Difference between revisions of "Team:NAU-CHINA/Safety"

 
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<li>safety</li>
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        <p class="top-title">SAFETY</p>
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<li>Safe Project Design</li>
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<li> We confirmed that only non-pathogenic strains will be used and no animal or plant infestation experiments will be conducted. E. coli DH5a, jurkat cell line and 293t cell line have been widely used in scientific research and is not harmful to the environment itself, and mammalian cell lines are difficult to survive outside the laboratory. The Lentivirus vector has deleted the toxic gene and it is a pseudotyped virus. Therefore, it has no toxic effect and it is not easy to infect the host outside the laboratory. In addition, we will not insert any toxic or harmful genes into our lab.<br>
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Because our project is foundational, we don't have a specific real-word application. We won't use our system in any fields where can likely be in danger.If we eventually use our project for some fields such as cancer field, we construct the cellular pathways we envisioned in T-lymphocytes. Then if an error occurs in the process of genetic modification, leading to the death of T cells or unexpected toxicity, it will cause great harm to cancer patients. Therefore, our projects do not pose any risks in the real world. <br>
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Morally speaking, these risks are small or non-existent given that we do not intend to use animals or introduce anything into the environment. <br>
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Since we are conducting basic research, all our experiments will be carried out in the laboratory. Most of the experiments are very basic experiments such as molecular cloning, cell culture, and fluorescence processing, and the risks to the human body and the environment are very low. Lentiviral vectors and infected cells are relatively risky to the human body. But given that this technology has been widely used, and many iGEM teams have used this method, as long as we strictly abide by the experimental specifications, we can minimize the risk.<br>
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The principle investigators Jiandong Jiang and Jingyu Lang are responsible for the safety and security of biology labs. Both of them have reviewed our experimental plan and given us some advice on the safety and security of these experiments.They are familiar with some of the biosafety and biosecurity risks that may be encountered during the use of E. coli, mammalian cells, and lentiviral vectors.<br>
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<li>Safe Lab Work</li>
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<li>We read and signed the laboratory safety responsibility statement, which includes:</li>
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        <li>Wear lab coats when entering the laboratory, gloves and other armor for experimentation.</li>
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                <li>It is strictly forbidden to smoke, eat or drink any food in the laboratory or use laboratory equipment (such as electric stoves, microwave ovens, etc.) to cook the food.</li>
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                <li>When using the equipment, the experiment should be carried out in strict accordance with the operating procedures, and the unfamiliar instruments should be familiar and reused.</li>
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                <li>Strictly categorize experimental waste and raw domestic waste.</li>
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                <li>Chemical waste generated by the experiment should be placed at a special storage point and must not be dumped or placed indiscriminately.</li>
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                <li>When a safety accident occurs, the power should be cut off first, scientifically processed, etc.<br>
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Click to view the full agreement:<br>
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(Laboratory Safety Responsibility Document doc.)<br>
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We received two hours of laboratory safety training at the director of the Experimental Center Office of the College of Life Sciences at Nanjing Agricultural University. All training courses are documented. The entire lab team previously worked in the same biosafety level laboratory, and the team members were experienced and familiar with the job requirements and environment. Every member entering the laboratory must pass the biosafety and biosafety exams and be familiar with all safety equipment locations and safety planning routes for a particular laboratory space. In addition, we have strict regulations on the treatment of experimental waste. All contaminated waste must be sterilized before it can be disposed of. For discarded drugs, they are handled uniformly by the college.<br>
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            <p>This year, NAU-CHINA is trying to build a genetic circuit "MOSFETs" in animal T cells which is "Monitoring and Operating System Founded on Engineered T cells". Here we are going to share our experience in safety control.</p>
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            <h1>Safe Project Design</h1>
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                <h2>Choosing a non-pathogenic chassis</h2>
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                <p>In our project, non-pathogenic cell line of jurkat cell was the chassis used to transfection and protein expression, what’s more, non-pathogenic strains of E. coli were the only chassis used to perform molecular cloning.</p>
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                <h2>Choosing parts that will be environmental friendly</h2>
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                <p>Harmful parts are not allowed in our project. The parts in our project are mostly commonly used Notch protein, recombinases and promoters, so that none of our parts would raise any safety issues according to current professional knowledge.
  
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            <h1>Safe Lab Work</h1>
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                <h2>Biosafety and biosecurity exam</h2>
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                <p>We stipulate that only those who have passed the online security exam can enter the laboratory. The safety test questions are determined by the School Experimental Safety Committee and cover the daily safety and biosafety of the laboratory.</p>
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                <h2>Daily lab work</h2>
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                <p>According to the requirements of iGEM policy, in our daily laboratory work we never performed any dangerous experiments or faced any unusual safety issues. We strictly followed some basic regulations as follows:</p>
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                <p>1. Participants need to understand the experiment completely.</p>
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                <p>2. Wear rubber gloves during all experimenters.</p>
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                <p>3. Steps with any probability of contamination should be performed in a biosafety cabinet.</p>
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                <p>4. Fire, electric heaters, and microwave ovens should never be left unattended.</p>
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                <p>5. All liquid and solid waste containing living organisms must be autoclaved.</p>
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                <p>6. The last person to leave the lab should make sure that water, electricity, gas, and air conditioners have been turned off, and doors and windows have been locked before leaving.</p>
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            <h1>Ethical Risks</h1>           
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              Although we have some applied outlook for our project, we currently do not conduct validation experiments in animals or the environment.
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            <p>If you want to know more about our safety, you can visit our Safety Form.</p>
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Latest revision as of 01:52, 18 October 2018

Template:2018_NAU-CHINA

header
Safety

SAFETY

This year, NAU-CHINA is trying to build a genetic circuit "MOSFETs" in animal T cells which is "Monitoring and Operating System Founded on Engineered T cells". Here we are going to share our experience in safety control.

Safe Project Design

Choosing a non-pathogenic chassis

In our project, non-pathogenic cell line of jurkat cell was the chassis used to transfection and protein expression, what’s more, non-pathogenic strains of E. coli were the only chassis used to perform molecular cloning.

Choosing parts that will be environmental friendly

Harmful parts are not allowed in our project. The parts in our project are mostly commonly used Notch protein, recombinases and promoters, so that none of our parts would raise any safety issues according to current professional knowledge.

Safe Lab Work

Biosafety and biosecurity exam

We stipulate that only those who have passed the online security exam can enter the laboratory. The safety test questions are determined by the School Experimental Safety Committee and cover the daily safety and biosafety of the laboratory.

Daily lab work

According to the requirements of iGEM policy, in our daily laboratory work we never performed any dangerous experiments or faced any unusual safety issues. We strictly followed some basic regulations as follows:

1. Participants need to understand the experiment completely.

2. Wear rubber gloves during all experimenters.

3. Steps with any probability of contamination should be performed in a biosafety cabinet.

4. Fire, electric heaters, and microwave ovens should never be left unattended.

5. All liquid and solid waste containing living organisms must be autoclaved.

6. The last person to leave the lab should make sure that water, electricity, gas, and air conditioners have been turned off, and doors and windows have been locked before leaving.

Ethical Risks

Although we have some applied outlook for our project, we currently do not conduct validation experiments in animals or the environment.

If you want to know more about our safety, you can visit our Safety Form.

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