Difference between revisions of "Team:NAU-CHINA/Safety"

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    <title>Safety</title>
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        <p class="top-title">SAFETY</p>
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<h1> Safety </h1>
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<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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        <div class="textblock">
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            <p>This year, NAU-CHINA is trying to build a genetic circuit "MOSFETs" in animal T cells which is "Monitoring and Operating System Founded on Engineered T cells". Here we are going to share our experience in safety control.</p>
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            <h1>Safe Project Design</h1>
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            <div class="section">
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                <h2>Choosing a non-pathogenic chassis</h2>
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                <p>In our project, non-pathogenic cell line of jurkat cell was the chassis used to transfection and protein expression, what’s more, non-pathogenic strains of E. coli were the only chassis used to perform molecular cloning.</p>
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            </div>
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            <div class="section">
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                <h2>Choosing parts that will be environmental friendly</h2>
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                <p>Harmful parts are not allowed in our project. The parts in our project are mostly commonly used Notch protein, recombinases and promoters, so that none of our parts would raise any safety issues according to current professional knowledge.
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h3>Safe Project Design</h3>
 
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
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<ul>
 
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<li>Choosing a non-pathogenic chassis</li>
 
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<li>Choosing parts that will not harm humans / animals / plants</li>
 
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
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<li>Including an "induced lethality" or "kill-switch" device</li>
 
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<h3>Safe Lab Work</h3>
 
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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            <h1>Safe Lab Work</h1>
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            <div class="section">
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                <h2>Biosafety and biosecurity exam</h2>
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                <p>We stipulate that only those who have passed the online security exam can enter the laboratory. The safety test questions are determined by the School Experimental Safety Committee and cover the daily safety and biosafety of the laboratory.</p>
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            </div>
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            <div class="section">
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                <h2>Daily lab work</h2>
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                <p>According to the requirements of iGEM policy, in our daily laboratory work we never performed any dangerous experiments or faced any unusual safety issues. We strictly followed some basic regulations as follows:</p>
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                <p>1. Participants need to understand the experiment completely.</p>
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                <p>2. Wear rubber gloves during all experimenters.</p>
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                <p>3. Steps with any probability of contamination should be performed in a biosafety cabinet.</p>
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                <p>4. Fire, electric heaters, and microwave ovens should never be left unattended.</p>
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                <p>5. All liquid and solid waste containing living organisms must be autoclaved.</p>
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                <p>6. The last person to leave the lab should make sure that water, electricity, gas, and air conditioners have been turned off, and doors and windows have been locked before leaving.</p>
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<h3>Safe Shipment</h3>
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            <h1>Ethical Risks</h1>           
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            <p>
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              Although we have some applied outlook for our project, we currently do not conduct validation experiments in animals or the environment.
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            </p>
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            <p>If you want to know more about our safety, you can visit our Safety Form.</p>
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        </div>
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    </div>
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
 
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{{NAU-CHINA/footer}}

Latest revision as of 01:52, 18 October 2018

Template:2018_NAU-CHINA

header
Safety

SAFETY

This year, NAU-CHINA is trying to build a genetic circuit "MOSFETs" in animal T cells which is "Monitoring and Operating System Founded on Engineered T cells". Here we are going to share our experience in safety control.

Safe Project Design

Choosing a non-pathogenic chassis

In our project, non-pathogenic cell line of jurkat cell was the chassis used to transfection and protein expression, what’s more, non-pathogenic strains of E. coli were the only chassis used to perform molecular cloning.

Choosing parts that will be environmental friendly

Harmful parts are not allowed in our project. The parts in our project are mostly commonly used Notch protein, recombinases and promoters, so that none of our parts would raise any safety issues according to current professional knowledge.

Safe Lab Work

Biosafety and biosecurity exam

We stipulate that only those who have passed the online security exam can enter the laboratory. The safety test questions are determined by the School Experimental Safety Committee and cover the daily safety and biosafety of the laboratory.

Daily lab work

According to the requirements of iGEM policy, in our daily laboratory work we never performed any dangerous experiments or faced any unusual safety issues. We strictly followed some basic regulations as follows:

1. Participants need to understand the experiment completely.

2. Wear rubber gloves during all experimenters.

3. Steps with any probability of contamination should be performed in a biosafety cabinet.

4. Fire, electric heaters, and microwave ovens should never be left unattended.

5. All liquid and solid waste containing living organisms must be autoclaved.

6. The last person to leave the lab should make sure that water, electricity, gas, and air conditioners have been turned off, and doors and windows have been locked before leaving.

Ethical Risks

Although we have some applied outlook for our project, we currently do not conduct validation experiments in animals or the environment.

If you want to know more about our safety, you can visit our Safety Form.

footer