Difference between revisions of "Team:NAU-CHINA"

 
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 +
{{NAU-CHINA/ResetCSS}}
 
{{NAU-CHINA}}
 
{{NAU-CHINA}}
−
{{NAU-CHINA/header}}
+
{{NAU-CHINA/Hheader}}
−
<html>
+
<html lang="en" xmlns="http://www.w3.org/1999/xhtml">
 
<head>
 
<head>
−
<title>main</title>
+
    <meta charset="utf-8" />
 +
    <title></title>
  
 +
    <style>
 +
      #mw-content-text {
 +
        margin:0 0 0 0;
 +
      }
 +
      body {
 +
        margin: 0;padding: 0;
  
−
<style>
+
        /*overflow-y:hidden;*/
−
html {
+
       
−
  font-family: 'Heveltica','Arial','times new roman',times,sans-serif;
+
        background-color:rgb(14,18,26);
−
  font-size: 16px !important;
+
        /*background-image:url(https://static.igem.org/mediawiki/2018/d/da/T--NAU-CHINA--bgimg.svg);*/
−
}
+
        background-size:100%;
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        background-position:top center;
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        background-repeat:no-repeat;
 +
        /*padding-top:1000px;*/
 +
      }
  
−
h1 {
+
      svg {
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  margin-bottom: 10px !important;
+
            background-color:rgb(14,18,26);
−
}
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            margin-top:-140px;
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      }
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/*
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      ::-webkit-scrollbar {
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        width: 0;
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      }
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*/
  
−
p {
+
      #HomePageBackground {
−
  font-size: 16px;
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        /*background-image: url("https://static.igem.org/mediawiki/2018/9/9c/T--NAU-CHINA--HomePage.svg");
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  text-align: justify;
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        height:5613.43px;
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}
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        background-size: 100%;*/
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        text-align: center;
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        background: rgb(14,18,26);
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        z-index: -99;
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        cursor: default !important;
  
 +
        background-color:rgb(14,18,26);
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        /*background-image:url(https://static.igem.org/mediawiki/2018/d/da/T--NAU-CHINA--bgimg.svg);*/
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        background-size:100%;
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        background-position:top center;
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        background-repeat:no-repeat;
 +
      }
  
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      #CornerIcon {
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        background-color: transparent;
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        position: absolute;
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        width:20vw;
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        top: 2.5vh;
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        left: 2vw;
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        z-index: 1;
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      }
  
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  #Title {
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        font-family:"Michroma","Arial",sans-serif;
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        color:white;
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        font-size:10vw;
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        text-align:center;
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        position:absolute;
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        top:35vh;
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        left:28vw;
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    }
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    #subTitle {
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        font-family:"Lucida Handwriting",sans-serif;
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        color:white;
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        font-size:2vw;
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        text-align:center;
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        position:absolute;
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        top:67vh;
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        left:12.7vw;
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    }
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    #clickDown img {
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        position:absolute;
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        top:75vh;
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        /*left:42vw;*/
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        cursor:pointer;
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        width:15vw;
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    }
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    #clickDown img:hover {
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        top:78vh;
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        transition:.5s;
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    }
 +
    .HomeDiv {
 +
      /*width: 700px;*/
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      position: absolute;
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      color: white;
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    }
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/*
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    .HomeDiv p {
 +
      font-size: 18px !important;
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      text-align: justify;
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      line-height: 1.7em;
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    }
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*/
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    #div1 {top: 84vw;left: 10vw;}
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    #div2 {top: 119.5vw;left: 48.7vw;}
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    #div3 {top: 154.5vw;left: 10vw;}
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    #div4 {top: 189.5vw;left: 63.6vw;}
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    #div5 {top: 227.5vw;left: 10vw;}
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    .HomeStrong {font-size: 30px;color: red;line-height: 1.8em;}
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    .HomeNormal {font-size: 18px;color: red;}
  
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    video {top:150vw;left:50vw;width:30vw;position:absolute;}
  
  
−
.title {
 
−
  text-align:center;
 
  
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#backButton {
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    position: absolute;
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    top: 537vh;
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    left: 92vw;
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    width: 5vw;
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    height: 3vw;
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    border: 3px solid #30343B;
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    border-radius: 8%;
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    background: rgb(14,16,23);
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    line-height: 2.6vw;
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    font-size: 1vw;
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    text-align: center;
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    color: white;
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    font-weight: bold;
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    cursor:pointer;
 
}
 
}
−
 
+
div#backButton:hover {
−
 
+
    border: 2px white solid;
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#main_content {
+
    /*top: 534vh;
−
  margin: 40px 50px 50px 50px;
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    transition: top .5s;*/
 
}
 
}
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      @keyframes animateForward {
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          0%{stroke-dashoffset:900000}
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          100%{stroke-dashoffset:0}
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      }
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      @keyframes animateBackward {
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          0%{stroke-dashoffset:0}
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          100%{stroke-dashoffset:800000}
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      }
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      polyline {
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          animation:animateForward 3600s linear forwards;
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          stroke-dasharray:300 100;
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          /*stroke-opacity:0.5;*/
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      }
  
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      path {
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          animation:animateForward 3600s linear forwards;
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          stroke-dasharray:300 100; 
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      }
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      line {
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          animation:animateForward 3600s linear forwards;
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          stroke-dasharray:300 100;
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          /*stroke-opacity:0.5;*/
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      }
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      circle {
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          fill:red;
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      }
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      #M1 {
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          animation:animateForward 3600s linear forwards;
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          stroke-dasharray:230 100;
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      }     
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      #M2 {
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          animation:animateForward 3600s linear forwards;
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          stroke-dasharray:300 130;
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      }     
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      #M3 {
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          animation:animateForward 3600s linear forwards;
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          stroke-dasharray:300 100;
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      }     
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      #M4 {
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          animation:animateForward 3600s linear forwards;
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          stroke-dasharray:250 180;
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      }     
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      #M5 {
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          animation:animateBackward 3000s linear forwards;
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          stroke-dasharray:270 150;
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      }
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</style>
  
  
−
</style>
 
 
</head>
 
</head>
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<body>
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  <div id="CornerIcon">
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    <a href="https://2018.igem.org/Team:NAU-CHINA">
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      <img src="https://static.igem.org/mediawiki/2018/0/05/T--NAU-CHINA--CornerIcon.png" alt="CornerIcon">
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    </a>
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  </div>
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<div id="clickDown">
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        <img src="https://static.igem.org/mediawiki/2018/d/d2/T--NAU-CHINA--ScrollDown.svg" />
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    </div>
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    <video src="https://static.igem.org/mediawiki/2018/5/59/T--NAU-CHINA--descrition2.mp4" controls='controls'></video>
  
  
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    <!--<img src="https://static.igem.org/mediawiki/2018/5/52/T--NAU-CHINA--HomePageBackground.png" width="100%" alt="HomePage">-->
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 +
    <span id="Title">MOSFET</span>
 +
    <span id="subTitle">Monitoring and Operating System Founded on Engineered T cells</span>
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  <div id="div1" class="HomeDiv">
 +
    <font size="4.5" color="white"><span style="line-height:35px;"> One of the main design goals in engineering is to improve <br/><span class="HomeStrong">SYSTEM STABILITY</span> by reducing noise. <br/>Similarly, in designing synthetically engineered genetic circuits, <br/>we try to reduce the impact of complex intracellular environment on gene circuits, <br/>especially in <span class="HomeStrong">mammalian cells.</span></span></font>
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  </div>
  
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<div id=main_content>
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  <div id="div2" class="HomeDiv">
−
  <h1 class="title"> MOSFET </h1>
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    <font size="4.5" color="white"><span style="line-height:35px;">Therefore, we present our design to solve the problem of <br/><span class="HomeStrong">NOISE</span> in mammalian cells and <span class="HomeStrong">wider applications of synthetic biology </span><br/>on them hoping to make further contribution to <a href="https://2018.igem.org/Team:NAU-CHINA/Human_Practices"><span class="HomeStrong">HUMAN HEALTH</span></a>.</span></font>
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  </div>
  
−
   <h1>Description</h1>
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   <div id="div3" class="HomeDiv">
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  <img src="https://static.igem.org/mediawiki/2018/e/e8/T--NAU-CHINA--loadingicon.jpg" class="img_guide">
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    <font size="4.5" color="white"><span style="line-height:35px;">We, <span class="HomeStrong"> the iGEM team of NAU</span>, <br/>inspired by <a href="https://2018.igem.org/Team:NAU-CHINA/Design"><span class="HomeStrong">MOSFETs</span></a>, simulate the function of a MOSFET <br/>with designed genetic circuits in mammalian cells.</span></font>
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  MOSFET (metal-oxide-semiconductor field-effect transistor) is an essential component in both analog and digital circuits such as analog switches and micro-processors. Inspired by this idea, we built genetic circuit "MOSFETs" in animal T cells which is "Monitoring and Operating System Founded on Engineered T cells". The upstream of this genetic circuit uses synNotch to transduce extracellular signals into cells. The concentration of signals corresponds to different threshold values, and the system can respond accordingly under different concentrations. We achieved some level of logical effects by applying recombinase's reverse mechanism to ensure the uniqueness of downstream output. By using ODE and gillespie algorithms, we conducted validations on mathematical models. Using the concentration of cell surface antigen as gate signal, different recombinase and promoter to adjust threshold value, we conducted experiment validation to measure different promoters and recombinases' response to signals.
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    <font size="4.5" color="white"><span style="line-height:35px;">On top of that, we also carry out simulations <br/>with <a href="https://2018.igem.org/Team:NAU-CHINA/Model"><span class="HomeStrong">MODELS</span></a>of the gene circuits.</span></font>
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    <font size="4.5" color="white"><span style="line-height:35px;">For more iGEMers and teams' communication,<br/> our team develop an <span class="HomeNormal">APP</span> called <a href="https://2018.igem.org/Team:NAU-CHINA/Software"><span class="HomeStrong">iGEMCloud</span></a>.</span></font>
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Latest revision as of 03:57, 18 October 2018

Template:2018_NAU-CHINA

MOSFET Monitoring and Operating System Founded on Engineered T cells
One of the main design goals in engineering is to improve
SYSTEM STABILITY by reducing noise.
Similarly, in designing synthetically engineered genetic circuits,
we try to reduce the impact of complex intracellular environment on gene circuits,
especially in mammalian cells.
Therefore, we present our design to solve the problem of
NOISE in mammalian cells and wider applications of synthetic biology
on them hoping to make further contribution to HUMAN HEALTH.
We, the iGEM team of NAU,
inspired by MOSFETs, simulate the function of a MOSFET
with designed genetic circuits in mammalian cells.
On top of that, we also carry out simulations
with MODELSof the gene circuits.
For more iGEMers and teams' communication,
our team develop an APP called iGEMCloud.
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