Difference between revisions of "Team:NYMU-Taipei/Parts"

 
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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h1>Parts Overview</h1>
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<p>This year, we constructed plenty of parts that can be categorized into 3 groups:</p>
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<h2>1. The plasmids for FRET Model</h2>
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<p>This group ranges from <a href="http://parts.igem.org/Part:BBa_K2751000">part BBa_K2751000</a> to <a href = "http://parts.igem.org/Part:BBa_K2751007">BBa_K2751007</a>. The parts are the ones that we primarily constructed and utilized in the FRET system. <a href="http://parts.igem.org/Part:BBa_K2751000">BBa_K2751000</a> is a pET32a with its MCS replaced with sequences designed by us. The replacement sequence contains a specifically designed MCS that can be inserted with a FRET protein and a DKK1-binding protein and express the two proteins as a fusion protein under the control of lac operon of pET32a. Hence, <a href="http://parts.igem.org/Part:BBa_K2751003">part BBa_K2751003</a> to <a href="http://parts.igem.org/Part:BBa_K2751007">part BBa_K2751007</a> are all cloned using <a href="http://parts.igem.org/Part:BBa_K2751000">BBa_K2751000.</a></p>
  
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<h2>2. The Submitted Parts</h2>
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<p>Ranging from <a href="http://parts.igem.org/Part:BBa_K2751008">BBa_K2751008</a> to <a href="http://parts.igem.org/Part:BBa_K2751015">BBa_K2751015</a> are the parts that we sent for the medal criteria. Since we have established a system that is suitable for our project but completely different from RFC 10 regulations this year, we can only submit a portion of the parts we have created or there would be too many point mutations to make. While all of them except mEGFP, which is assigned as the improved part, are functional and eligible for silver medal's validated part criteria, <a href="http://parts.igem.org/Part:BBa_K2751013">BBa_K2751013</a> stood out as our favorite composite part and <a href="http://parts.igem.org/Part:BBa_K2751015">BBa_K2751015</a> is our favorite basic part.</p>
  
 
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<h2>3. The plasmids for Cell Model</h2>
 
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<p>The Cell Model has 4 major constructs: from <a href="http://parts.igem.org/Part:BBa_K2751024">BBa_K2751024</a> to <a href="http://parts.igem.org/Part:BBa_K2751027">BBa_K2751027</a>. They are the plasmids that are transformed into HEK293 and DP for fluorescence exhibition.</p>
 
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<h3>Adding parts to the registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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ADD PARTS
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<h3>Inspiration</h3>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h3>What information do I need to start putting my parts on the Registry?</h3>
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<p>The information needed to initially create a part on the Registry is:</p>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h3>Part Table </h3>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM18 NYMU-Taipei</groupparts>
 
<groupparts>iGEM18 NYMU-Taipei</groupparts>
 
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Latest revision as of 02:45, 18 October 2018




Parts Overview

This year, we constructed plenty of parts that can be categorized into 3 groups:

1. The plasmids for FRET Model

This group ranges from part BBa_K2751000 to BBa_K2751007. The parts are the ones that we primarily constructed and utilized in the FRET system. BBa_K2751000 is a pET32a with its MCS replaced with sequences designed by us. The replacement sequence contains a specifically designed MCS that can be inserted with a FRET protein and a DKK1-binding protein and express the two proteins as a fusion protein under the control of lac operon of pET32a. Hence, part BBa_K2751003 to part BBa_K2751007 are all cloned using BBa_K2751000.

2. The Submitted Parts

Ranging from BBa_K2751008 to BBa_K2751015 are the parts that we sent for the medal criteria. Since we have established a system that is suitable for our project but completely different from RFC 10 regulations this year, we can only submit a portion of the parts we have created or there would be too many point mutations to make. While all of them except mEGFP, which is assigned as the improved part, are functional and eligible for silver medal's validated part criteria, BBa_K2751013 stood out as our favorite composite part and BBa_K2751015 is our favorite basic part.

3. The plasmids for Cell Model

The Cell Model has 4 major constructs: from BBa_K2751024 to BBa_K2751027. They are the plasmids that are transformed into HEK293 and DP for fluorescence exhibition.


<groupparts>iGEM18 NYMU-Taipei</groupparts>