Difference between revisions of "Team:NAU-CHINA/Parts"

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{{NAU-CHINA/ResetCSS}}
 
 
{{NAU-CHINA}}
 
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{{NAU-CHINA/header}}
 
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<html>
 
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<head>
 
<head>
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<style>
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<meta charset="utf-8" />
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p {
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    <title>Parts</title>
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    <script>
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        $(document).ready(function () {
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            $('#banner img').attr('src', 'https://static.igem.org/mediawiki/2018/b/b5/T--NAU-China--bannerparts.jpg')
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        });
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    <style>
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          border-color:black;
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      .smaller-image {
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          height: 280px;
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<body>
 
<body>
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<hr/>
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    <div class="topLine">
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<p>
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        <p class="top-title">Results</p>
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        <p class="sec-title">Parts</p>
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anti-GFP-mnotch-TEV-NLS-pSB1C3<br>
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    </div>
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The anti-GFP-mnotch-tetR-VP64 of Fudan University was transformed into anti-GFP-mnotch-TEV, and the flag tag and NLS were added at the right end. Make it our cell surface receptor after transformation.<br>
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    <a href="https://2018.igem.org/Team:NAU-CHINA/Demonstrate">
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插图，细胞图、western bolt结果<br>
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        <img id="icon1" class="guide-icon" src="https://static.igem.org/mediawiki/2018/6/66/T--NAU-China--demo.png" />
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<br>
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    </a>
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Bxb1 integrase-pSB1C3<br>
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Full length sequence of humanized Bxb1 integrase,<br>
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Bxb1-reporter-pSB1C3<br>
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Inserting a reporter gene in the middle of the Bxb1 integrase recombination site<br>
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Bxb1-site-pSB1C3<br>
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Bxb1 integrase recombination site sequence<br>
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PhiC31 integrase-pSB1C3
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Full-length sequence of humanized PhiC31 integrase
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PhiC31-reporter-pSB1C3
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Inserting a reporter gene in the middle of the PhiC31 integrase recombination site
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PhiC31-site-pSB1C3
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PhiC31 integrase recombination site sequence
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<br>
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TP901 integrase-pSB1C3<br>
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<a href="https://2018.igem.org/Team:NAU-CHINA/Basic_Part">
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Humanized TP901 integrase full length sequence<br>
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  <p class="smaller-image thick-border"  rows="10" cols="30">
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TP901 -reporter-pSB1C3<br>
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<b>Our Best Basic Part</b><br><br>
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Inserting a reporter gene into the TP901 integrase recombination site<br>
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Here we present the best characterized recombinase - Bxb1 (BBa_K2557001) as our best basic part.
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TP901-site-pSB1C3<br>
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</p>  
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TP901 integrase recombination site sequence<br>
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</a>
  
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<br>
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<a href="https://2018.igem.org/Team:NAU-CHINA/Composite_Part">
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TetO-miniCMV-Bxb1-pSB1C3<br>
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  <p class="smaller-image thick-border"  rows="10" cols="30">
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The TetO sequence, SV40 NLS, flag tag, miniCMV promoter, Bxb1 integrase are sequentially linked and involved in intracellular signal processing for detecting the strength of the promoter and the effect of different recombinases on the system. <br>
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<b>Our Best Composite Part</b><br><br>
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插细胞荧光图<br>
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We choose TetO-miniCMV promoter-Bxb1(BBa_K2557010) as our best composite part.
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TetO-EF1a-Bxb1-pSB1C3<br>
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</p>  
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The TetO sequence, SV40 NLS, flag tag, EF1a promoter, and Bxb1 integrase are ligated in sequence, and participate in intracellular signal processing to detect the effect of promoter strength and different recombinases on the system.<br>
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</a>
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插细胞荧光图<br>
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TetO-miniCMV-Tp901-pSB1C3<br>
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The TetO sequence, SV40 NLS, flag tag, miniCMV promoter, and Tp901 integrase are ligated in sequence to participate in intracellular signal processing for detecting the strength of the promoter and the effects of different recombinases on the system.<br>
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插细胞荧光图<br>
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TetO-EF1a-Tp901-pSB1C3<br>
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The TetO sequence, SV40 NLS, flag tag, EF1a promoter, and Tp901 integrase are ligated in sequence, and participate in intracellular signal processing to detect the effect of promoter strength and different recombinases on the system.<br>
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插细胞荧光图<br>
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<br>
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<a href="https://2018.igem.org/Team:NAU-CHINA/Improve">
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TetO-EF1a-PhiC31-pSB1C3<br>
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  <p class="smaller-image thick-border"  rows="10" cols="30">
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The TetO sequence, SV40 NLS, flag tag, EF1a promoter, and PhiC31 integrase are ligated in sequence, and participate in intracellular signal processing to detect the effect of promoter strength and different recombinases on the system.<br>
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<b>Our Improved Part</b><br><br>
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插细胞荧光图<br>
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Bxb1 (BBa_K2557001) is improved from BBa_K2243012.
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</p>  
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</a>
  
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<br>
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<a href="https://2018.igem.org/Team:NAU-CHINA/Part_Collection">
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EF1a-EGFP-pSB1C3<br>
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  <p class="smaller-image thick-border"  rows="10" cols="30">
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The EF1a promoter and EGFP coding sequences were ligated in sequence to characterize promoter strength.<br>
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<b>Our Part Collection</b><br><br>
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插细胞荧光图<br>
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We present the switch consisting of Bxb1 and Bxb1-RDF as the Our Part Collection.
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</p>  
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</a>
  
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<br>
 
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miniCMV-EGFP-pSB1C3<br>
 
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The miniCMV promoter and the EGFP coding sequence were ligated in sequence for characterizing the promoter strength.<br>
 
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插细胞荧光图<br>
 
  
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<br>
 
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Ubc-EGFP-pSB1C3<br>
 
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The Ubc promoter and EGFP coding sequences were ligated in sequence for characterizing promoter strength.<br>
 
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插细胞荧光图<br>
 
  
  
  
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</p>
 
 
</body>
 
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{{NAU-CHINA/footer}}

Latest revision as of 01:55, 18 October 2018

Template:2018_NAU-CHINA

header
Parts

Results

Parts

Our Best Basic Part

Here we present the best characterized recombinase - Bxb1 (BBa_K2557001) as our best basic part.

Our Best Composite Part

We choose TetO-miniCMV promoter-Bxb1(BBa_K2557010) as our best composite part.

Our Improved Part

Bxb1 (BBa_K2557001) is improved from BBa_K2243012.

Our Part Collection

We present the switch consisting of Bxb1 and Bxb1-RDF as the Our Part Collection.

footer