Difference between revisions of "Team:TU-Eindhoven/Basic Part"

 
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                <h1 style="text-align: center;">Basic Parts</h1>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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                    <p>The list below shows our Basic Parts. For the medal requirements, we nominated <a href="http://parts.igem.org/Part:BBa_K2812000">BBa_K2812000</a> and <a href="http://parts.igem.org/Part:BBa_K2812004">BBa_K2812004</a> for the silver and gold medal, respectively. As new part for the silver medal, we created the carbohydrate binding domain, <a href="http://parts.igem.org/Part:BBa_K2812000">BBa_K2812000</a>, an adhesin binding strongly to glucose molecules. For the gold medal, TU-Eindhoven 2018 functionally improved on the original truncated lysostaphin <a href="http://parts.igem.org/Part:BBa_K748002">BBa_K748002</a>  created by HIT-Harbin 2012 team in multiple ways. Through codon optimization, expression of the lysostaphin domain has been improved considerably. The other major functional improvement was realized by fusing the truncated lysostaphin to the HlyA secretion domain via a cleavable thrombin linker. In this way, (continuous) secretion via the Type I pathway can be achieved by co-expressing it with the HlyB/D secretion proteins. This avoids the requirement of cell lysis for release of the truncated lysostaphin, improving on the project of iGEM Harbin 2012, as self-destruction of their killer device is no longer necessary. Additionally, this new construct simplifies purification of truncated lysostaphin as running the medium over a nickel affinity column followed by thrombin cleavage results in pure truncated lysostaphin. The part <a href="http://parts.igem.org/Part:BBa_K2812004">BBa_K2812004</a>  has been characterized extensively and the expression, secretion and selective activity of the truncated lysostaphin against S. aureus is demonstrated successfully.</p>
  
  
  
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<h1>Basic Parts</h1>
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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                    <table class="parts-table">
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                      <caption>Basic Parts</caption>
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                      <tr>
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                        <th>Fave Part</th>
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                        <th>Name</th>
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                        <th>Type</th>
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                        <th>Description</th>
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                        <th>Designers</th>
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                        <th>Length</th>
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                        <td><span style="color:red">❤</span></td>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812004">BBa_K2812004</a></td>
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                        <td>Basic</td>
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                        <td>Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA</td>
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                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
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                        <td>1458</td>
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                      </tr>
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                        <td><span style="color:red">❤</span></td>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812000">BBa_K2812000</a></td>
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                        <td>Basic</td>
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                        <td>Carbohydrate-binding domain from MpIBP</td>
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                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
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                        <td>576</td>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812001">BBa_K2812001</a></td>
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                        <td>Basic</td>
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                        <td>Coding sequence for trunctated Lysostaphin</td>
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                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
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                        <td>738</td>
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                      </tr>
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                        <td></td>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812002">BBa_K2812002</a></td>
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                        <td>Basic</td>
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                        <td>pBAD-ara promoter - Arabinose inducible regulatory promoter </td>
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                        <td>Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls</td>
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                        <td>71</td>
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                      </tr>
  
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                        <td></td>
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                        <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2812006">BBa_K2812006</a></td>
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                        <td>Basic</td>
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                        <td>Coding sequence for Pyocin S5 with HlyA and His6-tag </td>
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                        <td>Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls</td>
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                        <td>2217</td>
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<h3>Note</h3>
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
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<h3>Best Basic Part Special Prize</h3>
 
  
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<p> To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
 
  
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
 
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Latest revision as of 02:58, 18 October 2018

Basic Parts

The list below shows our Basic Parts. For the medal requirements, we nominated BBa_K2812000 and BBa_K2812004 for the silver and gold medal, respectively. As new part for the silver medal, we created the carbohydrate binding domain, BBa_K2812000, an adhesin binding strongly to glucose molecules. For the gold medal, TU-Eindhoven 2018 functionally improved on the original truncated lysostaphin BBa_K748002 created by HIT-Harbin 2012 team in multiple ways. Through codon optimization, expression of the lysostaphin domain has been improved considerably. The other major functional improvement was realized by fusing the truncated lysostaphin to the HlyA secretion domain via a cleavable thrombin linker. In this way, (continuous) secretion via the Type I pathway can be achieved by co-expressing it with the HlyB/D secretion proteins. This avoids the requirement of cell lysis for release of the truncated lysostaphin, improving on the project of iGEM Harbin 2012, as self-destruction of their killer device is no longer necessary. Additionally, this new construct simplifies purification of truncated lysostaphin as running the medium over a nickel affinity column followed by thrombin cleavage results in pure truncated lysostaphin. The part BBa_K2812004 has been characterized extensively and the expression, secretion and selective activity of the truncated lysostaphin against S. aureus is demonstrated successfully.

Basic Parts
Fave Part Name Type Description Designers Length
❤ BBa_K2812004 Basic Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 1458
❤ BBa_K2812000 Basic Carbohydrate-binding domain from MpIBP Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 576
BBa_K2812001 Basic Coding sequence for trunctated Lysostaphin Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 738
BBa_K2812002 Basic pBAD-ara promoter - Arabinose inducible regulatory promoter Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls 71
BBa_K2812006 Basic Coding sequence for Pyocin S5 with HlyA and His6-tag Guido Oerlemans, Maxime van den Oetelaar & Mariska Brüls 2217
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