Difference between revisions of "Team:CCU Taiwan/Description"

 
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                 <ul>
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                 <ul class="front">
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                     <div id="home_button" style="cursor:pointer;"  onclick="location.href=
 
                     <div id="home_button" style="cursor:pointer;"  onclick="location.href=
 
'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div>
 
'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div>
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                     <li class="title" id="Home"><a>Home</a>
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                         <ul class="sub" id="sub_home">
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                     <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a>
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                             <li class="list" id="home1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Team">Team</a></li>
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                         <ul class="sub" id="sub_home" style="cursor:default;">
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<li class="list" id="home2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions">Attributions</a></li>
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                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a>
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<li class="list" id="home3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Judge">For Judge</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a>
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<div id="home_img">
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a>
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<img src="https://static.igem.org/mediawiki/2018/5/57/T--CCU_Taiwan--home.png"></img>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements"><li class="list" id="home5">Achievements</li></a>
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</div>
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<div id="home_word">
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<p>There's a home for us, always.</p>
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</div>
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                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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                     <li class="title" id="Project"><a href="#">Project</a>
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                     <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a>
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<ul class="sub" id="sub_project">
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<ul class="sub" id="sub_project" style="cursor:default;">
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    <li class="list" id="project1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Description">Description</a></li>
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    <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a>
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<li class="list" id="project2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Design">Design</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Applied_Design"><li class="list" id="project2">Design</li></a>
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<li class="list" id="project3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Results">Results</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a>
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<li class="list" id="project4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements">Achievements</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a>
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<li class="list" id="project5"><a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab ">InterLab</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab"><li class="list" id="project5">InterLab</li></a>
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    <li class="list" id="project6"><a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstration</a></li>
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</ul>
 
</ul>
 
</li>
 
</li>
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                     <li class="title" id="Parts"><a href="#">Parts</a>
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                     <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a>
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                         <ul class="sub" id="sub_parts">
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                         <ul class="sub" id="sub_parts" style="cursor:default;">
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    <li class="list" id="parts1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Parts">Parts</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Parts"><li class="list" id="parts1">Overview</li></a>
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<li class="list" id="parts2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part">Basic Part</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a>
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<li class="list" id="parts3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part">Composite Part</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a>
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<li class="list" id="parts4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Improve">Improved Part</a></li>
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                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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                     <li class="title" id="Modeling"><a href="#">Modeling</a>
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                     <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a>
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                         <ul class="sub" id="sub_modeling">
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                         <ul class="sub" id="sub_modeling" style="cursor:default;">
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                            <li class="list" id="model1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Model">Model</a></li>
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a>
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                            <li class="list" id="model2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Enzyme">Enzyme Activity</a></li>
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding Model</li></a>
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<li class="list" id="model3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Monolignol">Monolignol Binding</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer Model</li></a>
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<li class="list" id="model4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Tools">Tools</a></li>
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                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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                     <li class="title" id="Drylab"><a href="#">Drylab</a>
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                     <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a>
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                         <ul class="sub" id="sub_drylab">
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                         <ul class="sub" id="sub_drylab" style="cursor:default;">
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                            <li class="list" id="drylab1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan">Our Plan</a></li>
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a>
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<li class="list" id="drylab2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering">Engineering</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering"><li class="list" id="drylab2">Production Line</li></a>
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<li class="list" id="drylab3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Safety">Safety</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Safety"><li class="list" id="drylab3">Safety</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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<li class="title" id="Human_Practice"><a href="#">Human Practice</a>
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    <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a>
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                         <ul class="sub" id="sub_human_practice">
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                         <ul class="sub" id="sub_human_practice" style="cursor:default;">
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                             <li class="list" id="human_practice1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices">Human Practice</a></li>
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                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Human Practice</li></a>
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<li class="list" id="human_practice2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engagement">Engagement</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a>
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<li class="list" id="human_practice3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship">Entrepreneurship</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a>
−
<li class="list" id="human_practice4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Business_Model">Business Model</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Integrate"><li class="list" id="human_practice5">Integrated HP</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
−
<li class="title" id="Notebook"><a href="#">Notebook</a>
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    <li class="title" style="cursor:pointer;" id="Notebook"><img class="img_title" src="https://static.igem.org/mediawiki/2018/c/c9/T--CCU_Taiwan--notebook.png"></img><a>Notebook</a>
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                         <ul class="sub" id="sub_notebook">
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                         <ul class="sub" id="sub_notebook" style="cursor:default;">
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                             <li class="list" id="notebook1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li>
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                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook"><li class="list" id="notebook1">Overview</li></a>
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<li class="list" id="notebook2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Applied_Design">Applied Design</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Collaborations"><li class="list" id="notebook2">Collaborations</li></a>
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<li class="list" id="notebook3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments">Experiments</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Protocols"><li class="list" id="notebook3">Protocols</li></a>
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<li class="list" id="notebook4"><a href="https://2018.igem.org/Team:CCU_Taiwan/DATABASE">DATABASE</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments"><li class="list" id="notebook4">Experiments</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Materials"><li class="list" id="notebook5">Materials</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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             </div>
 
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         </nav>
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     </header>
 
     </header>
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</body>
 
  
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<div class="column full_size">
 
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<h1>Description</h1>
 
  
−
<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
+
<div class="backgroundProject">
 +
<div class="indicator">
  
−
</div>
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<div class="pointerProject" id="2"><a href="#ca1">Yeast transformation</a></div>
 +
<div class="pointerProject" id="3"><a href="#ca2">Enzyme production</a></div>
 +
<div class="pointerProject" id="4"><a href="#ca3">Monolignol polymerization</a></div>
 +
<div class="pointerProject" id="5"><a href="#ca4">Human practice</a></div>
  
 +
</div>
 +
<div class="photoDescription"><h1 class="bigtitle">DESCRIPTION<h1></div>
 +
      <div class="content">
 +
            <br><p class="description">&emsp;&emsp;Our product, “LIGGREEN” is a new material that can laminate on paper cups to replace current paper cup laminated with petrochemical materials such as polypropylene and polyethylene. LIGGREEN is a lignin-like polymer. Cups laminated with LIGGREEN is supposed to be waterproof, heat-resistant and eco-friendly.
 +
</p>
 +
      <div class="structuregraph">
 +
        <img id="arrow2" src="https://static.igem.org/mediawiki/2018/a/aa/T--CCU_Taiwan--CCUDescriptionpage4.png" width="100%">
 +
          <div class="transform">
 +
            <p class="first"  id="ca1">Yeast transformation</p>
 +
            <p class="description">&emsp;&emsp;In liginin synthesis, monolignols react with two enzymes, peroxidase and laccase. LIGGREEN, is modelled on <I>Picea abies</I>, which is the most common species used in forestry and possess high content of coniferyl alcohol (G), which is the cheapest monolignol so that it can cost down our product. <br>&emsp;&emsp;We selected peroxidase gene Px16 and Px18 from <I>Picea abies</I>, laccase gene from <I>Pinus taeda</I>.
 +
<I>Pinus taeda</I> and <I>Picea abies</I> are both gymnosperms and have similar ratio of monolignols. Moreover, the reason why we choose laccase from <I>Pinus taed</I>a since it has highest reaction activity at pH 5.0 which is corresponded with peroxidase react with monolignol <I>in vivo</I>. </p>
 +
          </div>
  
 +
          <div class="Enzyme">
 +
            <p class="first" id="ca2">Enzyme production</p>
 +
            <p class="description">&emsp;&emsp;We produce laccase and peroxidase by modified <I>Pichia pastoris (P. pastoris)</I> (strain: SMD1168). There are three main reasons why we select <I>P. pastoris</I> as our host to produce these two enzymes. <br>&emsp;&emsp;First and foremost, laccase and peroxidase are types of glycoprotein that required N-link glycosylation to modify our enzymes, so eukaryotes are more fit than prokaryotes. Second, many researches have applied <I>P. pastoris</I> to secrete plant enzymes, such as HRP. Last but not least, <I>P. pastoris</I> can yield large quantity of protein, it is exactly beneficial for us to produce our product in a large scale. Moreover, we let <I>P. pastoris</I> secret the enzyme by adding a secretion peptide so that we do not need to disrupt the organism in order to decrease the consumables of the following production cost. </p>
 +
          </div>
  
−
<div class="column two_thirds_size">
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          <div class="Polymer">
−
<h3>What should this page contain?</h3>
+
            <p class="first" id="ca3">Monolignol polymerization</p>
−
<ul>
+
            <p class="description">&emsp;&emsp;With the help of laccase, monolignols can form dimers, which have three types of bonds: β-β, β-O-4 and β-5,or oligomers. After that, through reacting with peroxidase, they then start to polymerize, and lignin-like structure would be produced.</p>
−
<li> A clear and concise description of your project.</li>
+
          </div>
−
<li>A detailed explanation of why your team chose to work on this particular project.</li>
+
−
<li>References and sources to document your research.</li>
+
−
<li>Use illustrations and other visual resources to explain your project.</li>
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−
</ul>
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−
</div>
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−
<div class="column third_size" >
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          <div class="HP">
−
<div class="highlight decoration_A_full">
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            <p class="first" id="ca4">Human practice</p>
−
<h3>Inspiration</h3>
+
            <p class="description">&emsp;&emsp;We consulted enterprises and professors about building a business model, including key partners, channels, cost and revenues, etc., to make our product more acceptable for our customers.</p>
−
<p>See how other teams have described and presented their projects: </p>
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Latest revision as of 08:45, 1 December 2018

DESCRIPTION


  Our product, “LIGGREEN” is a new material that can laminate on paper cups to replace current paper cup laminated with petrochemical materials such as polypropylene and polyethylene. LIGGREEN is a lignin-like polymer. Cups laminated with LIGGREEN is supposed to be waterproof, heat-resistant and eco-friendly.

Yeast transformation

  In liginin synthesis, monolignols react with two enzymes, peroxidase and laccase. LIGGREEN, is modelled on Picea abies, which is the most common species used in forestry and possess high content of coniferyl alcohol (G), which is the cheapest monolignol so that it can cost down our product.
  We selected peroxidase gene Px16 and Px18 from Picea abies, laccase gene from Pinus taeda. Pinus taeda and Picea abies are both gymnosperms and have similar ratio of monolignols. Moreover, the reason why we choose laccase from Pinus taeda since it has highest reaction activity at pH 5.0 which is corresponded with peroxidase react with monolignol in vivo.

Enzyme production

  We produce laccase and peroxidase by modified Pichia pastoris (P. pastoris) (strain: SMD1168). There are three main reasons why we select P. pastoris as our host to produce these two enzymes.
  First and foremost, laccase and peroxidase are types of glycoprotein that required N-link glycosylation to modify our enzymes, so eukaryotes are more fit than prokaryotes. Second, many researches have applied P. pastoris to secrete plant enzymes, such as HRP. Last but not least, P. pastoris can yield large quantity of protein, it is exactly beneficial for us to produce our product in a large scale. Moreover, we let P. pastoris secret the enzyme by adding a secretion peptide so that we do not need to disrupt the organism in order to decrease the consumables of the following production cost.

Monolignol polymerization

  With the help of laccase, monolignols can form dimers, which have three types of bonds: β-β, β-O-4 and β-5,or oligomers. After that, through reacting with peroxidase, they then start to polymerize, and lignin-like structure would be produced.

Human practice

  We consulted enterprises and professors about building a business model, including key partners, channels, cost and revenues, etc., to make our product more acceptable for our customers.