Difference between revisions of "Team:NTHU Formosa/Parts"

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{{NTHU_Formosa}}
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{{NTHU_Formosa}} {{NTHU_Formosa/NavBar}}
 
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  <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/Lato&action=raw&ctype=text/css" />
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  <style>
<h1>Parts</h1>
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    /* unvisited link */
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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    a {
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<h3>Note</h3>
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    }
<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h3>Adding parts to the registry</h3>
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    table {
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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    td,
<div class="button_link">
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    th {
<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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ADD PARTS
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</a>
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</head>
  
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<div class="highlight decoration_A_full">
 
<h3>Inspiration</h3>
 
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
  
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
<ul>
 
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
</ul>
 
</div>
 
</div>
 
  
  
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    <br>
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    <br>
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    <br>
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    <h2 class="w3-wide" style="font-size:60px;font-family:Quicksand;">Parts & Component</h2>
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    <br>
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    <br>
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    <br>
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    <br>
  
  
  
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    </p>
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    <!-- Table -->
  
<div class="column full_size">
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    <body>
  
<h3>What information do I need to start putting my parts on the Registry?</h3>
 
<p>The information needed to initially create a part on the Registry is:</p>
 
<ul>
 
<li>Part Name</li>
 
<li>Part type</li>
 
<li>Creator</li>
 
<li>Sequence</li>
 
<li>Short Description (60 characters on what the DNA does)</li>
 
<li>Long Description (Longer description of what the DNA does)</li>
 
<li>Design considerations</li>
 
</ul>
 
  
<p>
 
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
  
</div>
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      <table>
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        <tr>
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          <th><font face="Quicksand">Name</font></th>
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          <th><font face="Quicksand">Type</font></th>
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          <th><font face="Quicksand">Description</font></th>
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        </tr>
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        <tr>
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          <td><a
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href="http://parts.igem.org/Part:BBa_K2635000">
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          <font face="Quicksand">BBa_K2635000</font>
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            </a></td>
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          <td><font face="Quicksand">basic</font></td>
 +
          <td><font face="Quicksand">pTRE3G-mCherry</font></td>
 +
        </tr>
  
 +
        <tr>
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          <td><a
 +
href="http://parts.igem.org/Part:BBa_K2635001">
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          <font face="Quicksand">BBa_K2635001</font>
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            </a></td>
 +
          <td><font face="Quicksand">composite</font></td>
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          <td><font face="Quicksand">pCAG-GBP</font></td>
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        </tr> 
  
<div class="clear extra_space"></div>
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        <tr>
<div class="line_divider"></div>
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          <td><a
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href="http://parts.igem.org/Part:BBa_K2635002">
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          <font face="Quicksand">BBa_K2635002</font>
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            </a></td>
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          <td><font face="Quicksand">basic</font></td>
 +
          <td><font face="Quicksand">GBP N-terminal</font></td>
 +
        </tr>
  
<div class="column full_size">
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        <tr>
<h3>Part Table </h3>
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          <td><a
 +
href="http://parts.igem.org/Part:BBa_K2635003">
 +
          <font face="Quicksand">BBa_K2635003</font>
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            </a></td>
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          <td><font face="Quicksand">basic</font></td>
 +
          <td><font face="Quicksand">GBP C-terminal</font></td>
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        </tr>
  
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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        <tr>
 +
          <td><a  
 +
href="http://parts.igem.org/Part:BBa_K2635004">
 +
          <font face="Quicksand">BBa_K2635004</font>
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            </a></td>
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          <td><font face="Quicksand">basic</font></td>
 +
          <td><font face="Quicksand">TEV N-terminal</font></td>
 +
        </tr>
  
</html>
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        <tr>
<groupparts>iGEM18 NTHU_Formosa</groupparts>
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          <td><a
<html>
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href="http://parts.igem.org/Part:BBa_K2635005">
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          <font face="Quicksand">BBa_K2635005</font>
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            </a></td>
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          <td><font face="Quicksand">basic</font></td>
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          <td><font face="Quicksand">TEV C-terminal</font></td>
 +
        </tr>
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 +
        <tr>
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          <td><a
 +
href="http://parts.igem.org/Part:BBa_K2635006">
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          <font face="Quicksand">BBa_K2635006</font>
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            </a></td>
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          <td><font face="Quicksand">basic</font></td>
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          <td><font face="Quicksand">tTA</font></td>
 +
        </tr>
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 +
        <tr>
 +
          <td><a
 +
href="http://parts.igem.org/Part:BBa_K2635007">
 +
          <font face="Quicksand">BBa_K2635007</font>
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            </a></td>
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          <td><font face="Quicksand">basic</font></td>
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          <td><font face="Quicksand">TEV cleavage site ENLYFQL</font></td>
 +
        </tr>
 +
     
 +
        <tr>
 +
          <td><a
 +
href="http://parts.igem.org/Part:BBa_K2635008">
 +
          <font face="Quicksand">BBa_K2635008</font>
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            </a></td>
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          <td><font face="Quicksand">basic</font></td>
 +
          <td><font face="Quicksand">TEV cleavage site ENLYFQG</font></td>
 +
        </tr>       
 +
       
 +
      </table>
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    </body>
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    <br>
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    <br>
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    <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/9/94/T--NTHU_Formosa--GBPpart.png" style="margin-left:auto;margin-right:auto;width:60%;" ;>
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    <br>
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    <br>
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    <br>
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    <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/a/a1/T--NTHU_Formosa--10107b.png" style="margin-left:auto;margin-right:auto;width:30%;" ;>
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    <br>
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    <br>
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    <br>
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    <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/thumb/e/ed/T--NTHU_Formosa--10107c.png/798px-T--NTHU_Formosa--10107c.png" style="margin-left:auto;margin-right:auto;width:60%;" ;>
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    <br>
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    <br>
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    <br>
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 +
    <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/2/2c/T--NTHU_Formosa--BIOBRICK_03_03.png" style="margin-left:auto;margin-right:auto;width:13%;" ;>
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    <p>TEV proteases are the 27kDa catalytic domains of the NIa (Nuclear Inclusion a) protein encoded by Tobacco Etch Virus (TEV), where TEV proteases cut polyproteins into single proteins during biogenesis. TEV proteases recognize a linear epitope of the
 +
      general form E-Xaa-Xaa-Y-Xaa-Q-(G/S) and cut the linkage between Q and G/S (Xaa can be freely substituted because variability in these positions was found in the natural cleavage sites of TEV’s polyprotein). Comparison of cleavage efficiency of
 +
      different substract sequences demonstrated that “ENLYFQS” is the most efficient substrate sequence. The high-specificity of TEV’s cleavage makes it a popular tool for direct expression in living cells and protein purification.</p>
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Latest revision as of 00:55, 18 October 2018




Parts & Component





Name Type Description
BBa_K2635000 basic pTRE3G-mCherry
BBa_K2635001 composite pCAG-GBP
BBa_K2635002 basic GBP N-terminal
BBa_K2635003 basic GBP C-terminal
BBa_K2635004 basic TEV N-terminal
BBa_K2635005 basic TEV C-terminal
BBa_K2635006 basic tTA
BBa_K2635007 basic TEV cleavage site ENLYFQL
BBa_K2635008 basic TEV cleavage site ENLYFQG