Difference between revisions of "Team:CCU Taiwan/Demonstrate"
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<ul class="front"> | <ul class="front"> | ||
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'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div> | 'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div> | ||
| − | <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a | + | <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a> |
<ul class="sub" id="sub_home" style="cursor:default;"> | <ul class="sub" id="sub_home" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements"><li class="list" id="home5">Achievements</li></a> | |
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</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a | + | <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a> |
<ul class="sub" id="sub_project" style="cursor:default;"> | <ul class="sub" id="sub_project" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Applied_Design"><li class="list" id="project2">Design</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab"><li class="list" id="project5">InterLab</li></a> | |
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</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a | + | <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a> |
<ul class="sub" id="sub_parts" style="cursor:default;"> | <ul class="sub" id="sub_parts" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Parts"><li class="list" id="parts1">Overview</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a> | |
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</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a | + | <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a> |
<ul class="sub" id="sub_modeling" style="cursor:default;"> | <ul class="sub" id="sub_modeling" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding Model</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer Model</li></a> | |
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</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a> | + | <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a> |
<ul class="sub" id="sub_drylab" style="cursor:default;"> | <ul class="sub" id="sub_drylab" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering"><li class="list" id="drylab2">Production Line</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Safety"><li class="list" id="drylab3">Safety</li></a> | |
| − | + | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a | + | <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a> |
<ul class="sub" id="sub_human_practice" style="cursor:default;"> | <ul class="sub" id="sub_human_practice" style="cursor:default;"> | ||
| − | <li class="list" id="human_practice1"><a href="https://2018.igem.org/Team:CCU_Taiwan/ | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Human Practice</li></a> |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Integrate"><li class="list" id="human_practice5">Integrated HP</li></a> | |
</ul> | </ul> | ||
</li> | </li> | ||
<li class="title" style="cursor:pointer;" id="Notebook"><img class="img_title" src="https://static.igem.org/mediawiki/2018/c/c9/T--CCU_Taiwan--notebook.png"></img><a>Notebook</a> | <li class="title" style="cursor:pointer;" id="Notebook"><img class="img_title" src="https://static.igem.org/mediawiki/2018/c/c9/T--CCU_Taiwan--notebook.png"></img><a>Notebook</a> | ||
<ul class="sub" id="sub_notebook" style="cursor:default;"> | <ul class="sub" id="sub_notebook" style="cursor:default;"> | ||
| − | <li class="list" id="notebook1"><a href="https://2018.igem.org/Team:CCU_Taiwan/ | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook"><li class="list" id="notebook1">Overview</li></a> |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Collaborations"><li class="list" id="notebook2">Collaborations</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Protocols"><li class="list" id="notebook3">Protocols</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments"><li class="list" id="notebook4">Experiments</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Materials"><li class="list" id="notebook5">Materials</li></a> | |
</ul> | </ul> | ||
</li> | </li> | ||
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</div> | </div> | ||
</nav> | </nav> | ||
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</header> | </header> | ||
| − | <div class=" | + | <div class="indicator"> |
| − | <div class=" | + | |
| + | <div class="pointerProject" id="2"><a href="#ca1">Yeasts & Enzymes</a></div> | ||
| + | <div class="pointerProject" id="3"><a href="#ca2">Properties analysis</a></div> | ||
| + | <div class="pointerProject" id="4"><a href="#ca3">Safety</a></div> | ||
| + | <div class="pointerProject" id="4"><a href="#ca4">Market analysis</a></div> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | <div class="backgroundProject"> | ||
| + | <div class="photoDemonstration"><h1 class="bigtitle">DEMONSTRATION<h1></div> | ||
<div class="content"> | <div class="content"> | ||
| − | + | <p class="first" id="ca1">Yeasts & Enzymes</p> | |
| − | + | <p class="description">  In terms of gene design, our goal is mainly the production of plant enzymes. Therefore, we demonstrated the growth curve of the yeast and ensure the efficiency of the production of enzymes. </p> | |
| − | </p> | + | |
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| − | </p> | + | |
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| − | < | + | <div id="Demonstrate1" class="polaroid"> |
| − | + | <img src="https://static.igem.org/mediawiki/2018/9/92/T--CCU_Taiwan--CCUDemonstrategrowth.jpg" style="width:100%"> | |
| − | + | <div class="container"> | |
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <p>Figure1: Zeocin<sup>TM</sup> selection</p> |
| − | + | </div> | |
| − | + | </div> | |
| + | <br> | ||
| + | <br> | ||
| + | <div id="Demonstrate1-2" class="polaroid"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/6/6b/T--CCU_Taiwan--CCUcurvedata.jpg" style="width:100%"> | ||
| + | <div class="container"> | ||
| + | <p>Figure2: Growth curve data.</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | <div id="Demonstrate1-3" class="polaroid"> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/thumb/1/17/T--CCU_Taiwan--JOEA.PNG/1050px-T--CCU_Taiwan--JOEA.PNG" style="width:100%"> | ||
| + | <div class="container"> | ||
| + | <p>Figure3: Growth curve measurement: Lac1.</p> | ||
| + | </div> | ||
| + | </div> <br> | ||
| + | <br> | ||
| + | <div id="Demonstrate1-4" class="polaroid"> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/thumb/7/7b/T--CCU_Taiwan--JOEC.PNG/1050px-T--CCU_Taiwan--JOEC.PNG" style="width:100%"> | ||
| + | <div class="container"> | ||
| + | <p>Figure4: Growth curve measurement: Px16.</p> | ||
| + | </div> | ||
| + | </div> <br> | ||
| + | <br> | ||
| + | <div id="Demonstrate1-5" class="polaroid"> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/thumb/1/14/T--CCU_Taiwan--JOEB.PNG/1050px-T--CCU_Taiwan--JOEB.PNG" style="width:100%"> | ||
| + | <div class="container"> | ||
| + | <p>Figure5: Growth curve measurement: Px18.</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <br> | ||
| + | <br> | ||
| + | <p class="first" id="ca2">His4 experiment</p> | ||
| + | <p class="description">   In our experiments, we have successfully constructed pGAPZ A containing His4 vectors. As of yet, we have just successfully transformed pGAPZ A_His4_Px18 vector into <I>P. pastoris</I>. In the future, we will do some further researches on this experiment.</p> | ||
| + | |||
| + | <div id="Demonstrate2" class="polaroid"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/6/69/T--CCU_Taiwan--CCUDEMON2.png" style="width:100%"> | ||
| + | <div class="container"> | ||
| + | <p>Figure6: Plasmid digest (EcoRI, Agel) after miniprep</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <br><br> | ||
| + | <div id="Demonstrate3" class="polaroid"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/f/ff/T--CCU_Taiwan--De1.png" style="width:100%"> | ||
| + | <div class="container"> | ||
| + | <p>Figure7: Plasmid digest(SalI) check</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <div id="Demonstrate4" class="polaroid"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/8/80/T--CCU_Taiwan--134.png" style="width:100%"> | ||
| + | <div class="container"> | ||
| + | <p>Figure8: pGAPZ A_His4_Px18 selection with SD plate</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <p class="first" id="ca2">Properties analysis</p> | ||
| + | <p class="description">   For our production methods, we refer to the current material production and get some engineering conditions from the company. Through these engineering conditions, we have designed some modeling and production line. We hope that LIGGREEN will be more in line with business needs. We hope that LIGGREEN will have a place in the market. | ||
| + | <br> | ||
| + |   In terms of engineering properties of LIGGREEN, we also completed two important parts. | ||
</p> | </p> | ||
| − | + | <br> <br> | |
| − | </p> | + | <p class="description"><strong>PART 1.  Modeling</strong></p> |
| − | < | + | <p class="description"><strong>Spontaneous reaction of monolignols & calculation of Flory-Stockmayer Theory of polymerization</strong></p> |
| − | + | ||
| − | < | + | <p class="description">   In our modeling, we constructed a theoretical calculation for our material. Based on published literature, we used Spartan to determine the spontaneous basis for our bond reaction, and then Flory-Stockmayer theory to provide a theoretical estimate of the polymerization. Through the establishment of the model, we can get theoretical data on LIGGREEN. In patent applications and product improvements, we can improve on this estimate through theoretical calculations and material measurements.</p> |
| − | + | <div id="Demonstrate5" class="polaroid"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2018/2/28/T--CCU_Taiwan--CCUmodelbasicpsrt3.png" style="width:120%"> | |
| − | </p> | + | <div class="container"> |
| + | <p>Figure9: Three Bonds based on Spartan Analysis</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <br> <br> | ||
| + | <p class="description"><strong>PART 2.  Structure analysis</strong></p> | ||
| + | <p class="description">   We analyzed the structure of our product. By UV-Visible we proved that the reaction has occurred and the linkage in our product is what we have expected. Also form other analysis, we learned more about the characteristics of our products.</p> | ||
| + | <br> <br> <div id="Demonstrate6" class="polaroid"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/2/29/T--CCU_Taiwan--CCUreaction1.png" style="width:120%"> | ||
| + | <div class="container"> | ||
| + | <p>Figure10: 1st reaction experiment result(coniferyl alcohol react with laccase for 18hr at 25°C) </p> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <div id="Demonstrate7" class="polaroid"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/0/06/T--CCU_Taiwan--CCUreaction2.png" style="width:120%"> | ||
| + | <div class="container"> | ||
| + | <p>Figure11: 2nd reaction experiment result(coniferyl alcohol react with laccase for 18hr at 25°C) </p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <br> <br> <br> | ||
| + | <p class="description"><strong>PART 3.  Production line</strong></p> | ||
| + | <p class="description">   We have designed a production line to produce LIGGREEN. Using existing fermentation tank systems and reaction tank assemblies on the market, we can construct our production line.</p> | ||
| + | <br> <br> | ||
| + | <div id="Demonstrate8" class="polaroid"> | ||
| + | <video id="production" controls> | ||
| + | <source src="https://static.igem.org/mediawiki/2018/8/8e/T--CCU_Taiwan--CCUproduction.mp4" type="video/mp4"> | ||
| + | <source src="https://static.igem.org/mediawiki/2018/c/c4/T--CCU_Taiwan--CCUproduction1221.webm" type="video/webm"> | ||
| + | </video> | ||
| + | <div class="container"> | ||
| + | <p>Figure12: Production line</p> | ||
| + | </div> | ||
| + | </div><br><br> | ||
| + | <p class="first" id="ca3">Safety</a></p><p class="description">  For our production methods, we have designed an effective biological control. In the production line, we have a filter between the fermentation tank and the reaction tank. In addition, our production line will be heat-sterilized after the reaction tank. We have done experiments to prove that our yeast will not cause a biological crisis. | ||
| + | </p><br><i id="like-btn" class="fa fa-thumbs-o-up"><a href="https://2018.igem.org/Team:CCU_Taiwan/Safety">Read more</a></i><br><br> | ||
| + | |||
| + | <br><br><br> | ||
| + | <p class="first" id="ca4">Market analysis</p> <br> | ||
| + | <p class="description">  We have already done a market survey of LIGGREEN, to match the needs of the commodity market. We have built our own business models and various analyses, such as SWOT analysis, STP. Furthermore, we have visited several companies that produce laminated paper cups, to understand their requirements and adjust our product to be suitable for them.(<a href="href="https://2018.igem.org/Team:CCU_Taiwan/Intergrate">Read more in Entrepreniumship</a>)</p> <br> | ||
| + | <br> | ||
| + | <p class="description">  Base on corporate social responsibility, we commit to produce environmental friendly product. Moreover, to bring more welfare to the society, we proposed two policies about food packaging safety and recycling regulations. We consult county councilor to understand the progress of establishing policies and put ours into practice. | ||
| + | (<a href="href="https://2018.igem.org/Team:CCU_Taiwan/Intergrate">Read more in Intergrate HP</a>)</p><br> | ||
| + | |||
| + | <br> | ||
</div> | </div> | ||
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</div> | </div> | ||
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</body> | </body> | ||
| + | |||
| + | <footer class="footer"> | ||
| + | <a href="http://huitong.com.tw"> | ||
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Latest revision as of 08:46, 1 December 2018
DEMONSTRATION
Yeasts & Enzymes
In terms of gene design, our goal is mainly the production of plant enzymes. Therefore, we demonstrated the growth curve of the yeast and ensure the efficiency of the production of enzymes.
Figure1: ZeocinTM selection
Figure2: Growth curve data.
Figure3: Growth curve measurement: Lac1.
Figure4: Growth curve measurement: Px16.
Figure5: Growth curve measurement: Px18.
His4 experiment
In our experiments, we have successfully constructed pGAPZ A containing His4 vectors. As of yet, we have just successfully transformed pGAPZ A_His4_Px18 vector into P. pastoris. In the future, we will do some further researches on this experiment.
Figure6: Plasmid digest (EcoRI, Agel) after miniprep
Figure7: Plasmid digest(SalI) check
Figure8: pGAPZ A_His4_Px18 selection with SD plate
Properties analysis
For our production methods, we refer to the current material production and get some engineering conditions from the company. Through these engineering conditions, we have designed some modeling and production line. We hope that LIGGREEN will be more in line with business needs. We hope that LIGGREEN will have a place in the market.
In terms of engineering properties of LIGGREEN, we also completed two important parts.
PART 1. Modeling
Spontaneous reaction of monolignols & calculation of Flory-Stockmayer Theory of polymerization
In our modeling, we constructed a theoretical calculation for our material. Based on published literature, we used Spartan to determine the spontaneous basis for our bond reaction, and then Flory-Stockmayer theory to provide a theoretical estimate of the polymerization. Through the establishment of the model, we can get theoretical data on LIGGREEN. In patent applications and product improvements, we can improve on this estimate through theoretical calculations and material measurements.
Figure9: Three Bonds based on Spartan Analysis
PART 2. Structure analysis
We analyzed the structure of our product. By UV-Visible we proved that the reaction has occurred and the linkage in our product is what we have expected. Also form other analysis, we learned more about the characteristics of our products.
Figure10: 1st reaction experiment result(coniferyl alcohol react with laccase for 18hr at 25°C)
Figure11: 2nd reaction experiment result(coniferyl alcohol react with laccase for 18hr at 25°C)
PART 3. Production line
We have designed a production line to produce LIGGREEN. Using existing fermentation tank systems and reaction tank assemblies on the market, we can construct our production line.
Figure12: Production line
Safety
For our production methods, we have designed an effective biological control. In the production line, we have a filter between the fermentation tank and the reaction tank. In addition, our production line will be heat-sterilized after the reaction tank. We have done experiments to prove that our yeast will not cause a biological crisis.
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Market analysis
We have already done a market survey of LIGGREEN, to match the needs of the commodity market. We have built our own business models and various analyses, such as SWOT analysis, STP. Furthermore, we have visited several companies that produce laminated paper cups, to understand their requirements and adjust our product to be suitable for them.(Read more in Entrepreniumship)
Base on corporate social responsibility, we commit to produce environmental friendly product. Moreover, to bring more welfare to the society, we proposed two policies about food packaging safety and recycling regulations. We consult county councilor to understand the progress of establishing policies and put ours into practice. (Read more in Intergrate HP)