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− | {{NTHU_Formosa}} | + | {{NTHU_Formosa}} {{NTHU_Formosa/NavBar}} |
<html> | <html> | ||
+ | <head> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/Lato&action=raw&ctype=text/css" /> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/CSS&action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/CSS2&action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/initial&action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/wordcss0&action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:NTHU_Formosa/wordcss&action=raw&ctype=text/css"> | ||
− | < | + | <style> |
− | + | /* unvisited link */ | |
− | + | ||
− | + | ||
− | + | ||
− | + | a { | |
− | + | color: #0645AD; | |
− | + | } | |
− | + | ||
− | + | ||
− | + | ||
− | + | /* visited link */ | |
− | + | ||
− | + | ||
+ | a:visited { | ||
+ | color: green; | ||
+ | } | ||
+ | /* mouse over link */ | ||
+ | a:hover { | ||
+ | color: hotpink; | ||
+ | cursor: pointer; | ||
+ | } | ||
− | + | /* Table */ | |
− | + | ||
− | + | table { | |
− | + | font-family: "Josefin Sans", "JosefinSans-Bold", NotoSansCJKtc-Regular, 'Noto Sans', "微軟雅黑", "Microsoft YaHei", Helvetica, sans-serif; | |
+ | font-size: 22px; | ||
+ | border-collapse: collapse; | ||
+ | width: 100%; | ||
+ | } | ||
− | + | td, | |
− | + | th { | |
− | + | border: 1px solid #dddddd; | |
− | + | text-align: left; | |
− | + | padding: 8px; | |
− | + | } | |
− | + | tr:nth-child(even) { | |
− | + | background-color: #dddddd; | |
+ | } | ||
+ | /* selected link */ | ||
+ | a:active { | ||
+ | color: blue; | ||
+ | } | ||
+ | </style> | ||
+ | </head> | ||
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− | |||
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− | |||
− | <div class=" | + | <!-- Main Section --> |
+ | <div class="w3-container w3-padding-64 w3-center"> | ||
+ | <div class="w3-content"> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <h2 class="w3-wide" style="font-size:60px;font-family:Quicksand;">Parts & Component</h2> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | </p> | ||
+ | <!-- Table --> | ||
− | < | + | <body> |
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− | |||
− | </ | + | <table> |
+ | <tr> | ||
+ | <th><font face="Quicksand">Name</font></th> | ||
+ | <th><font face="Quicksand">Type</font></th> | ||
+ | <th><font face="Quicksand">Description</font></th> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><a | ||
+ | href="http://parts.igem.org/Part:BBa_K2635000"> | ||
+ | <font face="Quicksand">BBa_K2635000</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">pTRE3G-mCherry</font></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a | ||
+ | href="http://parts.igem.org/Part:BBa_K2635001"> | ||
+ | <font face="Quicksand">BBa_K2635001</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">composite</font></td> | ||
+ | <td><font face="Quicksand">pCAG-GBP</font></td> | ||
+ | </tr> | ||
− | < | + | <tr> |
− | < | + | <td><a |
− | < | + | href="http://parts.igem.org/Part:BBa_K2635002"> |
+ | <font face="Quicksand">BBa_K2635002</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">GBP N-terminal</font></td> | ||
+ | </tr> | ||
− | < | + | <tr> |
− | < | + | <td><a |
+ | href="http://parts.igem.org/Part:BBa_K2635003"> | ||
+ | <font face="Quicksand">BBa_K2635003</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">GBP C-terminal</font></td> | ||
+ | </tr> | ||
− | < | + | <tr> |
+ | <td><a | ||
+ | href="http://parts.igem.org/Part:BBa_K2635004"> | ||
+ | <font face="Quicksand">BBa_K2635004</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">TEV N-terminal</font></td> | ||
+ | </tr> | ||
− | </ | + | <tr> |
− | < | + | <td><a |
− | < | + | href="http://parts.igem.org/Part:BBa_K2635005"> |
+ | <font face="Quicksand">BBa_K2635005</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">TEV C-terminal</font></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a | ||
+ | href="http://parts.igem.org/Part:BBa_K2635006"> | ||
+ | <font face="Quicksand">BBa_K2635006</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">tTA</font></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a | ||
+ | href="http://parts.igem.org/Part:BBa_K2635007"> | ||
+ | <font face="Quicksand">BBa_K2635007</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">TEV cleavage site ENLYFQL</font></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a | ||
+ | href="http://parts.igem.org/Part:BBa_K2635008"> | ||
+ | <font face="Quicksand">BBa_K2635008</font> | ||
+ | </a></td> | ||
+ | <td><font face="Quicksand">basic</font></td> | ||
+ | <td><font face="Quicksand">TEV cleavage site ENLYFQG</font></td> | ||
+ | </tr> | ||
+ | |||
+ | </table> | ||
+ | |||
+ | </body> | ||
+ | <br> | ||
+ | <br> | ||
+ | <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/9/94/T--NTHU_Formosa--GBPpart.png" style="margin-left:auto;margin-right:auto;width:60%;" ;> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
+ | <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/a/a1/T--NTHU_Formosa--10107b.png" style="margin-left:auto;margin-right:auto;width:30%;" ;> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/thumb/e/ed/T--NTHU_Formosa--10107c.png/798px-T--NTHU_Formosa--10107c.png" style="margin-left:auto;margin-right:auto;width:60%;" ;> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
+ | <img class="w3-round-large" src="https://static.igem.org/mediawiki/2018/2/2c/T--NTHU_Formosa--BIOBRICK_03_03.png" style="margin-left:auto;margin-right:auto;width:13%;" ;> | ||
+ | |||
+ | </div> | ||
</div> | </div> | ||
+ | <!-- Trigger/Open The Modal --> | ||
+ | <!-- <a id="myLink"><u>Open Modal</u></a> --> | ||
+ | |||
+ | <!-- The Modal Box--> | ||
+ | <div id="myModal" class="modal"> | ||
+ | <!-- Modal content --> | ||
+ | <div class="modal-content"> | ||
+ | <span class="close">×</span> | ||
+ | <p>TEV proteases are the 27kDa catalytic domains of the NIa (Nuclear Inclusion a) protein encoded by Tobacco Etch Virus (TEV), where TEV proteases cut polyproteins into single proteins during biogenesis. TEV proteases recognize a linear epitope of the | ||
+ | general form E-Xaa-Xaa-Y-Xaa-Q-(G/S) and cut the linkage between Q and G/S (Xaa can be freely substituted because variability in these positions was found in the natural cleavage sites of TEV’s polyprotein). Comparison of cleavage efficiency of | ||
+ | different substract sequences demonstrated that “ENLYFQS” is the most efficient substrate sequence. The high-specificity of TEV’s cleavage makes it a popular tool for direct expression in living cells and protein purification.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- Modal script, DO NOT MODIFY!!! --> | ||
+ | <script> | ||
+ | // Get the modal | ||
+ | var modal = document.getElementById('myModal'); | ||
+ | // Get the hyperlink | ||
+ | var link = document.getElementById("myLink"); | ||
+ | // // When the user clicks the hyperlink, open the modal | ||
+ | link.onclick = function() { | ||
+ | modal.style.display = "block"; | ||
+ | } | ||
+ | // Get the <span> element that closes the modal | ||
+ | var span = document.getElementsByClassName("close")[0]; | ||
+ | // When the user clicks on <span> (x), close the modal | ||
+ | span.onclick = function() { | ||
+ | modal.style.display = "none"; | ||
+ | } | ||
+ | // When the user clicks anywhere outside of the modal, close it | ||
+ | window.onclick = function(event) { | ||
+ | if (event.target == modal) { | ||
+ | modal.style.display = "none"; | ||
+ | } | ||
+ | } | ||
+ | </script> | ||
</html> | </html> |
Latest revision as of 00:55, 18 October 2018
Parts & Component
Name | Type | Description |
---|---|---|
BBa_K2635000 | basic | pTRE3G-mCherry |
BBa_K2635001 | composite | pCAG-GBP |
BBa_K2635002 | basic | GBP N-terminal |
BBa_K2635003 | basic | GBP C-terminal |
BBa_K2635004 | basic | TEV N-terminal |
BBa_K2635005 | basic | TEV C-terminal |
BBa_K2635006 | basic | tTA |
BBa_K2635007 | basic | TEV cleavage site ENLYFQL |
BBa_K2635008 | basic | TEV cleavage site ENLYFQG |