Difference between revisions of "Team:TU-Eindhoven/Basic Part"

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            <div id="introDiv" class="col-xs-12 introduction">
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                <h1 style="text-align: center;">Parts overview</h1>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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                <div class="toc-wrapper">
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                    <li>
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                        <ul id="tableOfContent">
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                            <p>The best that most of us can hope to achieve in physics is simply to misunderstand at a deeper level.</p>
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                        </ul>
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                        <a class="backToTop" onclick="topFunction()">▲</a>
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<h1>Basic Parts</h1>
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                    <p>To realize our designed parts, we gratefully made use of the IDT Synthesis offer,
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<p>
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                        allowing easy and fast access to the desired DNA constructs. All parts are codon
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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                        optimized for expression in E. coli, the workhorse of our project. For all our composite parts,
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</p>
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                        the comprising basic parts were registered separately on the Registry, to allow future iGEM teams to
 +
                        use our constructs in new assemblies. All parts we shipped to the registry are sequence confirmed.
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                        We nominated <a href="http://parts.igem.org/Part:BBa_K2812004">BBa_K2812004</a>, lysostaphin-thrombin linker-HlyA-His tag, as Best New Basic Part as we think
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                        that the realisation of secretable lysostaphin opens up a whole new area of projects and applications,
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                        since the issues and limitations associated with cell lysis are avoided. The T7-Lysostaphin-thrombin
 +
                        linker-HlyA-His part, <a href="http://parts.igem.org/Part:BBa_K2812005">BBa_K2812005</a>, has been nominated for Best New Composite Part, as it allows direct
 +
                        secretion of lysostaphin from the iGEM shipping backbone. This enables future teams to have a head start in
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                        their project.</p>
  
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
 
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<div class="highlight decoration_background">
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                    <table class="parts-table">
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<h3>Note</h3>
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                      <caption>All Parts</caption>
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
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                      <tr>
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</div>
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                        <th>Fave Part</th>
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</div>
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                        <th>Name</th>
 +
                        <th>Type</th>
 +
                        <th>Description</th>
 +
                        <th>Designers</th>
 +
                        <th>Length</th>
 +
                      </tr>
 +
                      <tr class="odd-tr">
 +
                        <td><span style="color:red">❤</span></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812000">BBa_K2812000</a></td>
 +
                        <td>Basic</td>
 +
                        <td>Carbohydrate-binding domain from MpIBP</td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
 +
                        <td>576</td>
 +
                      </tr>
 +
                      <tr class="even-tr">
 +
                        <td></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812001">BBa_K2812001</a></td>
 +
                        <td>Basic</td>
 +
                        <td>Coding sequence for trunctated Lysostaphin</td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
 +
                        <td>738</td>
 +
                      </tr>
 +
                      <tr class="odd-tr">
 +
                        <td></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812002">BBa_K2812002</a></td>
 +
                        <td>Basic</td>
 +
                        <td>pBAD-ara promoter - Arabinose inducible regulatory promoter </td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls</td>
 +
                        <td>71</td>
 +
                      </tr>
 +
                      <tr  class="even-tr">
 +
                        <td></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812003">BBa_K2812003</a></td>
 +
                        <td>Composite</td>
 +
                        <td>Coding sequence for trunctated Lysostaphin regulated by T7-promoter</td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls</td>
 +
                        <td>776</td>
 +
                      </tr>
 +
                      <tr class="odd-tr">
 +
                        <td><span style="color:red">❤</span></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812004">BBa_K2812004</a></td>
 +
                        <td>Basic</td>
 +
                        <td>Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA</td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
 +
                        <td>1458</td>
 +
                      </tr>
 +
                      <tr  class="even-tr">
 +
                        <td><span style="color:red">❤</span></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812005">BBa_K2812005</a></td>
 +
                        <td>Composite</td>
 +
                        <td>Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter</td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
 +
                        <td>1496</td>
 +
                      </tr>
 +
                      <tr class="odd-tr">
 +
                        <td></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812006">BBa_K2812006</a></td>
 +
                        <td>Basic</td>
 +
                        <td>Coding sequence for Pyocin S5 with HlyA and His6-tag </td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
 +
                        <td>2217</td>
 +
                      </tr>
 +
                      <tr  class="even-tr">
 +
                        <td></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812007">BBa_K2812007</a></td>
 +
                        <td>Composite</td>
 +
                        <td>Coding sequence for Pyosin S5 with HlyA and His6-tag regulated by AraC promoter</td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
 +
                        <td>2288</td>
 +
                      </tr>
 +
                    </table>
  
 +
                    <table class="parts-table">
 +
                      <caption>Basic Parts</caption>
 +
                      <tr>
 +
                        <th>Fave Part</th>
 +
                        <th>Name</th>
 +
                        <th>Type</th>
 +
                        <th>Description</th>
 +
                        <th>Designers</th>
 +
                        <th>Length</th>
 +
                      </tr>
 +
                      <tr class="odd-tr">
 +
                        <td><span style="color:red">❤</span></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812004">BBa_K2812004</a></td>
 +
                        <td>Basic</td>
 +
                        <td>Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA</td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
 +
                        <td>1458</td>
 +
                      </tr>
 +
                      <tr class="even-tr">
 +
                        <td><span style="color:red">❤</span></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812000">BBa_K2812000</a></td>
 +
                        <td>Basic</td>
 +
                        <td>Carbohydrate-binding domain from MpIBP</td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
 +
                        <td>576</td>
 +
                      </tr>
 +
                      <tr class="odd-tr">
 +
                        <td></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812001">BBa_K2812001</a></td>
 +
                        <td>Basic</td>
 +
                        <td>Coding sequence for trunctated Lysostaphin</td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
 +
                        <td>738</td>
 +
                      </tr>
 +
                      <tr class="even-tr">
 +
                        <td></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812002">BBa_K2812002</a></td>
 +
                        <td>Basic</td>
 +
                        <td>pBAD-ara promoter - Arabinose inducible regulatory promoter </td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls</td>
 +
                        <td>71</td>
 +
                      </tr>
  
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<div class="column full_size">
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                      <tr class="odd-tr">
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<h3>Best Basic Part Special Prize</h3>
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                        <td></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812006">BBa_K2812006</a></td>
 +
                        <td>Basic</td>
 +
                        <td>Coding sequence for Pyocin S5 with HlyA and His6-tag </td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
 +
                        <td>2217</td>
 +
                      </tr>
 +
                    </table>
  
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<p> To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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                    <table class="parts-table">
 +
                      <caption>All Parts</caption>
 +
                      <tr>
 +
                        <th>Fave Part</th>
 +
                        <th>Name</th>
 +
                        <th>Type</th>
 +
                        <th>Description</th>
 +
                        <th>Designers</th>
 +
                        <th>Length</th>
 +
                      </tr>
  
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<br><br>
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                      <tr  class="even-tr">
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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                        <td><span style="color:red">❤</span></td>
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</div>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812005">BBa_K2812005</a></td>
 +
                        <td>Composite</td>
 +
                        <td>Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter</td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
 +
                        <td>1496</td>
 +
                      </tr>
  
 +
                      <tr  class="odd-tr">
 +
                        <td></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812003">BBa_K2812003</a></td>
 +
                        <td>Composite</td>
 +
                        <td>Coding sequence for trunctated Lysostaphin regulated by T7-promoter</td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls</td>
 +
                        <td>776</td>
 +
                      </tr>
  
 +
                      <tr  class="even-tr">
 +
                        <td></td>
 +
                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812007">BBa_K2812007</a></td>
 +
                        <td>Composite</td>
 +
                        <td>Coding sequence for Pyosin S5 with HlyA and His6-tag regulated by AraC promoter</td>
 +
                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
 +
                        <td>2288</td>
 +
                      </tr>
 +
                    </table>
  
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Revision as of 02:35, 18 October 2018

Parts overview

    • The best that most of us can hope to achieve in physics is simply to misunderstand at a deeper level.

    ▲

    • To realize our designed parts, we gratefully made use of the IDT Synthesis offer, allowing easy and fast access to the desired DNA constructs. All parts are codon optimized for expression in E. coli, the workhorse of our project. For all our composite parts, the comprising basic parts were registered separately on the Registry, to allow future iGEM teams to use our constructs in new assemblies. All parts we shipped to the registry are sequence confirmed. We nominated BBa_K2812004, lysostaphin-thrombin linker-HlyA-His tag, as Best New Basic Part as we think that the realisation of secretable lysostaphin opens up a whole new area of projects and applications, since the issues and limitations associated with cell lysis are avoided. The T7-Lysostaphin-thrombin linker-HlyA-His part, BBa_K2812005, has been nominated for Best New Composite Part, as it allows direct secretion of lysostaphin from the iGEM shipping backbone. This enables future teams to have a head start in their project.

    All Parts
    Fave Part Name Type Description Designers Length
    ❤ BBa_K2812000 Basic Carbohydrate-binding domain from MpIBP Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 576
    BBa_K2812001 Basic Coding sequence for trunctated Lysostaphin Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 738
    BBa_K2812002 Basic pBAD-ara promoter - Arabinose inducible regulatory promoter Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls 71
    BBa_K2812003 Composite Coding sequence for trunctated Lysostaphin regulated by T7-promoter Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls 776
    ❤ BBa_K2812004 Basic Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 1458
    ❤ BBa_K2812005 Composite Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 1496
    BBa_K2812006 Basic Coding sequence for Pyocin S5 with HlyA and His6-tag Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 2217
    BBa_K2812007 Composite Coding sequence for Pyosin S5 with HlyA and His6-tag regulated by AraC promoter Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 2288
    Basic Parts
    Fave Part Name Type Description Designers Length
    ❤ BBa_K2812004 Basic Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 1458
    ❤ BBa_K2812000 Basic Carbohydrate-binding domain from MpIBP Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 576
    BBa_K2812001 Basic Coding sequence for trunctated Lysostaphin Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 738
    BBa_K2812002 Basic pBAD-ara promoter - Arabinose inducible regulatory promoter Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls 71
    BBa_K2812006 Basic Coding sequence for Pyocin S5 with HlyA and His6-tag Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 2217
    All Parts
    Fave Part Name Type Description Designers Length
    ❤ BBa_K2812005 Composite Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 1496
    BBa_K2812003 Composite Coding sequence for trunctated Lysostaphin regulated by T7-promoter Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls 776
    BBa_K2812007 Composite Coding sequence for Pyosin S5 with HlyA and His6-tag regulated by AraC promoter Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 2288
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