|
|
(14 intermediate revisions by 2 users not shown) |
Line 3: |
Line 3: |
| <div id="page-content"> | | <div id="page-content"> |
| | | |
− | <div class="column full_size judges-will-not-evaluate"> | + | <img src="https://static.igem.org/mediawiki/2018/9/9d/T--NYMU-Taipei--basic-parts-cover.png" class="coverimage"> |
− | <h3>★ ALERT! </h3>
| + | <br> |
− | <p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
| + | <br> |
− | <p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
| + | <br> |
− | </div> | + | |
| | | |
| + | <div class="column"> |
| | | |
− | <div class="clear"></div> | + | <div class="story"> |
| + | <div class="paragraphs"> |
| | | |
| + | <h2>Our Favorite Basic Part: <a href="http://parts.igem.org/Part:BBa_K2751015">BBa_K27510015</a> </h2> |
| + | <h2>Human DKK1 Promoter</h2> |
| | | |
| + | <h3>Description </h3> |
| + | <p>The human DKK1 promoter is the promoter regulating the human DKK1 gene. The promoter is located on human chromosome 10, and will be activated upon DKK1 gene activation. </p> |
| | | |
− | <div class="column full_size"> | + | <h3>Design</h3> |
− | <h1>Basic Parts</h1>
| + | <img src="https://static.igem.org/mediawiki/parts/thumb/7/70/T--NYMU-Taipei--parts-DP1.png/799px-T--NYMU-Taipei--parts-DP1.png" style="width:900px;"> |
− | <p> | + | <img src="https://static.igem.org/mediawiki/parts/3/39/T--NYMU-Taipei--parts-DP2.png" style="width:700px;"> |
− | A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
| + | |
− | </p>
| + | |
| | | |
− | <p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p> | + | <h3>Characterization </h3> |
− | </div>
| + | <p>We used PCR technique to amplify a segment of human chromosome 10 (from 52311944 to 52314294). The estimated length of our DKK1 promoter segment is around 2356bp. </p> |
| | | |
| + | <h3>Functionality Test</h3> |
| + | <p>To test the functionality of the DKK1 promoter, we transfected HEK 293 cells with a plasmid (BBa_K2751024) containing the DKK1 promoter and a mCherry reporter protein. We added various concentrations of testosterone to stimulate the DKK1 promoter. The expression of mCherry was read by IX83 confocal microscope using the same exposure time, and we selected a field full of cells from each condition group to control the number of cells observed. Quantification of cells expressing the mCherry protein was determined via Metamorph multi-wavelength cell scoring. Intensity 10 gray levels above local background were determined as real fluorescence emission.</p> |
| | | |
− | <div class="column full_size"> | + | <style> |
− | <div class="highlight decoration_background">
| + | table, th, td { |
− | <h3>Note</h3>
| + | border: 1px solid black; |
− | <p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
| + | border-collapse: collapse; |
− | </div>
| + | } |
− | </div> | + | </style> |
| | | |
| + | <table style="width:90%"> |
| + | <tr> |
| + | <th>Picture taken under 10x</th> |
| + | <th>Concentration of Testosterone</th> |
| + | <th>Number of cells expressing mCherry protein determined by Metamorph</th> |
| + | </tr> |
| + | <tr> |
| + | <td><img src="https://static.igem.org/mediawiki/parts/1/1a/T--NYMU-Taipei--parts-DP4.png" style="width:400px;"></td> |
| + | <td>0.05ug/mL</td> |
| + | <td>14</td> |
| + | </tr> |
| + | <tr> |
| + | <td><img src="https://static.igem.org/mediawiki/parts/c/c7/T--NYMU-Taipei--parts-DP5.png" style="width:400px;"></td> |
| + | <td>0.5ug/mL</td> |
| + | <td>72</td> |
| + | </tr> |
| + | <tr> |
| + | <td><img src="https://static.igem.org/mediawiki/parts/3/37/T--NYMU-Taipei--parts-DP6.png" style="width:400px;"></td> |
| + | <td>5ug/mL</td> |
| + | <td>77</td> |
| + | </tr> |
| + | </table> |
| + | <p>As the concentration of testosterone rises from 0.05ug/mL to 5ug/mL, the number of cells that express mCherry protein also increases. This shows that testosterone level within a range can stimulate the activation of DKK1 promoter, resulting in increasing expression of mCherry protein. </p> |
| | | |
− | <div class="column full_size"> | + | </div> |
− | <h3>Best Basic Part Special Prize</h3>
| + | |
− | | + | |
− | <p> To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
| + | |
− | | + | |
− | <br><br>
| + | |
− | <b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
| + | |
| </div> | | </div> |
| | | |
| + | <div class="story"> |
| + | <div class="paragraphs"> |
| | | |
− | | + | <h2>List of Basic Parts</h2> |
− | | + | <table style="width:100%"> |
− | | + | <tr> |
− | </html> | + | <th>Part Number</th> |
| + | <th>Description</th> |
| + | <th>Type</th> |
| + | <th>Length(bp)</th> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751015">BBa_K2751015</a></td> |
| + | <td>Human DKK1 promoter</td> |
| + | <td>Coding</td> |
| + | <td>2356</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751008">BBa_K2751008</a></td> |
| + | <td>YPet skeleton</td> |
| + | <td>Reporter</td> |
| + | <td>837</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751011">BBa_K2751011</a></td> |
| + | <td>mEGFP skel</td> |
| + | <td>Reporter</td> |
| + | <td>746</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751000">BBa_K2751000</a></td> |
| + | <td>pET32a-FPFSkel1</td> |
| + | <td>Plasmid</td> |
| + | <td>5413</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751001">BBa_K2751001</a></td> |
| + | <td>pET32a-FPFSkel2</td> |
| + | <td>Plasmid</td> |
| + | <td>5437</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751002">BBa_K2751002</a></td> |
| + | <td>pET32a-FPFSkel1-YPet-Ubc9</td> |
| + | <td>Plasmid</td> |
| + | <td>6592</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751003">BBa_K2751003</a></td> |
| + | <td>pET32a-FPFSkel1 YPet LRP6BP1BP2</td> |
| + | <td>Plasmid</td> |
| + | <td>7816</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751004">BBa_K2751004</a></td> |
| + | <td>pET32a-FPFSkel1 YPet LRP6BP1</td> |
| + | <td>Plasmid</td> |
| + | <td>6904</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751005">BBa_K2751005</a></td> |
| + | <td>pET32a-FPFSkel1 CyPet SUMO1</td> |
| + | <td>Plasmid</td> |
| + | <td>6421</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751006">BBa_K2751006</a></td> |
| + | <td>pET32a-FPFSkel1 CyPet LRP6BP3BP4</td> |
| + | <td>Plasmid</td> |
| + | <td>7821</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751007">BBa_K2751007</a></td> |
| + | <td>pET32a-FPFSkel1 CyPet LRP6BP3</td> |
| + | <td>Plasmid</td> |
| + | <td>6892</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751024">BBa_K2751024</a></td> |
| + | <td>plasmid 1</td> |
| + | <td>Plasmid</td> |
| + | <td>5964</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751025">BBa_K2751025</a></td> |
| + | <td>plasmid 1s</td> |
| + | <td>Plasmid</td> |
| + | <td>6018</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751026">BBa_K2751026</a></td> |
| + | <td>plasmid 2</td> |
| + | <td>Plasmid</td> |
| + | <td>4240</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751027">BBa_K2751027</a></td> |
| + | <td>plasmid 2s</td> |
| + | <td>Plasmid</td> |
| + | <td>4294</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751016">BBa_K2751016</a></td> |
| + | <td>pCMV promoter</td> |
| + | <td>Coding</td> |
| + | <td>290</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751017">BBa_K2751017</a></td> |
| + | <td>LRP6BP1</td> |
| + | <td>Coding</td> |
| + | <td>840</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751018">BBa_K2751018</a></td> |
| + | <td>Ubc9</td> |
| + | <td>Coding</td> |
| + | <td>528</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751019">BBa_K2751019</a></td> |
| + | <td>mEGFP</td> |
| + | <td>Coding</td> |
| + | <td>720</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751020">BBa_K2751020</a></td> |
| + | <td>mEGFP suffix</td> |
| + | <td>Coding</td> |
| + | <td>26</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751021">BBa_K2751021</a></td> |
| + | <td>ALB tag</td> |
| + | <td>Coding</td> |
| + | <td>56</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751022">BBa_K2751022</a></td> |
| + | <td>YPet</td> |
| + | <td>Coding</td> |
| + | <td>777</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2751023">BBa_K2751023</a></td> |
| + | <td>mCherry suffix</td> |
| + | <td>Coding</td> |
| + | <td>23</td> |
| + | </tr> |
| + | </table> |