Difference between revisions of "Team:XMU-China/Notebook"

@@ Line 33: / Line 33: @@
      <!-- windows phone 点击无高光 -->
      <title>Team:XMU-China/Notebook - 2018.igem.org</title>
-     <link rel="stylesheet" href="css/notebook.css">
+     <link rel="stylesheet" href="css/engineering.css">
      <link rel="stylesheet" href="css/font.css">
      <link href="http://cdn.bootcss.com/font-awesome/4.7.0/css/font-awesome.min.css" rel="stylesheet">
-     <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/font?action=raw&ctype=text/css"> -->
+     <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/font?action=raw&ctype=text/css">
      <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/cover?action=raw&ctype=text/css">
      <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/footer?action=raw&ctype=text/css">
      <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/nav?action=raw&ctype=text/css">
      <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/nav_mobile?action=raw&ctype=text/css">
      <link rel="stylesheet" href="https://2018.igem.org/Team:XMU-China/css/engineering?action=raw&ctype=text/css">
      <script src="js/jquery-1.11.0.min.js" type="text/javascript"></script>
-     <script type="text/javascript" src="js/jquery.SuperSlide2.1.2.js"></script>
+     <!-- <script type="text/javascript" src="js/jquery.SuperSlide2.1.2.js"></script>  -->
      <script src="https://2018.igem.org/Team:XMU-China/js/time_line?action=raw&ctype=text/javascript"></script>
 </head>
@@ Line 58: / Line 58: @@
                                  <li><a href="https://2018.igem.org/Team:XMU-China/Description">Description</a></li>
                                  <li><a href="https://2018.igem.org/Team:XMU-China/Design">Design</a></li>
-                                <li><a href="https://2018.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
                                  <li><a href="https://2018.igem.org/Team:XMU-China/Results">Results</a></li>
+                                <li><a href="https://2018.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
                                  <li><a href="https://2018.igem.org/Team:XMU-China/Parts">Parts</a></li>
                              </ul>
@@ Line 69: / Line 69: @@
                              <ul>
                                  <li><a href="https://2018.igem.org/Team:XMU-China/Hardware">Overview</a></li>
-                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Microfluidic_Chips">Microfluidic chips</a></li>
+                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Microfluidic_Chips">Microfluidic Chips</a></li>
-                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Fluorescenc_Detection">Fluorescence Detection</a></li>
+                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Fluorescence_Detection">Fluorescence Detection</a></li>
-                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Straberry_Pi">Straberry Pi</a></li>
+                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Raspberry_Pi">Raspberry Pi</a></li>
-                                 <li><a href="https://2018.igem.org/Team:XMU-China/Applied_Design">Applied Design</a></li>
+                                 <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Application">Application</a></li>
-                                 <li><a href="https://2018.igem.org/Team:XMU-China/Software">APP</a></li>
+                                 <li><a href="https://2018.igem.org/Team:XMU-China/Software">Software</a></li>
+                                <li><a href="https://2018.igem.org/Team:XMU-China/Applied_Design">Product Design</a></li>
                              </ul>
                          </li>
@@ Line 79: / Line 80: @@
                              <a href="#">Model</a>
                              <ul>
-                                 <li><a href="https://2018.igem.org/Team:XMU-China/Model">Overview</a></li>
+                                 <li><a href="https://2018.igem.org/Team:XMU-China/Model#Summary">Summary</a></li>
+                                <li><a href="https://2018.igem.org/Team:XMU-China/Model#Thermodynamic_model">Thermodynamic Model</a></li>
+                                <li><a href="https://2018.igem.org/Team:XMU-China/Model#Fluid_dynamics_model">Fluid dynamics Model</a></li>
+                                <li><a href="https://2018.igem.org/Team:XMU-China/Model#Molecular_docking_model">Molecular Docking Model</a></li>
+                                <li><a href="https://2018.igem.org/Team:XMU-China/Model#The_dynamic_model">Derivation of Rate Equation</a></li>
                              </ul>
                          </li>
                          <li class="Human_Practice">
-                             <a href="#">Human Practice</a>
+                             <a href="#">Social Works</a>
                              <ul>
-                                 <li><a href="https://2018.igem.org/Te
+                                 <li><a href="https://2018.igem.org/Team:XMU-China/Human_Practices">Human Practice</a></li>
-                                am:XMU-China/Human_Practices">Overview</a></li>
-                                <li><a href="https://2018.igem.org/Team:XMU-China/HP/Silver">Silver</a></li>
-                                <li><a href="https://2018.igem.org/Team:XMU-China/HP/Gold_Integrated">Gold</a></li>
                                  <li><a href="https://2018.igem.org/Team:XMU-China/Public_Engagement">Engagement</a></li>
                                  <li><a href="https://2018.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li>
@@ Line 108: / Line 110: @@
                                  <li><a href="https://2018.igem.org/Team:XMU-China/Notebook">Notebook</a></li>
                                  <li><a href="https://2018.igem.org/Team:XMU-China/Experiments">Experiments</a></li>
+                                <li><a href="https://2018.igem.org/Team:XMU-China/Engineering">Engineering</a></li>
                              </ul>
                          </li>
@@ Line 116: / Line 119: @@
                                  <li><a href="https://2018.igem.org/Team:XMU-China/Attributions">Attributions</a></li>
                                  <li><a href="https://2018.igem.org/Team:XMU-China/Judging">Judging</a></li>
+                                <li><a href="https://2018.igem.org/Team:XMU-China/After_iGEM">After iGEM</a></li>
                              </ul>
                          </li>
@@ Line 124: / Line 128: @@
          </header>
      </div>
-    <!-- <script type="text/javascript" src="js/hc-mobile-nav.js"></script> -->
-    <!-- <script type="text/javascript" src="js/hc-mobile-button.js"></script> -->
      <script src="https://2018.igem.org/Team:XMU-China/js/hc-mobile-nav?action=raw&ctype=text/javascript"></script>
      <div class="header">
@@ Line 140: / Line 142: @@
                          <li><a href="https://2018.igem.org/Team:XMU-China/Attributions">Attributions</a></li>
                          <li><a href="https://2018.igem.org/Team:XMU-China/Judging">Judging</a></li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/After_iGEM">After iGEM</a></li>
                      </ul>
                  </div>
                  <div id="Notebook">
-                     <div>
+                     <div class="nav-word">Notebook</div>
-                        <div class="nav-word">Notebook</a></div>
-                    </div>
                      <ul>
                          <li><a href="https://2018.igem.org/Team:XMU-China/Notebook">Notebook</a></li>
                          <li><a href="https://2018.igem.org/Team:XMU-China/Experiments">Experiments</a></li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Engineering">Engineering</a></li>
                      </ul>
                  </div>
@@ Line 161: / Line 163: @@
                  </div>
                  <div id="Human_Practice">
-                     <div class="nav-word">Human Practice</div>
+                     <div class="nav-word">Social Works</div>
                      <ul>
-                         <li><a href="https://2018.igem.org/Team:XMU-China/Human_Practices">Overview</a></li>
+                         <li><a href="https://2018.igem.org/Team:XMU-China/Human_Practices">Human Practice</a></li>
-                        <li><a href="https://2018.igem.org/Team:XMU-China/HP/Silver">Silver</a></li>
-                        <li><a href="https://2018.igem.org/Team:XMU-China/HP/Gold_Integrated">Gold</a></li>
                          <li><a href="https://2018.igem.org/Team:XMU-China/Public_Engagement">Engagement</a></li>
                          <li><a href="https://2018.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li>
@@ Line 174: / Line 174: @@
                      <div class="nav-word">Model</div>
                      <ul>
-                         <li><a href="https://2018.igem.org/Team:XMU-China/Model">Overview</a></li>
+                         <li><a href="https://2018.igem.org/Team:XMU-China/Model">Summary</a></li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Model#Thermodynamic_model">Thermodynamic Model</a></li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Model#Fluid_dynamics_model">Fluid dynamics Model</a></li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Model#Molecular_docking_model">Molecular Docking Model</a></li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Model#The_dynamic_model">Derivation of Rate Equation</a></li>
                      </ul>
                  </div>
@@ Line 181: / Line 185: @@
                      <ul>
                          <li><a href="https://2018.igem.org/Team:XMU-China/Hardware">Overview</a></li>
-                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Microfluidic_Chips">Microfluidic chips</a></li>
+                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Microfluidic_Chips">Microfluidic Chips</a></li>
-                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Fluorescenc_Detection">Fluorescence Detection</a></li>
+                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Fluorescence_Detection">Fluorescence Detection</a></li>
-                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware/Straberry_Pi">Straberry Pi</a></li>
+                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Raspberry_Pi">Raspberry Pi</a></li>
-                         <li><a href="https://2018.igem.org/Team:XMU-China/Applied_Design">Applied Design</a></li>
+                         <li><a href="https://2018.igem.org/Team:XMU-China/Hardware#Application">Application</a></li>
-                         <li><a href="https://2018.igem.org/Team:XMU-China/Software">APP</a></li>
+                         <li><a href="https://2018.igem.org/Team:XMU-China/Software">Software</a></li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Applied_Design">Product Design</a></li>
                      </ul>
                  </div>
@@ Line 193: / Line 198: @@
                          <li><a href="https://2018.igem.org/Team:XMU-China/Description">Description</a></li>
                          <li><a href="https://2018.igem.org/Team:XMU-China/Design">Design</a></li>
-                        <li><a href="https://2018.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
                          <li><a href="https://2018.igem.org/Team:XMU-China/Results">Results</a></li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
                          <li><a href="https://2018.igem.org/Team:XMU-China/Parts">Parts</a></li>
                      </ul>
@@ Line 215: / Line 220: @@
                      <p class="right_space"></p>
                  </div>
-                 <div class="parHd clearfix notebook" id="engineering">
+                 <div class="parHd clearfix notebook" >
                      <div class="limited_framework">
                          <ul>
@@ Line 234: / Line 239: @@
                              <li><span></span>Sep Wk 4</li>
                              <li><span></span>Oct Wk 1</li>
                              <li><span></span>Oct Wk 2</li>
+                            <li><span></span>Oct Wk 3</li>
                          </ul>
                          <a class="sPrev"><img src="https://static.igem.org/mediawiki/2018/5/59/T--XMU-China--timeline_left.png"></a>
@@ Line 283: / Line 289: @@
                          <h1>Jul Wk 4</h1>
                          <p>– Interlab experiments.</p>
+                        <p>– Verified the activity of EpCAM.</p>
+                        <p class="F3"><img src="https://static.igem.org/mediawiki/2018/2/25/T--XMU-China--notebook-abcd-1.jpg"></p>
                      </div>
                      <div class="slideBox" style="display: none;">
@@ Line 288: / Line 297: @@
                          <p>– The genetic circuit of the Kai began to be built.</p>
                      </div>
-                     <div class="slideBox " style="display: none;">
+                     <div class="slideBox" style="display: none;">
                          <h1>Aug Wk 2</h1>
-                         <p class="left_align">– <a href="http://parts.igem.org/Part:BBa_K2623015">BBa_K2623015</a>constructed.</p>
+                         <p>– <a href="http://parts.igem.org/Part:BBa_K2623015">BBa_K2623015</a> constructed.</p>
                          <p class="dipic_3">
                              <img src="https://static.igem.org/mediawiki/2018/7/72/T--XMU-China--BBa_K2623015.png">
                              <img src="https://static.igem.org/mediawiki/2018/a/a9/T--XMU-China--BBa_K2623015_1.jpeg">
                          </p>
-                             <p class="left_align">
+                             <p>
                                  - <a href="http://parts.igem.org/Part:BBa_K2623009">BBa_K2623009</a>constructed.
                              </p>
@@ Line 301: / Line 310: @@
                                  <img src="https://static.igem.org/mediawiki/2018/1/17/T--XMU-China--BBa_K2623009.png">
                          </p>
-                                 <p class="left_align">– <a href="http://parts.igem.org/Part:BBa_K2623016">BBa_K2623016</a>constructed.
+                                 <p>– <a href="http://parts.igem.org/Part:BBa_K2623016">BBa_K2623016</a>constructed.
                                  </p>
                                  <p class="dipic_4">
@@ Line 307: / Line 316: @@
                                      <img src="https://static.igem.org/mediawiki/2018/9/98/T--XMU-China--BBa_K2623016_1.jpeg">
                          </p>
-                                     <p class="left_align">- The genetic circuit of SAHS was completed and protein validation began.
+                                     <p>- The genetic circuit of SAHS was completed and protein validation began.
                                      </p>
                      </div>
                      <div class="slideBox" style="display: none;">
                          <h1>Aug Wk 3</h1>
-                         <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_K2623001">BBa_K2623001</a> constructed.</p>
+                         <p>- <a href="http://parts.igem.org/Part:BBa_K2623001">BBa_K2623001</a> constructed.</p>
                          <p class="F8">
                              <img src="https://static.igem.org/mediawiki/2018/8/8a/T--XMU-China--BBa_K2623001.png">
                          </p>
-                             <p class="left_align">- <a href="ttp://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> and <a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a> constructed.
+                             <p>- <a href="ttp://parts.igem.org/Part:BBa_K2623022">BBa_K2623022</a> and <a href="http://parts.igem.org/Part:BBa_K2623024">BBa_K2623024</a> constructed.
                              </p>
                              <p class="F25">
                                  <img src="https://static.igem.org/mediawiki/2018/c/c7/T--XMU-China--BBa_K2623022_4.jpeg">
                          </p>
-                                 <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_I13401">BBa_I13401</a> constructed to get the GFP reporter.
+                                 <p>- <a href="http://parts.igem.org/Part:BBa_I13401">BBa_I13401</a> constructed to get the GFP reporter.
                                  </p>
                                  <p class="F25">
@@ Line 329: / Line 338: @@
                      <div class="slideBox" style="display: none;">
                          <h1>Aug Wk 4</h1>
-                         <p class="left_align">- <a href="ttp://parts.igem.org/Part:BBa_K2623021">BBa_K2623021</a> and <a href="http://parts.igem.org/Part:BBa_K2623023">BBa_K2623023</a> constructed.</p>
+                         <p>- <a href="ttp://parts.igem.org/Part:BBa_K2623021">BBa_K2623021</a> and <a href="http://parts.igem.org/Part:BBa_K2623023">BBa_K2623023</a> constructed.</p>
                          <p class="F25">
                              <img src="https://static.igem.org/mediawiki/2018/9/9a/T--XMU-China--BBa_K2623021_3.jpeg">
                          </p>
-                             <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_K2623026">BBa_K2623026</a> constructed.</p>
+                             <p>- <a href="http://parts.igem.org/Part:BBa_K2623026">BBa_K2623026</a> constructed.</p>
                              <p class="F25">
                                  <img src="https://static.igem.org/mediawiki/2018/7/7c/T--XMU-China--BBa_K2623026.jpeg">
                          </p>
-                                - Aptamer and ssDNA successfully combined.
-                                <p class="F25">
+                                     <p>- <a href="http://parts.igem.org/Part:BBa_K2623002">BBa_K2623002</a> constructed.</p>
-                                    <img src="https://static.igem.org/mediawiki/2018/9/93/T--XMU-China--ssDNA-Aptamer.jpeg">
-                        </p>
-                                     <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_K2623002">BBa_K2623002</a> constructed.</p>
                                      <p class="F8">
                                          <img src="https://static.igem.org/mediawiki/2018/d/d7/T--XMU-China--BBa_K2623002.png">
                          </p>
-                                         <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_K2623003">BBa_K2623003</a> constructed.</p>
+                                         <p>- <a href="http://parts.igem.org/Part:BBa_K2623003">BBa_K2623003</a> constructed.</p>
                                          <p class="F8">
                                              <img src="https://static.igem.org/mediawiki/2018/1/16/T--XMU-China--BBa_K2623003.png">
                          </p>
+                        <p>– Verified that SYL3C aptamer could combine with 10nt complementary chain successfully.</p>
+                        <p class="F25"><img src="https://static.igem.org/mediawiki/2018/5/59/T--XMU-China--notebook-abcd-2.jpg"></p>
                      </div>
                      <div class="slideBox" style="display: none;">
@@ Line 359: / Line 367: @@
                      <div class="slideBox" style="display: none;">
                          <h1>Sep Wk 2</h1>
-                         <p class="left_align">- GFP on <a href="ttp://parts.igem.org/Part:BBa_K2623021">BBa_K2623021</a> and <a href="http://parts.igem.org/Part:BBa_K2623023">BBa_K2623023</a> successfully expressed.</p>
+                         <p>- GFP on <a href="ttp://parts.igem.org/Part:BBa_K2623021">BBa_K2623021</a> and <a href="http://parts.igem.org/Part:BBa_K2623023">BBa_K2623023</a> successfully expressed.</p>
                          <p class="dipic_4">
                              <img src="https://static.igem.org/mediawiki/2018/e/e0/T--XMU-China--BBa_K2623021.jpeg">
                              <img src="https://static.igem.org/mediawiki/2018/f/f6/T--XMU-China--BBa_K2623023.jpeg">
                          </p>
-                             <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_K2623004">BBa_K2623004</a> constructed.</p>
+                             <p>- <a href="http://parts.igem.org/Part:BBa_K2623004">BBa_K2623004</a> constructed.</p>
-                             <p class="F25">
+                             <p class="F8">
                                  <img src="https://static.igem.org/mediawiki/2018/c/cd/T--XMU-China--BBa_K2623004.png">
                          </p>
-                                 <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_K2623005">BBa_K2623005</a> constructed.</p>
+                                 <p>- <a href="http://parts.igem.org/Part:BBa_K2623005">BBa_K2623005</a> constructed.</p>
-                                 <p class="F25">
+                                 <p class="F8">
                                      <img src="https://static.igem.org/mediawiki/2018/d/dd/T--XMU-China--BBa_K2623005.png">
                          </p>
-                                     <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_K2623006">BBa_K2623006</a> constructed.</p>
+                                     <p>- <a href="http://parts.igem.org/Part:BBa_K2623006">BBa_K2623006</a> constructed.</p>
-                                     <p class="F25">
+                                     <p class="F8">
                                          <img src="https://static.igem.org/mediawiki/2018/e/ef/T--XMU-China--BBa_K2623006.png">
                          </p>
-                                         <p class="left_align">- First time successful purification of high-purity SAHS protein.</p>
+                                         <p>- First time successful purification of high-purity SAHS protein.</p>
-                                         <p class="F25">
+                                         <p class="F8">
                                              <img src="https://static.igem.org/mediawiki/2018/f/f0/T--XMU-China--SAHS_protein.png">
                          </p>
-                                             <p class="left_align">- Coating experiment on microfluidic chips succeeded. <br>
+                                             <p>- Coating experiment on microfluidic chips succeeded. <br>
                                                  - Started bacterial freeze-drying experiment.
                                              </p>
@@ Line 386: / Line 394: @@
                      <div class="slideBox" style="display: none;">
                          <h1>Sep Wk 3</h1>
-                         <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_E1010"> BBa_E1010</a> constructed to get the mRFP reporter.</p>
+                         <p>- <a href="http://parts.igem.org/Part:BBa_E1010"> BBa_E1010</a> constructed to get the mRFP reporter.</p>
                          <p class="F25">
                              <img src="https://static.igem.org/mediawiki/2018/f/fb/T--XMU-China--BBa_E1010.jpeg">
                          </p>
-                             <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_K2623011"> BBa_K2623011</a> constructed.
+                             <p>- <a href="http://parts.igem.org/Part:BBa_K2623011"> BBa_K2623011</a> constructed.
-                                 <p class="F25">
+                                 <p class="F8">
                                      <img src="https://static.igem.org/mediawiki/2018/8/8b/T--XMU-China--BBa_K2623011.png">
                          </p>
-                                     <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_K2623007"> BBa_K2623007</a> constructed.
+                                     <p>- <a href="http://parts.igem.org/Part:BBa_K2623007"> BBa_K2623007</a> constructed.
-                                         <p class="F25">
+                                         <p class="F8">
                                              <img src="https://static.igem.org/mediawiki/2018/9/92/T--XMU-China--BBa_K2623007.png">
                          </p>
-                                             <p class="left_align">- Competition experiment succeeded.<br>
+                                             <p>- Competition experiment succeeded.<br>
                                                  - Started protein freeze-drying experiment.
                                              </p>
+                                            <p>– Verified the feasibility of “competition method” successfully.</p>
+                                            <p class="F3"><img src="https://static.igem.org/mediawiki/2018/b/bd/T--XMU-China--notebook-abcd-3.jpg"></p>
                      </div>
                      <div class="slideBox" style="display: none;">
                          <h1>Sep Wk 4</h1>
-                         <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_K2623025"> BBa_K2623025</a> constructed.
+                         <p>- <a href="http://parts.igem.org/Part:BBa_K2623025"> BBa_K2623025</a> constructed.
                              <p class="F25">
                                  <img src="https://static.igem.org/mediawiki/2018/7/77/T--XMU-China--BBa_K2623025.jpeg">
                          </p>
-                                 <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_K2623008"> BBa_K2623008</a> constructed.
+                                 <p>- <a href="http://parts.igem.org/Part:BBa_K2623008"> BBa_K2623008</a> constructed.
-                                     <p class="F25">
+                                     <p class="F8">
                                          <img src="https://static.igem.org/mediawiki/2018/6/60/T--XMU-China--BBa_K2623008.png">
                          </p>
-                                         <P class="left_align">- Started try genetic recombination experiment. </P>
+                                         <P>- Started try genetic recombination experiment. </P>
                      </div>
                      <div class="slideBox" style="display: none;">
                          <h1>Oct Wk 1</h1>
-                         <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_K2623027"> BBa_K2623027</a> constructed.
+                         <p>- <a href="http://parts.igem.org/Part:BBa_K2623027"> BBa_K2623027</a> constructed.
                              <p class="F25">
                                  <img src="https://static.igem.org/mediawiki/2018/4/48/T--XMU-China--BBa_K2623027.jpeg">
                          </p>
-                                 <p class="left_align">- <a href="http://parts.igem.org/Part:BBa_K2623013"> BBa_K2623013</a> constructed.
+                                 <p>- <a href="http://parts.igem.org/Part:BBa_K2623013"> BBa_K2623013</a> constructed.
                                      <p class="F25">
                                          <img src="https://static.igem.org/mediawiki/2018/c/cb/T--XMU-China--BBa_K2623013.png">
                          </p>
-                                         <p class="left_align">- NG2+5, NG2+6, NG4+5, NG4+6 constructed.
+                                         <p>- NG2+5, NG2+6, NG4+5, NG4+6 constructed.
                                          </p>
+                                        <p>– Verified that As Cas12a could be activated.</p>
+                                        <p class="F3"><img src="https://static.igem.org/mediawiki/2018/5/58/T--XMU-China--notebook-abcd-4.jpg"></p>
                      </div>
                      <div class="slideBox" style="display: none;">
                          <h1>Oct Wk 2</h1>
-                         <p>详细信息</p>
+                         <p>-
-                     </div>
+                            <a href="http://parts.igem.org/Part:BBa_K2623028">BBa_K2623028</a>,
+                            <a href="http://parts.igem.org/Part:-BBa_K2623029">BBa_K2623029</a>,
+                            <a href="http://parts.igem.org/Part:-BBa_K2623030">BBa_K2623030</a>,
+                            <a href="http://parts.igem.org/Part:-BBa_K2623031">BBa_K2623031</a> successfully constructed  and the fluorescence intensity of OMVs isolated from E.coli containing these four parts were detected by <a href="http://xmyan.xmu.edu.cn/">flow cytometer</a></p>
+                        <p class="F25">
+                        	<img src="https://static.igem.org/mediawiki/2018/8/89/T--XMU-China--Oct_wk2.png">
+                        </p>
+                        <p>– Verified the successful combination between SYL3C aptamer and EpCAM.</p>
+                        <p class="F2">
+                        	<img src="https://static.igem.org/mediawiki/2018/f/f5/T--XMU-China--notebook-abcd-5.jpg">
+                        </p>
+                        <p>– Transferred molecular signal to fluorescence signal with DNase Alert.</p>
+                        <p class="F2"><img src="https://static.igem.org/mediawiki/2018/4/43/T--XMU-China--notebook-abcd-6.jpg"></p>
+                    </div>
+                    <div class="slideBox" style="display: none;">
+                        <h1>Oct Wk 3</h1>
+                        <p>– Verified the transfer from protein signal to the fluorescent one successfully</p>
+                        <p class="F25">
+                            <img src="https://static.igem.org/mediawiki/2018/5/5e/T--XMU-China--notebook-abcd-7.png">
+                        </p>
+                     </div>
                  </div>
              </div>
@@ Line 439: / Line 473: @@
          <div class="footer">
              <div class="footer_top">
-                <img src="https://static.igem.org/mediawiki/2018/3/3d/T--XMU-China--xmu_is_different.png">
+                    <img src="https://static.igem.org/mediawiki/2018/3/3d/T--XMU-China--xmu_is_different.png">
-                <ul>
+                    <ul>
-                    <li>Home</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China">Home</a></a></li>
-                    <li>Basic Part</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Model">Model</a></li>
-                    <li>About us</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Parts">Parts</a></li>
-                    <li>Composite Part</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Entrepreneurship">Entrepreneurship</a></li>
-                    <li>Attribution</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Attributions">Attributions</a></li>
-                </ul>
+                    </ul>
-                <ul>
+                    <ul>
-                    <li>Design</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Design">Design</a></li>
-                    <li>Modeling</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Hardware">Hardware</a></li>
-                    <li>Engagemen</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Human_Practices">Human Practices</a></li>
-                    <li>Biosafety</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Notebook">Notebook</a></li>
-                    <li>Contribution</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Judging">Judging</a></li>
-                </ul>
+                    </ul>
-                <ul>
+                    <ul>
-                    <li>Collaboration</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Results">Results</a></li>
-                    <li>Protocols</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Software">Software</a></li>
-                    <li>Notebook</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Collaborations">Collaborations</a></li>
-                    <li>Description</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/Members">Members</a></li>
-                    <li>Experiment</li>
+                        <li><a href="https://2018.igem.org/Team:XMU-China/After_iGEM">After iGEM</a></li>
-                </ul>
+                    </ul>
-                <div class="clear"></div>
+                </div>
-            </div>
              <div class="bottom"></div>
          </div>

Latest revision as of 01:41, 18 October 2018

Team:XMU-China/Notebook - 2018.igem.org

Project
- Description
- Design
- Results
- Demonstrate
- Parts

Hardware
Model
Social Works
Other Works
- InterLab
- Improve
- Safety
- Measurement
Notebook
Team

Team

Members
Attributions
Judging
After iGEM

Notebook

Notebook
Experiments
Engineering

Other Works

InterLab
Improve
Safety
Measurement

Social Works

Human Practice
Engagement
Collaborations
Entrepreneurship

Model

Summary
Thermodynamic Model
Fluid dynamics Model
Molecular Docking Model
Derivation of Rate Equation

Hardware

Overview
Microfluidic Chips
Fluorescence Detection
Raspberry Pi
Application
Software
Product Design

Project

Description
Design
Results
Demonstrate
Parts

Notebook

Mar
Apr
May
Jun Wk 1-3
Jun Wk 4
Jul Wk 3
Jul Wk 4
Aug Wk 1
Aug Wk 2
Aug Wk 3
Aug Wk 4
Sep Wk 1
Sep Wk 2
Sep Wk 3
Sep Wk 4
Oct Wk 1
Oct Wk 2
Oct Wk 3

Mar

- Our team was formally founded.
- All student members learned the fundamental knowledge of molecular cloning and iGEM.
- All student members looked through the previous excellent projects and learned the basic notion of synthetic biology and iGEM.

Apr

- We were divided into four groups and started to brainstorm separately (and we reported each’s progress every weekend).
- Our team was formally accepted by iGEM.

May

- Our formal project was confirmed.
- The pre-experiment plan was confirmed.
- The division of labor was confirmed.

Jun Wk1-3

-All student members learned laboratory fundamental qualities and received molecular cloning operation training.

Jun Wk 4

- 2018 DNA Distribution Kit Plates were received.
- We transformed the biobricks which we needed.
- BBa_J04450 constructed to get the backbone.

Jul Wk 3

– The genetic circuit of the TDP began to be built.

Jul Wk 4

– Interlab experiments.

– Verified the activity of EpCAM.

Aug Wk 1

– The genetic circuit of the Kai began to be built.

Aug Wk 2

– BBa_K2623015 constructed.

- BBa_K2623009constructed.

– BBa_K2623016constructed.

- The genetic circuit of SAHS was completed and protein validation began.

Aug Wk 3

- BBa_K2623001 constructed.

- BBa_K2623022 and BBa_K2623024 constructed.

- BBa_I13401 constructed to get the GFP reporter.

Aug Wk 4

- BBa_K2623021 and BBa_K2623023 constructed.

- BBa_K2623026 constructed.

- BBa_K2623002 constructed.

- BBa_K2623003 constructed.

– Verified that SYL3C aptamer could combine with 10nt complementary chain successfully.

Sep Wk 1

- BBa_K2623014 constructed.

Sep Wk 2

- GFP on BBa_K2623021 and BBa_K2623023 successfully expressed.

- BBa_K2623004 constructed.

- BBa_K2623005 constructed.

- BBa_K2623006 constructed.

- First time successful purification of high-purity SAHS protein.

- Coating experiment on microfluidic chips succeeded.
- Started bacterial freeze-drying experiment.

Sep Wk 3

- BBa_E1010 constructed to get the mRFP reporter.

- BBa_K2623011 constructed.

- BBa_K2623007 constructed.

- Competition experiment succeeded.
- Started protein freeze-drying experiment.

– Verified the feasibility of “competition method” successfully.

Sep Wk 4

- BBa_K2623025 constructed.

- BBa_K2623008 constructed.

- Started try genetic recombination experiment.

Oct Wk 1

- BBa_K2623027 constructed.

- BBa_K2623013 constructed.

- NG2+5, NG2+6, NG4+5, NG4+6 constructed.

– Verified that As Cas12a could be activated.

Oct Wk 2

- BBa_K2623028, BBa_K2623029, BBa_K2623030, BBa_K2623031 successfully constructed and the fluorescence intensity of OMVs isolated from E.coli containing these four parts were detected by flow cytometer

– Verified the successful combination between SYL3C aptamer and EpCAM.

– Transferred molecular signal to fluorescence signal with DNase Alert.

Oct Wk 3

– Verified the transfer from protein signal to the fluorescent one successfully

Home
Model
Parts
Entrepreneurship
Attributions

Design
Hardware
Human Practices
Notebook
Judging

Results
Software
Collaborations
Members
After iGEM