li>
 +
                                <li><a href="https://2018.igem.org/Team:XMU-China/After_iGEM">After iGEM</a></li>
 +
                            </ul>
 +
                        </li>
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                    </ul>
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                <div id="Team">
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                    <div class="nav-word">Team</div>
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                    <ul>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Team">Members</a></li>
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                        <li><a href="https://2018.igem.org/Team:XMU-China/Attributions">Attributions</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Judging">Judging</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/After_iGEM">After iGEM</a></li>
 +
                    </ul>
 +
                </div>
 +
                <div id="Notebook">
 +
                    <div class="nav-word">Notebook</div>
 +
                    <ul>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Notebook">Notebook</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Experiments">Experiments</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Engineering">Engineering</a></li>
 +
                    </ul>
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                </div>
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                <div id="Other_Works">
 +
                    <div class="nav-word">Other Works</div>
 +
                    <ul>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/InterLab">InterLab</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Improve">Improve</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Safety">Safety</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Measurement">Measurement</a></li>
 +
                    </ul>
 +
                </div>
 +
                <div id="Human_Practice">
 +
                    <div class="nav-word">Social Works</div>
 +
                    <ul>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Human_Practices">Human Practice</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Public_Engagement">Engagement</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Entrepreneurship">Entrepreneurship</a></li>
 +
                    </ul>
 +
                </div>
 +
                <div id="Model">
 +
                    <div class="nav-word">Model</div>
 +
                    <ul>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Model">Summary</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Model#Thermodynamic_model">Thermodynamic Model</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Model#Fluid_dynamics_model">Fluid dynamics Model</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Model#Molecular_docking_model">Molecular Docking Model</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Model#The_dynamic_model">Derivation of Rate Equation</a></li>
 +
                    </ul>
 +
                </div>
 +
                <div id="Hardwork">
 +
                    <div class="nav-word">Hardware</div>
 +
                    <ul>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Hardware">Overview</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Microfluidic_Chips">Microfluidic Chips</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Fluorescence_Detection">Fluorescence Detection</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Raspberry_Pi">Raspberry Pi</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Application">Application</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Software">Software</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Applied_Design">Product Design</a></li>
 +
                    </ul>
 +
                </div>
 +
                <div id="Project">
 +
                    <div class="nav-word">Project</div>
 +
                    <ul>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Description">Description</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Design">Design</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Results">Results</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
 +
                        <li><a href="https://2018.igem.org/Team:XMU-China/Parts">Parts</a></li>
 +
                    </ul>
 +
                </div>
 +
                <div id="jiannan">
 +
                    <a href="https://2018.igem.org/Team:XMU-China">
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                        <img src="https://static.igem.org/mediawiki/2018/6/6f/T--XMU-China--jiannanlogo.png">
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                    </a>
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                </div>
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            </div>
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 +
        <!-- 正文 -->
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                <div>Cell-free Systems for Disease Detection and Treatment</div>
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                <p>Fang: One Glance of Eternity.</p>
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                    </div>
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                                    <a href="https://2018.igem.org/Team:XMU-China/Model"><img src="https://static.igem.org/mediawiki/2018/e/e5/T--XMU-China--bannerbg4.png"></a>
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                    </div>
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                                    <a href="https://2018.igem.org/Team:XMU-China/Team"><img src="https://static.igem.org/mediawiki/2018/4/42/T--XMU-China--bannerbg5.png"></a>
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<h1>Project Description</h1>
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<p>Microorganisms are always interacting in nature, but in the laboratory it can be difficult to culture two species together in one medium. Mixed populations are difficult to maintain for a variety of reasons. For instance, a difference in growth rates can result in one population outcompeting the other. Our team hopes to use synthetic optogenetic systems to dynamically control and maintain the growth of two populations simultaneously.</p>
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<p>To control bacterial growth, our systems will target MetE, an enzyme needed for the synthesis of methionine. Since methionine is essential for protein synthesis, bacteria can only grow if they have the MetE gene or if their environment is supplemented with methionine. By placing MetE production under the control of an optogenetically-regulated promoter, we can tune the growth of our bacteria. In order to control two different bacterial populations independently, each promoter will be regulated by a distinct optogenetic system. The two systems we have chosen are CcaS/CcaR, which is activated by green light, and Opto-T7 RNA polymerase, which is activated by blue light. Thus, we can control growth of our two population by switching MetE production on and off with different lights. For example, shining blue light will increase growth of the Opto-T7 population.     </p>
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<p>Our system enables the growth of different microorganisms in the same culture. Hence, it opens several doors in biotech and research. It could improve the metabolic engineering of microbial populations used in the production of pharmaceuticals, biofuels, and other important materials. It could also allow researchers to explore complex interactions between microbes and investigate questions that could not previously be answered due to co-culturing limitations.</p>
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                                <div>Our Project</div>
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                                <p>This year team XMU-China developed cell-free systems to detect and treat diseases. Protein detection is unique and significant in biology fields, especially for the detection of protein biomarkers which produced by diseased cells. In order to overcome the deficiencies of traditional detection methods, we have developed <a class="click_here" href="https://2018.igem.org/Team:XMU-China/Description#ABCDsystem">an Aptamer Based Cell-free Detection system of protein.</a> </p>
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                                <p>After protein detection, we use outer-membrane vesicles <a class="click_here" href="https://2018.igem.org/Team:XMU-China/Description#OMVs">(OMVs)</a> to treat the diseased cells. We designed a system that has realized the efficient, customizable production of OMVs, which serves to encapsulate specific siRNA for disease treatment.</p>
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                                <p>To guarantee the practicability detection and treatment system, we also developed <a class="click_here" href="https://2018.igem.org/Team:XMU-China/Description#Supporting">TDPs supporting system</a> to store fragile chemicals or biological materials. </p>
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                                <p>To <a class="click_here" href="https://2018.igem.org/Team:XMU-China/Demonstrate">demonstrate</a> our project under realistic conditions, we designed a <a class="click_here" href="https://2018.igem.org/Team:XMU-China/Hardware">hardware</a> with matched <a class="click_here" href="https://2018.igem.org/Team:XMU-China/Software">software</a>. The design of ABCD system and microfluidic chips are guided by our <a class="click_here" href="https://2018.igem.org/Team:XMU-China/Model">models.</a></p>
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Latest revision as of 20:45, 17 October 2018

Team:XMU-China - 2018.igem.org

Cell-free Systems for Disease Detection and Treatment

Fang: One Glance of Eternity.

Our Project

This year team XMU-China developed cell-free systems to detect and treat diseases. Protein detection is unique and significant in biology fields, especially for the detection of protein biomarkers which produced by diseased cells. In order to overcome the deficiencies of traditional detection methods, we have developed an Aptamer Based Cell-free Detection system of protein.

After protein detection, we use outer-membrane vesicles (OMVs) to treat the diseased cells. We designed a system that has realized the efficient, customizable production of OMVs, which serves to encapsulate specific siRNA for disease treatment.

To guarantee the practicability detection and treatment system, we also developed TDPs supporting system to store fragile chemicals or biological materials.

To demonstrate our project under realistic conditions, we designed a hardware with matched software. The design of ABCD system and microfluidic chips are guided by our models.

Contact Us