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− | <link rel="stylesheet" | + | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/Lato&action=raw&ctype=text/css" /> |
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<link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/CSS&action=raw&ctype=text/css"> | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/CSS&action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/CSS2&action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/initial&action=raw&ctype=text/css"> | ||
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+ | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/wordcss&action=raw&ctype=text/css"> | ||
+ | |||
<style> | <style> | ||
/* unvisited link */ | /* unvisited link */ | ||
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color: hotpink; | color: hotpink; | ||
cursor: pointer; | cursor: pointer; | ||
+ | } | ||
+ | |||
+ | |||
+ | /* Table */ | ||
+ | |||
+ | table { | ||
+ | font-family: "Josefin Sans", "JosefinSans-Bold", NotoSansCJKtc-Regular, 'Noto Sans', "微軟雅黑", "Microsoft YaHei", Helvetica, sans-serif; | ||
+ | font-size: 22px; | ||
+ | border-collapse: collapse; | ||
+ | width: 100%; | ||
+ | } | ||
+ | |||
+ | td, | ||
+ | th { | ||
+ | border: 1px solid #dddddd; | ||
+ | text-align: left; | ||
+ | padding: 8px; | ||
+ | } | ||
+ | |||
+ | tr:nth-child(even) { | ||
+ | background-color: #dddddd; | ||
} | } | ||
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<!-- Main Section --> | <!-- Main Section --> | ||
− | <div class="w3-container | + | <div class="w3-container w3-padding-64 w3-center"> |
<div class="w3-content"> | <div class="w3-content"> | ||
− | <h2 class="w3-wide"> | + | <br> |
− | <p | + | <br> |
+ | <br> | ||
+ | <h2 class="w3-wide" style="font-size:60px;font-family:Quicksand;">Parts & Component</h2> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
+ | |||
+ | |||
+ | </p> | ||
+ | <!-- Table --> | ||
+ | |||
+ | <body> | ||
+ | |||
+ | |||
+ | |||
+ | <table> | ||
+ | <tr> | ||
+ | <th><font face="Quicksand">Name</font></th> | ||
+ | <th><font face="Quicksand">Type</font></th> | ||
+ | <th><font face="Quicksand">Description</font></th> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><a | ||
+ | href="http://parts.igem.org/Part:BBa_K2635000"> | ||
+ | <font face="Quicksand">BBa_K2635000</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">pTRE3G-mCherry</font></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a | ||
+ | href="http://parts.igem.org/Part:BBa_K2635001"> | ||
+ | <font face="Quicksand">BBa_K2635001</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">composite</font></td> | ||
+ | <td><font face="Quicksand">pCAG-GBP</font></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a | ||
+ | href="http://parts.igem.org/Part:BBa_K2635002"> | ||
+ | <font face="Quicksand">BBa_K2635002</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">GBP N-terminal</font></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a | ||
+ | href="http://parts.igem.org/Part:BBa_K2635003"> | ||
+ | <font face="Quicksand">BBa_K2635003</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">GBP C-terminal</font></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a | ||
+ | href="http://parts.igem.org/Part:BBa_K2635004"> | ||
+ | <font face="Quicksand">BBa_K2635004</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">TEV N-terminal</font></td> | ||
+ | </tr> | ||
− | < | + | <tr> |
− | + | <td><a | |
− | < | + | href="http://parts.igem.org/Part:BBa_K2635005"> |
− | + | <font face="Quicksand">BBa_K2635005</font> | |
− | < | + | </a></td> |
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">TEV C-terminal</font></td> | ||
+ | </tr> | ||
− | < | + | <tr> |
− | + | <td><a | |
+ | href="http://parts.igem.org/Part:BBa_K2635006"> | ||
+ | <font face="Quicksand">BBa_K2635006</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">tTA</font></td> | ||
+ | </tr> | ||
− | </ | + | <tr> |
− | + | <td><a | |
+ | href="http://parts.igem.org/Part:BBa_K2635007"> | ||
+ | <font face="Quicksand">BBa_K2635007</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">TEV cleavage site ENLYFQL</font></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a | ||
+ | href="http://parts.igem.org/Part:BBa_K2635008"> | ||
+ | <font face="Quicksand">BBa_K2635008</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">TEV cleavage site ENLYFQG</font></td> | ||
+ | </tr> | ||
+ | |||
+ | </table> | ||
+ | </body> | ||
+ | <br> | ||
+ | <br> | ||
+ | <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/9/94/T--NTHU_Formosa--GBPpart.png" style="margin-left:auto;margin-right:auto;width:60%;" ;> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/a/a1/T--NTHU_Formosa--10107b.png" style="margin-left:auto;margin-right:auto;width:30%;" ;> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/thumb/e/ed/T--NTHU_Formosa--10107c.png/798px-T--NTHU_Formosa--10107c.png" style="margin-left:auto;margin-right:auto;width:60%;" ;> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/2/2c/T--NTHU_Formosa--BIOBRICK_03_03.png" style="margin-left:auto;margin-right:auto;width:13%;" ;> | ||
</div> | </div> | ||
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<div class="modal-content"> | <div class="modal-content"> | ||
<span class="close">×</span> | <span class="close">×</span> | ||
− | <p>TEV proteases are the 27kDa catalytic domains of the NIa (Nuclear Inclusion a) protein encoded by Tobacco Etch Virus (TEV), where TEV proteases cut polyproteins into single proteins during biogenesis. TEV proteases recognize a linear epitope of the general form E-Xaa-Xaa-Y-Xaa-Q-(G/S) and cut the linkage between Q and G/S (Xaa can be freely substituted because variability in these positions was found in the natural cleavage sites of TEV’s polyprotein). Comparison of cleavage efficiency of different substract sequences demonstrated that “ENLYFQS” is the most efficient substrate sequence. The high-specificity of TEV’s cleavage makes it a popular tool for direct expression in living cells and protein purification.</p> | + | <p>TEV proteases are the 27kDa catalytic domains of the NIa (Nuclear Inclusion a) protein encoded by Tobacco Etch Virus (TEV), where TEV proteases cut polyproteins into single proteins during biogenesis. TEV proteases recognize a linear epitope of the |
+ | general form E-Xaa-Xaa-Y-Xaa-Q-(G/S) and cut the linkage between Q and G/S (Xaa can be freely substituted because variability in these positions was found in the natural cleavage sites of TEV’s polyprotein). Comparison of cleavage efficiency of | ||
+ | different substract sequences demonstrated that “ENLYFQS” is the most efficient substrate sequence. The high-specificity of TEV’s cleavage makes it a popular tool for direct expression in living cells and protein purification.</p> | ||
</div> | </div> | ||
</div> | </div> |
Latest revision as of 00:55, 18 October 2018
Parts & Component
Name | Type | Description |
---|---|---|
BBa_K2635000 | basic | pTRE3G-mCherry |
BBa_K2635001 | composite | pCAG-GBP |
BBa_K2635002 | basic | GBP N-terminal |
BBa_K2635003 | basic | GBP C-terminal |
BBa_K2635004 | basic | TEV N-terminal |
BBa_K2635005 | basic | TEV C-terminal |
BBa_K2635006 | basic | tTA |
BBa_K2635007 | basic | TEV cleavage site ENLYFQL |
BBa_K2635008 | basic | TEV cleavage site ENLYFQG |