Line 165: | Line 165: | ||
<p class="first" id="ca1">Gene Design</p> | <p class="first" id="ca1">Gene Design</p> | ||
<p class="second">National Taiwan University</p> | <p class="second">National Taiwan University</p> | ||
− | + | ||
+ | <div id="EX1" class="polaroid"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/4/49/T--CCU_Taiwan--JOE_experts_01.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
<p class="second">Professor K.S. Tung</p> | <p class="second">Professor K.S. Tung</p> | ||
<p class="description">Institute of Molecular and Cellular Biology<br>National Taiwan University</p> | <p class="description">Institute of Molecular and Cellular Biology<br>National Taiwan University</p> | ||
− | + | ||
+ | <div id="EX2" class="polaroid"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/d/d5/T--CCU_Taiwan--JOE_experts_02.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
<p class="description">He provided us constitutive type of promoter (ADH1 promoter), and auxotrophic marker (LEU2, URA3, TRP1).</p> | <p class="description">He provided us constitutive type of promoter (ADH1 promoter), and auxotrophic marker (LEU2, URA3, TRP1).</p> | ||
<p class="second">Professor C.T. Huang</p> | <p class="second">Professor C.T. Huang</p> | ||
Line 174: | Line 182: | ||
National Taiwan University | National Taiwan University | ||
</p> | </p> | ||
− | + | ||
+ | |||
+ | <div id="EX3" class="polaroid"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/0/0f/T--CCU_Taiwan--JOE_experts_03.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
<p class="description">He suggested using constitutive promoter without inducing by methanol. That is GAP promoter which is commonly used in <I>P. pastoris</I>. He also recommended that we could choose commercial pGAPZ A vector from Invitrogen.</p> | <p class="description">He suggested using constitutive promoter without inducing by methanol. That is GAP promoter which is commonly used in <I>P. pastoris</I>. He also recommended that we could choose commercial pGAPZ A vector from Invitrogen.</p> | ||
Line 180: | Line 193: | ||
<p class="first" id="ca2">Material Properties</p> | <p class="first" id="ca2">Material Properties</p> | ||
<p class="second">ITRI (Industrial Technology Research Institute)</p> | <p class="second">ITRI (Industrial Technology Research Institute)</p> | ||
− | + | <div id="EX4" class="polaroid"> | |
+ | <img src="https://static.igem.org/mediawiki/2018/2/26/T--CCU_Taiwan--JOE_EXPEARTS_NEW01.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
+ | |||
<p class="description">ITRI is a nonprofit R&D organization engaging in applied research and technical services. Founded in 1973, ITRI has played a vital role in transforming Taiwan's economy from a labor-intensive industry to a high-tech industry.</p> | <p class="description">ITRI is a nonprofit R&D organization engaging in applied research and technical services. Founded in 1973, ITRI has played a vital role in transforming Taiwan's economy from a labor-intensive industry to a high-tech industry.</p> | ||
<p class="second">Dr. Cheng-Han Hsieh</p> | <p class="second">Dr. Cheng-Han Hsieh</p> | ||
− | + | <div id="EX5" class="polaroid"> | |
+ | <img src="https://static.igem.org/mediawiki/2018/3/35/T--CCU_Taiwan--JOE_EXPEARTS_NEW02.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
<p class="description">(The fourth on the left, Dr. Hsieh)</p> | <p class="description">(The fourth on the left, Dr. Hsieh)</p> | ||
<p class="description">Research Fellow - Lignin Analytics<br> | <p class="description">Research Fellow - Lignin Analytics<br> | ||
Line 189: | Line 209: | ||
</p> | </p> | ||
<p class="second">Dr. Shu-Chuan Huang</p> | <p class="second">Dr. Shu-Chuan Huang</p> | ||
− | + | <div id="EX6" class="polaroid"> | |
+ | <img src="https://static.igem.org/mediawiki/2018/0/06/T--CCU_Taiwan--EXP_1.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
<p class="description">(The fourth on the left, Dr. Huang)</p> | <p class="description">(The fourth on the left, Dr. Huang)</p> | ||
<p class="description">Research Fellow – Material and Chemical Research<br> | <p class="description">Research Fellow – Material and Chemical Research<br> | ||
Line 196: | Line 219: | ||
<hr> | <hr> | ||
<p class="second">National Chung Hsing University</p> | <p class="second">National Chung Hsing University</p> | ||
− | + | ||
+ | |||
+ | <div id="EX7" class="polaroid"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/0/05/T--CCU_Taiwan--JOE_experts_07.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
<p class="second">Prof. Yuan-Shing Perng</p> | <p class="second">Prof. Yuan-Shing Perng</p> | ||
<p class="description">Department of Forestry<br> | <p class="description">Department of Forestry<br> | ||
National Chung Hsing University | National Chung Hsing University | ||
</p> | </p> | ||
− | + | ||
+ | |||
+ | <div id="EX8" class="polaroid"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/c/ce/T--CCU_Taiwan--JOE_EXPEARTS_NEW03.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
+ | |||
<p class="description">He helped us test whether the paper laminated with LIGGREEN can be separated into paper pulping and LIGGREEN. In the interview with professor Perng, he confirmed that a small amount of lignin mixed into the ordinary pulp will not affect the paper remaking process, maybe LIGGREEN can be separated, too. He taught us to operate the pulper, after 200 seconds of pulping and beating, LIGGREEN and the paper were indeed separated. This proved that LIGGREEN would not harm in the pulp recycling system. | <p class="description">He helped us test whether the paper laminated with LIGGREEN can be separated into paper pulping and LIGGREEN. In the interview with professor Perng, he confirmed that a small amount of lignin mixed into the ordinary pulp will not affect the paper remaking process, maybe LIGGREEN can be separated, too. He taught us to operate the pulper, after 200 seconds of pulping and beating, LIGGREEN and the paper were indeed separated. This proved that LIGGREEN would not harm in the pulp recycling system. | ||
</p> | </p> | ||
Line 208: | Line 242: | ||
National Chung Hsing University | National Chung Hsing University | ||
</p> | </p> | ||
− | + | ||
+ | <div id="EX9" class="polaroid"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/c/c4/T--CCU_Taiwan--JOE_experts_09.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
<p class="description">He told us that in the reaction forming LIGGREEN from monolignols bonding to oligomers, there are some bonding nodes would act. He suggested that the oligomers we produced were preferably two-dimensional chain systems, not three-dimensional, to have thermoplastic properties. | <p class="description">He told us that in the reaction forming LIGGREEN from monolignols bonding to oligomers, there are some bonding nodes would act. He suggested that the oligomers we produced were preferably two-dimensional chain systems, not three-dimensional, to have thermoplastic properties. | ||
</p> | </p> | ||
<hr> | <hr> | ||
<p class="second">Recycling Fund Management Board</p> | <p class="second">Recycling Fund Management Board</p> | ||
− | + | ||
+ | <div id="EX10" class="polaroid"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/0/0b/T--CCU_Taiwan--JOE_EXPEARTS_NEW04.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
<p class="second">Meng-Erh Wu</p> | <p class="second">Meng-Erh Wu</p> | ||
<p class="description">Deputy executive secretary of Recycling Fund Management Board | <p class="description">Deputy executive secretary of Recycling Fund Management Board | ||
Line 223: | Line 265: | ||
<p class="first" id="ca3">Production Line</p> | <p class="first" id="ca3">Production Line</p> | ||
<p class="second">Bobsen Paper Corporation</p> | <p class="second">Bobsen Paper Corporation</p> | ||
− | + | ||
+ | <div id="EX11" class="polaroid"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/5/58/T--CCU_Taiwan--JOE_experts_11.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
+ | |||
<p class="description">Bobsen Paper Corporation is responsible for producing coated paper and assisting downstream manufacturers in creating creases on paper. After our fundraising session, Bobsen agreed with us to arrange a corporate visit. Let us visit the factory and equipment of the paper laminating and answer our doubts about the production line. | <p class="description">Bobsen Paper Corporation is responsible for producing coated paper and assisting downstream manufacturers in creating creases on paper. After our fundraising session, Bobsen agreed with us to arrange a corporate visit. Let us visit the factory and equipment of the paper laminating and answer our doubts about the production line. | ||
</p> | </p> | ||
Line 232: | Line 279: | ||
National Chung Cheng University | National Chung Cheng University | ||
</p> | </p> | ||
− | + | ||
+ | |||
+ | <div id="EX12" class="polaroid"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/1/14/T--CCU_Taiwan--JOE_experts_12.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
<p class="description">He told us the truth of recycling in Taiwan, he also told us our product would not influence the paper recycled system because the amount of our LIGGREEN is very low and we do not need to set a recycled spot of LIGGREEN cups. | <p class="description">He told us the truth of recycling in Taiwan, he also told us our product would not influence the paper recycled system because the amount of our LIGGREEN is very low and we do not need to set a recycled spot of LIGGREEN cups. | ||
</p> | </p> | ||
<hr> | <hr> | ||
<p class="second">Lian-Tai</p> | <p class="second">Lian-Tai</p> | ||
− | + | ||
+ | |||
+ | <div id="EX13" class="polaroid"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/e/e4/T--CCU_Taiwan--JOE_experts_13.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
<p class="description">Lian-Tai is the only manufacturer in Taiwan that can separate paper from plastic lining. He helped us understand Taiwan's current recycling environment and recycling issues, you can refer to more details in iHP. | <p class="description">Lian-Tai is the only manufacturer in Taiwan that can separate paper from plastic lining. He helped us understand Taiwan's current recycling environment and recycling issues, you can refer to more details in iHP. | ||
</p> | </p> | ||
<p class="second">Chung Hwa Pulp Corporation</p> | <p class="second">Chung Hwa Pulp Corporation</p> | ||
− | + | <div id="EX14" class="polaroid"> | |
+ | <img src="https://static.igem.org/mediawiki/2018/d/d7/T--CCU_Taiwan--JOE_experts_14.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
<p class="description">Chung Hwa Pulp Corporation provided us simple paper pulping and making process, they also answered the raw materials of paper. | <p class="description">Chung Hwa Pulp Corporation provided us simple paper pulping and making process, they also answered the raw materials of paper. | ||
</p> | </p> | ||
Line 249: | Line 311: | ||
<p class="description">CEO of THEFREEN BURGER | <p class="description">CEO of THEFREEN BURGER | ||
</p> | </p> | ||
− | + | ||
− | + | <div id="EX15" class="polaroid"> | |
+ | <img src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--JOE_EXPEARTS_NEW05.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
+ | <div id="EX16" class="polaroid"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/2/25/T--CCU_Taiwan--JOE_EXPEARTS_NEW06.jpg" style="width:100%"> | ||
+ | </div> | ||
+ | |||
+ | |||
<p class="description">He made us think about how to conduct market analysis to better develop LIGGREEN. He also reminded us to investigate the status of similar industries in the existing market, in order to better construct the business model and strengthen the link between LOGGREEN and society. | <p class="description">He made us think about how to conduct market analysis to better develop LIGGREEN. He also reminded us to investigate the status of similar industries in the existing market, in order to better construct the business model and strengthen the link between LOGGREEN and society. | ||
</p> | </p> |
Revision as of 01:52, 18 October 2018
ENGAGING EXPERTS
Gene Design
National Taiwan University
Professor K.S. Tung
Institute of Molecular and Cellular Biology
National Taiwan University
He provided us constitutive type of promoter (ADH1 promoter), and auxotrophic marker (LEU2, URA3, TRP1).
Professor C.T. Huang
Department of Biochemical Science and Technology
National Taiwan University
He suggested using constitutive promoter without inducing by methanol. That is GAP promoter which is commonly used in P. pastoris. He also recommended that we could choose commercial pGAPZ A vector from Invitrogen.
Material Properties
ITRI (Industrial Technology Research Institute)
ITRI is a nonprofit R&D organization engaging in applied research and technical services. Founded in 1973, ITRI has played a vital role in transforming Taiwan's economy from a labor-intensive industry to a high-tech industry.
Dr. Cheng-Han Hsieh
(The fourth on the left, Dr. Hsieh)
Research Fellow - Lignin Analytics
He recommended that we have to control the LIGGREEN in micromolecular to make it have the melting point. He also gave us some directions for decomposing lignin-like material. The Bacillus subtilis may be our good choice.
Dr. Shu-Chuan Huang
(The fourth on the left, Dr. Huang)
Research Fellow – Material and Chemical Research
She recommended that we should refer to the ISO 9000, ASTM D6400, and so on. Then, she recommended that we should cut the material into small pieces, carry out industrial composting, and detect the decomposition ratio.
National Chung Hsing University
Prof. Yuan-Shing Perng
Department of Forestry
National Chung Hsing University
He helped us test whether the paper laminated with LIGGREEN can be separated into paper pulping and LIGGREEN. In the interview with professor Perng, he confirmed that a small amount of lignin mixed into the ordinary pulp will not affect the paper remaking process, maybe LIGGREEN can be separated, too. He taught us to operate the pulper, after 200 seconds of pulping and beating, LIGGREEN and the paper were indeed separated. This proved that LIGGREEN would not harm in the pulp recycling system.
Prof. Wen-Jau Lee
Department of Forestry
National Chung Hsing University
He told us that in the reaction forming LIGGREEN from monolignols bonding to oligomers, there are some bonding nodes would act. He suggested that the oligomers we produced were preferably two-dimensional chain systems, not three-dimensional, to have thermoplastic properties.
Recycling Fund Management Board
Meng-Erh Wu
Deputy executive secretary of Recycling Fund Management Board
He recommended that our products may need to consider the shelf life, because some paper containers such as milk carton are packaging beverage for a long time and the lining of them cannot be leach. And he mentioned that the government used the method of Administrative guidance and Subsidy incentive to try to improve the recovery yield.
However, some misunderstandings in the process led to false positives in the news, causing the public to throw paper containers into general garbage disposal.
Production Line
Bobsen Paper Corporation
Bobsen Paper Corporation is responsible for producing coated paper and assisting downstream manufacturers in creating creases on paper. After our fundraising session, Bobsen agreed with us to arrange a corporate visit. Let us visit the factory and equipment of the paper laminating and answer our doubts about the production line.
Recycling Program
Prof. Ping-Mu Shu
Department of Earth and Environmental Science
National Chung Cheng University
He told us the truth of recycling in Taiwan, he also told us our product would not influence the paper recycled system because the amount of our LIGGREEN is very low and we do not need to set a recycled spot of LIGGREEN cups.
Lian-Tai
Lian-Tai is the only manufacturer in Taiwan that can separate paper from plastic lining. He helped us understand Taiwan's current recycling environment and recycling issues, you can refer to more details in iHP.
Chung Hwa Pulp Corporation
Chung Hwa Pulp Corporation provided us simple paper pulping and making process, they also answered the raw materials of paper.
Enterpreneumship
Yong-Zhong Chen
CEO of THEFREEN BURGER
He made us think about how to conduct market analysis to better develop LIGGREEN. He also reminded us to investigate the status of similar industries in the existing market, in order to better construct the business model and strengthen the link between LOGGREEN and society.